Abstract
It was shown by us previously that the transcription of the yellow gene can be affected by the promoter of the neighboring gene CG3777, which has a similar expression profile. In the present work, we continued studying the functional interactions between the promoters of the yellow and CG3777 genes in transgenic Drosophila strains. In this work, we used the failure of the yeast activator GAL4 to stimulate transcription from the promoter of the yellow gene for the case when GAL4-binding sites are localized at the 3′-end of the gene. It has been found that, if the 983-bp CG3777 gene promoter is inserted in transgenic strains in the same orientation with the yellow gene promoter, downstream from the sites of the GAL4 activator, the CG3777 promoter provides a strong stimulation of the yellow gene by the GAL4 activator. When the promoters of the yellow and CG3777 genes are inserted in opposite orientations relative to one another, no stimulation of the yellow gene by GAL4 is observed. Additional results obtained in the work demonstrate that the functional interacton between the CG3777 and yellow promoters depends on their mutual orientation and position relative to the GAL4-binding sites.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bulger, M. and Groudine, M., Functional and Mechanistic Diversity of Distal Transcription Enhancers, Cell, 2011, vol. 144, pp. 327–339.
Boutanaev, A.M., Large Clusters of Co-Expressed Genes in the Drosophila Genome, Nature, 2002, vol. 420, no. 6919, pp. 666–669.
Spellman, T. and Rubin, G., Evidence for Large Domains of Similarly Expressed Genes in the Drosophila Genome, J. Biol., 2002, vol. 1, no. 1, pp. 1–5.
Cai, H. and Levine, M., Modulation of Enhancer-Promoter Interactions by Insulators in the Drosophila Embryo, Nature, 1995, vol. 376, no. 6540, pp. 533–536.
Gerasimova, T.I. and Corces, V.G., Boundary and Insulator Elements in Chromosomes, Curr. Opin. Genet. Dev., 1996, vol. 6, no. 2, pp. 185–192.
de Laat, W. and Grosveld, F., Spatial Organization of Gene Expression: The Active Chromatin Hub, Chromosome Res., 2003, vol. 11, no. 5, pp. 447–459.
Pirrotta, V., Vectors for P-Mediated Transformation in Drosophila, Biotechnology, 1988, vol. 10, pp. 437–456.
Geyer, P.K. and Corces, V.G., Separate Regulatory Elements Are Responsible for the Complex Pattern of Tissue-Specific and Developmental Transcription of the yellow Locus in Drosophila melanogaster, Genes Dev., 1987, vol. 1, no. 9, pp. 996–1004.
Lindsley, D.L. and Zimm, G.G., The Genome of Drosophila melanogaster, New York: Academic, 1992.
Karess, R.E. and Rubin, G.M., Analysis of P Transposable Element Functions in Drosophila, Cell, 1984, vol. 38, no. 1, pp. 135–146.
Rubin, G.M. and Spradling, A.C., Genetic Transformation of Drosophila with Transposable Element Vectors, Science, 1982, vol. 218, no. 4570, pp. 348–353.
Spradling, A.C. and Rubin, G.M., Transposition of Cloned P Elements into Drosophila Germ Line Chromosomes, Science, 1982, vol. 218, no. 4570, pp. 341–347.
Pirrotta, V., Steller, H., and Bozzetti, M.P., Multiple Upstream Regulatory Elements Control the Expression of the Drosophila white Gene, EMBO J., 1985, vol. 4, no. 13A, pp. 3501–3508.
Golic, K.G. and Lindquist, S., The FLP Recombinase of Yeast Catalyzes Site-Specific Recombinations in the Drosophila Genome, Cell, 1989, vol. 59, pp. 499–509.
Erokhin, M., Davydova, A., Kyrchanova, O., et al., Insulators Form Gene Loops by Interacting with Promoters in Drosophila, Development, 2011.
Silicheva, M., Drosophila mini-white Model System: New Insights into Positive Position Effects and the Role of Transcriptional Terminators and gypsy Insulator in Transgene Shielding, NAR, 2010.
Pomerantseva, E., Biryukova, I., Silicheva, R., et al., Transposition of Regulatory Elements by P-Element-Mediated Rearrangements in Drosophila melanogaster, Genetics, 2006, vol. 172.
Chopra, V.S., Cande, J., Hong, J.W., and Levine, M., Stalled Hox Promoters as Chromosomal Boundaries, Genes Dev., 2009, vol. 23, no. 13, pp. 1505–1509.
Smith, S.T., Wickramasinghe, P., Olson, A., et al., Genome Wide ChIP-Chip Analyses Reveal Important Roles for CTCF in Drosophila Genome Organization, Dev. Biol., 2009, vol. 328, no. 2, pp. 518–528.
Bartkuhn, M., Straub, T., Herold, M., et al., Active Promoters and Insulators Are Marked by the Centrosomal Protein 190, EMBO J., 2009, vol. 28, no. 7, pp. 877–888.
Jiang, N., Emberly, E., Cuvier, O., and Hart, C.M., Genome-Wide Mapping of Boundary Element-Associated Factor (BEAF) Binding Sites in Drosophila melanogaster Links BEAF to Transcription, Mol. Cell Biol., vol. 29, no. 13, pp. 3556–3568.
Bushey, A.M., Ramos, E., and Corces, V.G., Three Subclasses of a Drosophila Insulator Show Distinct and Cell Type-Specific Genomic Distributions, Genes Dev., 2009, vol. 23, no. 11, pp. 1338–1350.
Nègre, N., Brown, C.D., Shah, P.K., et al., A Comprehensive Map of Insulator Elements for the Drosophila Genome, PLoS Genet., 2010, vol. 6. e1000614.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © D.V. Leman, A.F. Parshikov, P.G. Georgiev, O.G. Maksimenko, 2012, published in Genetika, 2012, Vol. 48, No. 12, pp. 1357–1363.
Rights and permissions
About this article
Cite this article
Leman, D.V., Parshikov, A.F., Georgiev, P.G. et al. Functional interactions between promoters of neighboring yellow and CG3777 genes in Drosophila melanogaster . Russ J Genet 48, 1163–1169 (2012). https://doi.org/10.1134/S1022795412120058
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795412120058