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Russian Journal of Genetics

, Volume 48, Issue 8, pp 823–830 | Cite as

Phenotypic variation of spermatogenesis and a search for associations with genetic polymorphism in 13 inbred mouse strains

  • L. V. Osadchuk
  • A. E. Tupikin
  • I. V. Morozov
  • M. A. Kleshev
  • A. A. Bondar
  • A. V. Osadchuk
Animal Genetics

Abstract

Adult mice of the BALB/cLac, PT, CBA/Lac, DD/He, A/He, SWR, NZB, GR, DBA/2J, CC57Br, C57Bl/6J, A/Sn, and YT inbred strains were tested for the count, motility, and morphology of sperms from the caudal region of the epididymis. The protein-coding regions of the cytochrome P450 aromatase (CYP19a1), estrogen receptor 2 (ESR2), steroidogenic factor 1 (Nr5a1), and sex-determining (Sry) gene were sequenced. A substantial genetic heterogeneity for the genes was observed, as well as a phenotypic variation in spermatogenetic parameters, but the variation was rather discordant. The specifics of the interstrain variation in spermatogenetic parameters indicated that a physiological compensatory mechanism increases certain spermatogenetic parameters when other ones are low to maintain male fertility at a level sufficient for successful reproduction. For instance, a high sperm production compensated for a low sperm motility in DD/He males. In the issue of the protein-coding sequencing of the analyzed genes, 16 various mutations were observed. The decreases in proportion of motile sperms and in their velocity were attributed to mutations (I63T and W133L) of the Sry gene in the DD/He strain.

Keywords

Sperm Motility Sperm Count Motile Sperm Inbred Mouse Strain Caudal Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Jamsai, D. and O’Bryan, M.K., Mouse Models in Male Fertility Research, Asian J. Androl., 2011, vol. 13, pp. 139–151.PubMedCrossRefGoogle Scholar
  2. 2.
    Navarro-Costa, P., Gonçalves, J., and Plancha, C.E., The AZFc Region of the Y Chromosome: At the Crossroads between Genetic Diversity and Male Infertility, Hum. Reprod. Update, 2010, vol. 16, no. 5, pp. 525–542.PubMedCrossRefGoogle Scholar
  3. 3.
    Krausz, C., Chianese, C., Giachini, C., et al., The Y Chromosome-Linked Copy Number Variations and Male Fertility, J. Endocrinol. Invest., 2011, vol. 34, no. 5, pp. 376–382.PubMedGoogle Scholar
  4. 4.
    Carreau, S., Silandre, D., Bourguiba, S., et al., Estrogens and Male Reproduction: A New Concept, Braz. J. Med. Biol. Res., 2007, vol. 40, no. 6, pp. 761–768.PubMedCrossRefGoogle Scholar
  5. 5.
    Akingbemi, B.T., Estrogen Regulation of Testicular Function, Reprod. Biol. Endocrinol., 2005, vol. 3, p. 51, doi:10.1186/1477-7827-3-51.PubMedCrossRefGoogle Scholar
  6. 6.
    Parker, K.L., Rice, D.A., Lala, D.S., et al., Steroidogenic Factor 1: An Essential Mediator of Endocrine Development, Recent Prog. Horm. Res., 2002, vol. 57, pp. 19–36.PubMedCrossRefGoogle Scholar
  7. 7.
    Schimmer, B.P. and White, P.C., Minireview: Steroidogenic Factor 1: Its Roles in Differentiation, Development, and Disease, Mol. Endocrinol., 2010, vol. 24, no. 7, pp. 1322–1337.PubMedCrossRefGoogle Scholar
  8. 8.
    Hewitt, S.C. and Korach, K.S., Oestrogen Receptor Knockout Mice: Roles for Oestrogen Receptors and in Reproductive Tissues, Reproduction, 2003, vol. 125, pp. 143–149.PubMedCrossRefGoogle Scholar
  9. 9.
    Harley, V.R., Clarkson, M.J., and Argentaro, A., The Molecular Action and Regulation of the Testis-Determining Factors, SRY (Sex-Determining Region on the Y Chromosome) and SOX9 [SRY-Related High-Mobility Group (HMG) Box 9], Endocr. Rev., 2003, vol. 24, no. 4, pp. 466–487.PubMedCrossRefGoogle Scholar
  10. 10.
    Kashimada, K. and Koopman, P., Sry: The Master Switch in Mammalian Sex Determination, Development, 2010, vol. 137, pp. 3921–3930.PubMedCrossRefGoogle Scholar
  11. 11.
    Rossi, P., Dolci, S., Albanesi, C., et al., Direct Evidence That the Mouse Sex-Determining Gene Sry Is Expressed in the Somatic Cells of Male Fetal Gonads and in the Germ Cell Line in the Adult Testis, Mol. Reprod. Dev., 1993, vol. 34, no. 4, pp. 369–373.PubMedCrossRefGoogle Scholar
  12. 12.
    O’Bryan, M.K., Takada, S., Kennedy, C.L., et al., Sox8 Is a Critical Regulator of Adult Sertoli Cell Function and Male Fertility, Dev. Biol., 2008, vol. 316, no. 2, pp. 359–370.PubMedCrossRefGoogle Scholar
  13. 13.
    Dubin, R.A. and Ostrer, H., Sry Is a Transcriptional Activator, Mol. Endocrinol., 1994, vol. 8, no. 9, pp. 1182–1192.PubMedCrossRefGoogle Scholar
  14. 14.
    Lazaros, L.A., Xita, N.V., Kaponis, A.I., et al., Estrogen Receptor α and β Polymorphisms Are Associated with Semen Quality, J. Androl., 2010, vol. 31, no. 3, pp. 291–298.PubMedCrossRefGoogle Scholar
  15. 15.
    Gunawan, A., Kaewmala, K., Uddin, M.J., et al., Association Study and Expression Analysis of Porcine ESR1 as a Candidate Gene for Boar Fertility and Sperm Quality, Anim. Reprod. Sci., 2011, vol. 128, nos. 1–4, pp. 11–21.PubMedCrossRefGoogle Scholar
  16. 16.
    Nuti, F. and Krausz, C., Gene Polymorphisms/Mutations Relevant to Abnormal Spermatogenesis, Reprod. Biomed. Online, 2008, vol. 16, no. 4, pp. 504–513.PubMedCrossRefGoogle Scholar
  17. 17.
    Ferlin, A., Raicu, F., Gatta, V., et al., Male Infertility: Role of Genetic Background, Reprod. Biomed. Online, 2007, vol. 14, no. 6, pp. 734–745.PubMedCrossRefGoogle Scholar
  18. 18.
    Schimmer, B.P., Cordova, M., Tsao, J., and Frigeri, C., SF1 Polymorphisms in the Mouse and Steroidogenic Potential, Endocrinol. Res., 2002, vol. 28, no. 4, pp. 519–525.CrossRefGoogle Scholar
  19. 19.
    Schimmer, B.P., Cordova, M., Tsao, J., and Frigeri, C., A Polymorphic Form of Steroidogenic Factor 1 Associated with ACTH Receptor Deficiency in Mouse Adrenal Cell Mutants, Ann. N.Y. Acad. Sci., 2003, vol. 994, pp. 147–153.PubMedCrossRefGoogle Scholar
  20. 20.
    Aitken, R.J., Sperm Function Tests and Fertility, Int. J. Androl., 2006, vol. 29, no. 1, pp. 69–75.PubMedCrossRefGoogle Scholar
  21. 21.
    Daev, E.V. and Dukel’skaya, A.V., The Female Pheromone 2,5-Dimethylpyrazine Induces Sperm Head Abnormalities in Male CBA Mice, Russ. J. Genet., 2003, vol. 39, no. 7, pp. 811–815.CrossRefGoogle Scholar
  22. 22.
    Miller, S.A., Dyckes, D.D., and Polesky, H.F., A Simple Salting out Procedure for Extracting DNA from Human Nucleated Cells, Nucleic Acids Res., 1988, vol. 16, no. 3, p. 1215.PubMedCrossRefGoogle Scholar
  23. 23.
    Jensen, T.K., Andersson, A.M., Jørgensen, N., et al., Body Mass Index in Relation to Semen Quality and Reproductive Hormones among 1.558 Danish Men, Fertil. Steril., 2004, vol. 82, no. 4, pp. 863–870.PubMedCrossRefGoogle Scholar
  24. 24.
    Qin, D.D., Yuan, W., Zhou, W.J., et al., Do Reproductive Hormones Explain the Association between Body Mass Index and Semen Quality? Asian J. Androl., 2007, vol. 9, no. 6, pp. 827–834.PubMedCrossRefGoogle Scholar
  25. 25.
    Kort, H.I., Massey, J.B., Elsner, C.W., et al., Impact of Body Mass Index Values on Sperm Quantity and Quality, J. Androl., 2006, vol. 27, no. 3, pp. 450–452.PubMedCrossRefGoogle Scholar
  26. 26.
    Koloszár, S., Fejes, I., Závaczki, Z., et al., Effect of Body Weight on Sperm Concentration in Normozoospermic Males, Arch. Androl., 2005, vol. 51, no. 4, pp. 299–304.PubMedCrossRefGoogle Scholar
  27. 27.
    Albrecht, K.H. and Eicher, E.M., DNA Sequence Analysis of Sry Alleles (Subgenus Mus) Implicates Misregulation as the Cause of C57BL/6J-Y(POS) Sex Reversal and Defines the Sry Functional Unit, Genetics, 1997, vol. 147, no. 3, pp. 1267–1277.PubMedGoogle Scholar
  28. 28.
    Carlisle, C., Winking, H., Weichenhan, D., and Nagamine, C.M., Absence of Correlation between Sry Polymorphisms and XY Sex Reversal Caused by the M. m. domesticus Y Chromosome, Genomics, 1996, vol. 33, no. 1, pp. 32–45.PubMedCrossRefGoogle Scholar
  29. 29.
    Gronemeyer, H. and Laudet, V., Transcription Factors 3: Nuclear Receptors, Protein Profile, 1995, vol. 2, no. 11, pp. 1173–1308.PubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • L. V. Osadchuk
    • 1
  • A. E. Tupikin
    • 2
  • I. V. Morozov
    • 2
  • M. A. Kleshev
    • 1
  • A. A. Bondar
    • 2
  • A. V. Osadchuk
    • 1
  1. 1.Institute of Cytology and Genetics, Siberian BranchRussian Academy of SciencesNovosibirskRussia
  2. 2.Institute of Chemical Biology and Fundamental Medicine, Siberian BranchRussian Academy of SciencesNovosibirskRussia

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