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Highly efficient transfer and stable expression of two genes upon lentivirus transduction of mesenchymal stem cells from human bone marrow

  • Human Genetics
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Abstract

The efficiency of human bone marrow (BM) mesenchymal stem cell (MSC) transduction with a bicistronic lentivirus vector was estimated, and the stability of transgene expression in genetically modified MSCs was determined. First-passage BM MSCs were capable of efficient transduction with the bicistronic lentivirus vector. The transduction efficiency depended on the multiplicity of infection (MOI), being 64.64 ± 6.5 and 88.6 ± 2.9% at MOI 10 and 20, respectively. The lentivirus transduction efficiency proved independent on the number of passages of a BM MSC culture, and expression of the egfp and dsRed1 transgenes in genetically modified MSCs remained stable for one month of culturing. A comparison showed that the level of egfp and dsRed1 transgene expression was preserved upon hepatogenic differentiation in vitro. The results provide a basis for further development of multigenic modification of human BM MSCs for research and/or therapeutic purposes.

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Authors and Affiliations

  1. Department of Genetics, Belarussian State University, Minsk, 220030, Belarus

    V. V. Grinev & D. V. Posrednik

  2. Belarussian Research and Production Center for Hematology and Transfusiology, Minsk, 220053, Belarus

    I. N. Seviaryn, S. M. Kosmacheva & M. P. Potapnev

Authors
  1. V. V. Grinev
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  2. I. N. Seviaryn
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  3. D. V. Posrednik
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  4. S. M. Kosmacheva
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  5. M. P. Potapnev
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Correspondence to V. V. Grinev.

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Original Russian Text © V.V. Grinev, I.N. Seviaryn, D.V. Posrednik, S.M. Kosmacheva, M.P. Potapnev, 2012, published in Genetika, 2012, Vol. 48, No. 3, pp. 389–400.

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Grinev, V.V., Seviaryn, I.N., Posrednik, D.V. et al. Highly efficient transfer and stable expression of two genes upon lentivirus transduction of mesenchymal stem cells from human bone marrow. Russ J Genet 48, 336–346 (2012). https://doi.org/10.1134/S1022795412030039

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  • DOI: https://doi.org/10.1134/S1022795412030039

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