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Molecular genetic characteristics of the Wx-B1e allele from common wheat and applicability of the DNA markers for its identification

  • Plant Genetics
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Abstract

Molecular genetic characterization of the Wx-B1e allele identified by the authors of the study in the common wheat cultivar Korotyshka was performed. The 804-bp Wx-B1e fragment was cloned and sequenced. Comparison of the sequence obtained with that for the wild-type allele of common wheat (Wx-B1a) demonstrated that Wx-B1e carried the 34-bp insertion, 8-bp deletion, and 23 nucleotide substitutions. BLAST analysis revealed the highest homology with the nucleotide sequences of Wx genes from Triticum spelta and Triticum durum. The amplification variants of four Wx-B1 molecular markers, applied worldwide for testing the collections for different Wx allelic variants, are demonstrated.

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Authors and Affiliations

  1. Center for Molecular Biotechnology, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Moscow, 127550, Russia

    M. G. Divashuk, M. V. Klimushina & G. I. Karlov

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  1. M. G. Divashuk
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  2. M. V. Klimushina
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  3. G. I. Karlov
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Correspondence to M. G. Divashuk.

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Original Russian Text © M.G. Divashuk, M.V. Klimushina, G.I. Karlov, 2011, published in Genetika, 2011, Vol. 47, No.12, pp. 1611–1615.

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Divashuk, M.G., Klimushina, M.V. & Karlov, G.I. Molecular genetic characteristics of the Wx-B1e allele from common wheat and applicability of the DNA markers for its identification. Russ J Genet 47, 1428–1432 (2011). https://doi.org/10.1134/S1022795411120039

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