Abstract
A set of ten microsatellite loci enabling fairly accurate identification of the chum salmon individuals from geographically distant groups was designed at the Laboratory of Genetic Identification, Vavilov Institute of General Genetics, Russian Academy of Sciences. However, identification of the individuals from closely located basins performed using these loci was not sufficiently precise. The present study was focused on the improvement of the resolution of the method through increasing the number microsatellite loci used. In this study, typing of additional microsatellite loci of chum salmon and evaluation of the change of the degree of identification with the increase of the number of microsatellite loci used is described. It was shown that the identification accuracy permanently increased with the increase of the number of microsatellite markers used.
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Original Russian Text © P.K. Afanasiev, L.A. Rubtsova, M.V. Shitova, E.G. Shaykhaev, L.A. Zhivotovsky, 2011, published in Genetika, 2011, Vol. 47, No.11, pp. 1473–1480.
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Afanasiev, P.K., Rubtsova, G.A., Shitova, M.V. et al. Extension of a set of microsatellite markers for more precise identification of chum salmon Oncorhynchus keta walbaum. Russ J Genet 47, 1307–1313 (2011). https://doi.org/10.1134/S1022795411110020
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DOI: https://doi.org/10.1134/S1022795411110020