Skip to main content
SpringerLink
Log in

Analysis of microsatellite variation in the rainbow trout Parasalmo (Oncorhynchus) mykiss from Kamchatka

  • Animal Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Genetic variation of Kamchatka rainbow trout Parasalmo (O.) mykiss was examined using 10 microsatellite DNA loci, and phylogeographic comparison with other representatives of the species across the distribution range was performed. It was demonstrated that Kamchatka populations differed from other geographic groups of rainbow trout in a number of microsatellite loci. These populations also displayed distinct clustering and were characterized by lower genetic diversity. Analysis of a set of 26 different microsatellite loci (personal and literature data) demonstrated that most of the populations within the Kamchatka region were separated from one another, characterized by marked geographic differentiation, and affiliation to certain river basins. In Kamchatka rainbow trout, with high degree of probability, three geographic clusters (northwestern, southwestern, and eastern) were identified. In general, analysis of microsatellite DNA supported the data on low genetic diversity of the Kamchatka group Parasalmo (O.) mykiss, based on the variation estimates for a number of genes of nuclear and mitochondrial DNA, and allozyme loci.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pavlov, D.S., Savvaitova, K.A., Kuzishchin, K.V., et al., Tikhookeanskie blagorodnye lososi i foreli Azii (The Pacific Noble Salmons and Trouts of Asia), Moscow: Nauchnyi Mir, 2001.

    Google Scholar 

  2. Pavlov, D.S., Savvaitova, K.A., and Kuzishchin, K.V., Epigenetic Variations of Life History Strategies in the Red Book Species, Mykiss (Parasalmo mykiss) in Relation to Species Conservation, Dokl. Akad. Nauk, 1999, vol. 367, no. 5, pp. 709–713.

    CAS  Google Scholar 

  3. Zimmerman, K.E., Kuzishchin, K.V., Gruzdeva, M.A., et al., Experimental Determination of the Life History Strategy of the Kamchatka Mykiss Parassalmo mykiss (Walb.)(Salmonidae, Salmoniformes) on the Basis of Analysis of the Sr/Ca Ratio in Otoliths, Dokl. Akad. Nauk, 2003, vol. 389, no. 2, pp. 138–142.

    CAS  Google Scholar 

  4. Osinov, A.G. and Pavlov, S.D., Genetic Similarity between Kamchatka Salmons and American Rainbow Trout, Vopr. Ikhtiol., 1993, vol. 33, no. 5, pp. 626–630.

    Google Scholar 

  5. Pavlov, S.D., Allozyme Variability and Genetic Divergence in Pacific Trout of the Genus Parasalmo from Western Kamchatka, Russ. J. Genet., 2000, vol. 36, no. 9, pp. 1044–1053.

    CAS  Google Scholar 

  6. Pavlov, S.D., Kolesnikov, A.A., Melnikova, M.N., and Ushakova, M.V., Genetic Divergence of Mykiss (Parasalmo (Oncorhynchus) mykiss) from Kamchatka Inferred from Restriction Analysis and Sequencing of mtDNA Cytochrome b Gene, Russ. J. Genet., 2004, vol. 40, no. 12, pp. 1407–1412.

    Article  CAS  Google Scholar 

  7. Melnikova, M.N., Pavlov, S.D., Kolesnikov, A.A., and Petrov, N.B., Screening of Population Genetic SCAR Markers for Mykiss Parasalmo (Oncorhynchus) mykiss from Kamchatka, Russ. J. Genet., 2010, vol. 46, no. 6, pp. 699–704.

    Article  CAS  Google Scholar 

  8. Semenova, A.V., Rubtsova, G.A., Afanas’ev, K.I., and Pavlov, S.D., Analysis of Microsatellite DNA of Rainbow Trout (Parasalmo (Oncorhynchus) mykiss) of Kamchatka: Selection of Loci and Optimization of the Method, Russ. J. Genet., 2010, vol. 46, no. 7, pp. 894–897.

    Article  CAS  Google Scholar 

  9. Pavlov, S.D., Melnikova, M.N., Senchukova, A.L., and Pivovarov, E.A., Development of a New System of Genetic Markers for Kamchatkan Mykiss (Parasalmo (O.) mykiss) as Approach to Study Salmonic Fish Populations, in Molecular Phylogenetics (Proc. 2nd Moscow Int. Conf. (MolPhy-2)), Moscow, 2010, p. 63.

  10. Beachem, T.D., Khai, D.L., and Candy, J.R., Population Structure and Stock Identification of Steelhead Trout (Oncorhynchus mykiss) in British Columbia and the Columbia River Based on Microsatellite Variation, Env. Biol. Fishes., 2004, vol. 69, pp. 95–109.

    Article  Google Scholar 

  11. Lulla, P., Gross, R., and Paaver, T., Genetic Diversity and Differentiation of Imported into Estonia Rainbow Trout (Oncorhynchus mykiss) Strains Based on Microsatellite DNA Variation, J. Agricult. Sci., 2005, vol. 16, pp. 37–42.

    Google Scholar 

  12. Taylor, E., Tamkee, P., Sterling, G., and Hughson, W., Microsatellite DNA Analysis of Rainbow Trout (Oncorhynchus mykiss) from Western Alberta, Canada: Native Status and Evolutionary Distinctiveness of “Athabasca” Rainbow Trout, Conserv. Genet., 2007, vol. 8, pp. 1–15.

    Article  CAS  Google Scholar 

  13. Narum, S.R., Contor, C., Talbot, A., and Powell, M.S., Genetic Divergence of Sympatric Resident and Anadromous Forms of Oncorhynchus mykiss in the Walla Walla River, USA, J. Fish. Biol., 2004, vol. 65, pp. 471–488.

    Article  Google Scholar 

  14. Olsen, J.B., Wuttig, K., Fleming, D., et al., Evidence of Partial Anadromy and Resident-Form Dispersal Bias on a Fine Scale in Populations of Oncorhynchus mykiss, Conserv. Genet., 2006, vol. 7, pp. 613–619.

    Article  Google Scholar 

  15. McCuscer, M., Parkinson, E., and Taylor, E., Mitochondrial DNA Variation in Rainbow Trout (Oncorhynchus mykiss) across Its Native Range: Testing Biogeographical Hypotheses and Their Relevance to Conservation, Mol. Ecol., 2000, vol. 9, pp. 2089–2108.

    Article  Google Scholar 

  16. Williams, R.N., Powell, M.S., Pavlov, S.D., and Proebstel, D.S., Forthcoming: Molecular Genetic Variation among Rainbow Trout Oncorhynchus mykiss Walbaum (Salmonidae, Salmoniformes) from the Kamchatka Peninsula, in Summary of the Eighth Pacific Coast Steelhead Management Meeting, 2002, pp. 27–28.

  17. McPhee, M.V., Utter, F., Stanford, J.A., et al., Population Structure and Partial Anadromy in Oncorhynchus mykiss from Kamchatka: Relevance for Conservation around the Pacific Rim, Ecol. Freshwater Fishes, 2007, vol. 16, no. 4, pp. 539–547.

    Article  Google Scholar 

  18. Afanasiev, K.I., Rubtsova, G.A., Shitova, M.V., et al., Interregional Differentiation of Chum Salmon from Sakhalin and South Kuril Region Inferred from Microsatellite Loci, Russ. J. Genet., 2008, vol. 44, no. 7, pp. 833–840.

    Article  CAS  Google Scholar 

  19. Rubtsova, G.A., Afanasiev, K.I., Malinina, T.V., et al., Differentiation of Chum Salmon Oncorhynchus keta Walbaum Populations as Revealed with Microsatellite and Allozyme Markers: A Comparative Study, Russ. J. Genet., 2008, vol. 44, no. 7, pp. 841–849.

    Article  CAS  Google Scholar 

  20. Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab., 1989.

    Google Scholar 

  21. Olsen, J.B. and Seeb, J.E., Genetic Interpretation of Broad-Scale Microsatellite Polymorphism in Odd-Year Pink Salmon, Trans. Am. Fish. Soc., 1998, vol. 127, pp. 535–550.

    Article  Google Scholar 

  22. Sanchez, J.A., Clabby, C., Ramos, D., et al., Protein and Microsatellite Single Locus Variability in Salmo salar L. (Atlantic Salmon), Heredity, 1996, vol. 77, pp. 423–432.

    Article  PubMed  CAS  Google Scholar 

  23. Olsen, J.B., Wilson, S.L., Kretschemer, E.J., et al., Characterization of 14 Tetranucleotide Microsatellite Loci Derived from Sockeye Salmon, Mol. Ecol., 2000, vol. 9, no. 12, pp. 2185–2187.

    Article  PubMed  CAS  Google Scholar 

  24. Small, M.P., Beachem, T.D., Withler, R.E., and Nelson, R.J., Discriminating Coho Salmon (Oncorhynchus kisutch) Populations within Fraser River, British Columbia, Using Microsatellite DNA Markers, Mol. Ecol., 1998, vol. 7, no. 2, pp. 141–155.

    Article  CAS  Google Scholar 

  25. Smith, C.T., Koop, B.F., and Nelson, R.J., Isolation and Characterization of Coho Salmon (Oncorhynchus kisutch) Microsatellites and Their Use in Other Salmonids, Mol. Ecol., 1998, vol. 7, no. 11, pp. 1614–1616.

    PubMed  CAS  Google Scholar 

  26. Weir, B.S., Genetic Data Analysis, Sunderland: Sinauer, 1993.

    Google Scholar 

  27. Lewis, P.O. and Zaykin, D., Genetic Data Analysis: Computer Program for the Analysis of Allelic Data, 2001, http://lewis.eeb.uconn.edu/lewishome/software.html.

  28. Raymond, M. and Rousset, F., GENEPOP (Version 1.2): Population Genetics Software for Exact Tests and Ecumenicism, J. Hered., 1995, vol. 8, pp. 248–249.

    Google Scholar 

  29. Nei, M., Analysis of Gene Diversity in Subdivided Populations, Proc. Natl. Acad. Sci. USA, 1973, vol. 70, pp. 3321–3323.

    Article  PubMed  CAS  Google Scholar 

  30. Guo, S. and Thompson, E.A., Performing the Exact Test of Hardy-Weinberg Proportion for Multiple Alleles, Biometrics, 1992, vol. 48, pp. 361–372.

    Article  PubMed  CAS  Google Scholar 

  31. Excoffier, L., Laval, G., and Schneider, S., Arlequin ver. 3.0: An Integrated Software Package for Population Genetics Data Analysis, Evol. Bioinform. Online, 2005, vol. 1, pp. 47–50.

    CAS  Google Scholar 

  32. Felsenstein, J., PHYLIP (Phylogeny Inference Package), Version 3.5, 1993, Seattle: Dep. Genet., Univ. Washington.

    Google Scholar 

  33. Page, R.D.M., TREEVIEW: An Application to Display Phylogenetic Trees on Personal Computers, Computer Appl. Biosci., 1996, vol. 12, pp. 357–358.

    CAS  Google Scholar 

  34. Zhivotovsky, L.A., Afanasiev, K.I., Rubtsova, G.I., et al., Development of a DNA Data Base for Solving Reproduction, Identification, and Certification Problems in Pacific Salmon Population Exemplified by Chum Salmon of the Iturup Island, Vopr. Rybolovstva, 2008, no. 1(33), pp. 96–109.

  35. Kuzishchin, K.V., Pavlov, S.D., Gruzdeva, M.A., and Pavlov, D.S., Spawning Population and Reproductive Ecology of the Freshwater Kamchatka Steelhead Parasalmo mykiss in the Basin of the Zhupanova River (East Kamchatka), Vopr. Ikhtiol., 2002, vol. 42, no. 5, pp. 626–638.

    Google Scholar 

  36. Heggenes, J., Beere, M., Tamkee, P., and Taylor, E.B., Genetic Diversity in Steelhead before and after Conservation Hatchery Operation in a Coastal, Boreal River, Trans. Am. Fish. Soc., 2006, vol. 35, pp. 251–267.

    Article  Google Scholar 

  37. Heath, D.D., Pollard, S., and Herbinger, C., Genetic Structure and Relationships among Steelhead Trout (Oncorhynchus mykiss) Populations in British Columbia, Heredity, 2001, vol. 86, pp. 618–627.

    Article  PubMed  CAS  Google Scholar 

  38. Hendry, M.A., Wenburg, J.K., Myers, K.W., and Hendry, A.P., Genetic and Phenotypic Variation through the Migratory Season Provides Evidence for Multiple Populations of Wild Steelhead in the Dean River, British Columbia, Trans. Am. Fish. Soc., 2002, vol. 131, pp. 418–434.

    Article  Google Scholar 

  39. Aguilar, A. and Garza, J.C., A Comparison of Variability and Population Structure for Major Histocompatability Complex and Microsatellite Loci in California Coastal Steelhead (Oncorhynchus mykiss Walbaum), Mol. Ecol., 2006, vol. 15, pp. 923–937.

    Article  PubMed  CAS  Google Scholar 

  40. Ardren, W.R. and Kapuscinski, A.R., Demographic and Genetic Estimates of Effective Population Size (Ne) Reveals Genetic Compensation in Steelhead Trout, Mol. Ecol., 2003, vol. 12, pp. 35–49.

    Article  PubMed  CAS  Google Scholar 

  41. Narum, S.R., Powell, M.S., Evenson, R., et al., Microsatellites Reveal Population Substructure of Klickitat River Native Steelhead and Genetic Divergence from an Introduced Stock, North Am. J. Fish. Management, 2006, vol. 26, pp. 147–155.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ichthyology, Moscow State University, Moscow, 119991, Russia

    S. D. Pavlov & A. V. Semenova

  2. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    G. A. Rubtsova & K. I. Afanasiev

  3. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, 119071, Russia

    A. V. Semenova

Authors
  1. S. D. Pavlov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Semenova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. A. Rubtsova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. I. Afanasiev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. D. Pavlov.

Additional information

Original Russian Text © S.D. Pavlov, A.V. Semenova, G.A. Rubtsova, K.I. Afanasiev, 2011, published in Genetika, 2011, Vol. 47, No.10, pp. 1346–1356.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pavlov, S.D., Semenova, A.V., Rubtsova, G.A. et al. Analysis of microsatellite variation in the rainbow trout Parasalmo (Oncorhynchus) mykiss from Kamchatka. Russ J Genet 47, 1198–1208 (2011). https://doi.org/10.1134/S1022795411100139

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795411100139

Keywords

Search

Navigation