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Structural analysis of genes participating in melibiose fermentation and isocitrate lyase production in Yersinia pestis strains of the main and nonmain subspecies

  • Genetics of Microorganisms
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Abstract

Comparative analysis of nucleotide sequences of genes participating in melibiose fermentation and isocitrate lyase production was conducted in 90 natural Yersinia pestis strains of main and nonmain subspecies. It was ascertained that the lack of the ability to utilize disaccharide melibiose in strains of the main subspecies is caused by integration of the insertion sequence IS285 at 73 bp from the beginning of the structural gene melB that encodes the transport protein galactoside permease. In contrast, strains of nonmain subspecies (caucasica, altaica, hissarica and ulegeica) contain the intact gene melB and are capable of fermenting melibiose. Differences in the manifestation of the other differential trait, production of isocitrate lyase, are connected with the presence in strains of the main species of mutation (insertion of two nucleotides +CC) in the regulatory gene iclR encoding repressor protein of the acetate operon, which is the reason for constitutive synthesis of this enzyme. Strains of nonmain subspecies do not contain mutations in gene iclR, and this correlates in these strains with their capacity for inducible synthesis of isocitrate lyase.

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Authors and Affiliations

  1. Russian Anti-Plague Research Institute “Microbe”, Saratov, 410005, Russia

    G. A. Eroshenko, G. N. Odinokov, L. M. Kookleva, N. Yu. Shavina & V. V. Kutyrev

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  1. G. A. Eroshenko
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  2. G. N. Odinokov
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  3. L. M. Kookleva
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  4. N. Yu. Shavina
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  5. V. V. Kutyrev
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Correspondence to G. A. Eroshenko.

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Original Russian Text © G.A. Eroshenko, G.N. Odinokov, L.M. Kookleva, N.Yu. Shavina, V.V. Kutyrev, 2011, published in Genetika, 2011, Vol. 47, No.10, pp. 1328–1334.

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Eroshenko, G.A., Odinokov, G.N., Kookleva, L.M. et al. Structural analysis of genes participating in melibiose fermentation and isocitrate lyase production in Yersinia pestis strains of the main and nonmain subspecies. Russ J Genet 47, 1181–1187 (2011). https://doi.org/10.1134/S1022795411100073

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  • DOI: https://doi.org/10.1134/S1022795411100073

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