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Molecular genetic analysis of the X-chromosome nuclear envelope attachment region in nurse cells of the malaria mosquitoes Anopheles messeae fall

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Abstract

DNA of the X-chromosomal nuclear envelope attachment region was isolated from malaria mosquito Anopheles messeae Fall. nurse cells by chromosome microdissection. A DNA library of the region was constructed using a plasmid vector. DNA sequencing revealed gene fragments, tandem repeats, and a great variety of transposable elements (TEs). The X-chromosomal nuclear envelope attachment region was concluded to correspond in molecular organization and cytogenetics to diffuse intercalary chromatin.

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References

  1. Steffensen, D.M., Chromosome Architecture and the Interphase Nucleus: Data and Theory on the Mechanism Differentiation and Determination, in Chromosomes Today, Amsterdam: Elsevier, 1977, vol. 6, pp. 247–253.

    Google Scholar 

  2. Stegniy, V.N., Structure Reorganization of Interphase Nuclei in Onto- and Phylogeny of Malaria Mosquito, Dokl. Akad. Nauk SSSR, 1979, vol. 249, no. 5, p. 1231–1234.

    Google Scholar 

  3. Kulichkov, V.A. and Zhimulev, I.F., Analysis of Spatial Organization of Drosophila melanogaster Genome Based on Ectopic Pairing Polytene Chromosomes, Genetika (Moscow), 1976, vol. 12, no. 5, pp. 81–89.

    Google Scholar 

  4. Gaudin, V., Andrey, P., Devinoy, E., et al., Modeling the 3D Functional Architecture of the Nucleus in Animal and Plant Kingdoms, C. R. Biologies, 2009, vol. 332, pp. 937–946.

    Article  PubMed  Google Scholar 

  5. Anan’ina, T.V., Vedernikov, A.E., Vasserlauf, I.E., et al., Visualization of Chromosome Territories in Interphase Nuclei of Ovarian Nurse Cells in Calliphora erythrocephala, Russ. J. Genet., 2005, vol. 41, no. 10, pp. 1106–1112.

    Article  Google Scholar 

  6. Branco, M.R. and Pombo, A., Intermingling of Chromosome Territories in Interphase Suggests Role in Translocations and Transcription-Dependent Associations, PLoS Biol., 2006, vol. 4, pp. 780–788.

    Article  CAS  Google Scholar 

  7. Cremer, T., Cremer, M., Dietzel, S., et al., Chromosome Territories—a Functional Nuclear Land-Scape, Curr. Opin. Cell Biol., 2006, vol. 18, pp. 307–316.

    Article  PubMed  CAS  Google Scholar 

  8. Henikoff, S., Heterochromatin Function in Complex Genomes, Biochim. Biophys. Acta, 2000, vol. 1470, pp. 1–8.

    Google Scholar 

  9. Girod, P.A., Nguyen, D.Q., Calabrese, D., et al., Genome-Wide Prediction of Matrix Attachment Regions That Increase Gene Expression in Mammalian Cells, Nat. Methods, 2007, vol. 4, no. 9, pp. 747–753.

    Article  PubMed  CAS  Google Scholar 

  10. Stegniy, V.N., Arkhitektonika genoma, sistemnye mutatsii i evolyutsiya (Genome Architecture, Systemic Mutations, and Evolution), Novosibirsk: Novosibirsk Gos. Univ., 1993.

    Google Scholar 

  11. Grushko, O.G., Sharakhova, M.V., Shevchenko, A.I., et al., Characterization and Comparative Analysis of DNA from the Pericentric Heterochromatin of Chromosome 2 of Anopheles atroparvus V. Tiel (Culicidae, Diptera), Russ. J. Genet., 2004, vol. 40, no. 10, pp. 1085–1094.

    Article  CAS  Google Scholar 

  12. Grushko, O.G., Sharakhova, M.V., Stegnii, V.N., and Sharakhov, I.V., Molecular Organization of Heterochromatin in Malaria Mosquitoes of the Anopheles maculipennis Subgroup, Gene, 2009, no. 448, pp. 192–197.

  13. Saidzhafarova, A.O., Artemov, G.N., Karamysheva, T.V., et al., Molecular Cytogenetic Analysis of DNA from Pericentric Heterochromatin of Chromosome 2L of Malaria Mosquito of the Anopheles beklemishevi (Culicidae, Diptera), Russ. J. Genet., 2009, vol. 45, no. 1, pp. 49–53.

    Article  CAS  Google Scholar 

  14. Rubtsov, N.B., Alekseenko, A.A., Belyaeva, E.S., et al., Microcloning and Characterization of DNA from Pericentromeric Heterochromatin of Drosophila melanogaster Polytene Chromosomes, Russ. J. Genet., 1999, vol. 35, no. 1, pp. 46–51.

    CAS  Google Scholar 

  15. Kumar, V., Cornel, A.J., and Mukabayire, O., In situ Hybridization to Anopheles Polytene Chromosomes, Molecular Biology of Insect Disease Vectors: A Methods Manual, London: Chapman and Hall, 1997, pp. 337–345.

  16. Stegnii, V.N. and Sharakhova, M.V., Repeated Sequences of Malaria Mosquitoes DNA: Characteristics of High-Repeated DNA Localization of Anopheles messeae Fall., Genetika (Moscow), 1990, vol. 26, no. 7, pp. 775–780.

    Google Scholar 

  17. Stegny, V.N. and Sharakhova, M.V., Systemic Reorganization of the Polytene Chromosomes Architecture at Onto- and Phylogene Malaria Mosquitoes: Structural Peculiarities of Chromosomes Attachment to Nuclear Envelope, Genetika (Moscow), 1991, vol. 27, pp. 574–579.

    Google Scholar 

  18. Shabarina, A.N., Prilepa, E.I., and Glazkov, M.V., Unusual Nucleotide Sequence of a DNA Fragment Isolated from Nuclear Envelopes of Mouse Hepatocytes, Russ. J. Genet., 2006, vol. 42, no. 7, pp. 715–722.

    Article  CAS  Google Scholar 

  19. Sharakhova, M.V., Georgel P., Brusentsova I.V., et al., Genome Mapping and Characterization of the Anopheles gambiae Heterochromatin, BMC Genomics, 2010, no. 11, pp. 459–476.

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Correspondence to G. N. Artemov.

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Original Russian Text © G.N. Artemov, V.N. Stegniy, 2011, published in Genetika, 2011, Vol. 47, No.10, pp. 1307–1314.

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Artemov, G.N., Stegniy, V.N. Molecular genetic analysis of the X-chromosome nuclear envelope attachment region in nurse cells of the malaria mosquitoes Anopheles messeae fall. Russ J Genet 47, 1161–1167 (2011). https://doi.org/10.1134/S1022795411100024

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  • DOI: https://doi.org/10.1134/S1022795411100024

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