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A retroposition assay for the NLR1Cth from midge Chironomus thummi genome in the Chinese hamster ovary cells

  • Molecular Genetics
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Abstract

Non-Long Terminal Repeats (non-LTR or LINE) retrotransposons belong to the class of mobile genetic elements that are transposed into the host genome by reverse transcription of the RNA intermediate. Most of non-LTR retrotransposons contain two open reading frames (ORFs). The ORF1 codes for a gag-like protein, while the ORF2 codes for a reverse transcriptase (RT). We cloned two constructs based on Jockey-like non-LTR retrotransposon from genome Chironomus thummi (NLR1Cth). The retroposition assay performed in Chinese hamster ovary (CHO) cells demonstrated genome integrations of both constructs. The finding that the insect mobile element NLR1Cth is functional in mammalian cells demonstrates that this element possess universal enzymatic machinery allowing for active propagation in the genome of distant taxa. This suggests that the NLR1Cth transposon system may represent a useful tool for genetic analysis and manipulation in vertebrate cells.

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Author information

Authors and Affiliations

  1. Institute of Cytology and Genetics, Novosibirsk, 630090, Russia

    O. Novikova & A. Blinov

  2. Cellular Neurophysiology, Medical School Hannover, Hannover, D-0625, Germany

    E. Papusheva & E. Ponimaskin

  3. Center for Molecular Physiology of the Brain (CMPB), Göttingen, D-37073, Germany

    E. Ponimaskin

Authors
  1. O. Novikova
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  2. E. Papusheva
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  3. E. Ponimaskin
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  4. A. Blinov
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Corresponding author

Correspondence to E. Ponimaskin.

Additional information

Published in Russian in Genetika, 2011, Vol. 47, No. 6, pp. 774–782.

The article is published in the original.

Recipient of a DAAD fellowship.

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Novikova, O., Papusheva, E., Ponimaskin, E. et al. A retroposition assay for the NLR1Cth from midge Chironomus thummi genome in the Chinese hamster ovary cells. Russ J Genet 47, 682–690 (2011). https://doi.org/10.1134/S1022795411060147

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  • DOI: https://doi.org/10.1134/S1022795411060147

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