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Mutational analysis of the ribC gene of Bacillus subtilis

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Abstract

The nucleotide sequence of the ribC gene encoding the synthesis of bifunctional flavokinase/flavine adenine nucleotide (FAD) synthetase in Bacillus subtilis have been determined in a family of riboflavinconstitutive mutants. Two mutations have been found in the proximal region of the gene, which controls the transferase (FAD synthase) activity. Three point mutations and one double mutation have been found (in addition to the two mutations that were detected earlier) in the distal region of the gene, which controls the flavokinase (flavin mononucleotide (FMN) synthase) activity. On the basis of all data known to date, it has been concluded that the identified mutations affect riboflavin and ATP binding sites. No mutations have been found in the PTAN conserved sequence, which forms the magnesium and ATP common binding site and is identical for organisms of all organizational levels, from bacteria too humans.

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Authors and Affiliations

  1. Laboratory of Biophysics, Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russia

    D. V. Karelov, R. A. Kreneva & D. A. Perumov

  2. St. Petersburg State Polytechnical University, St. Petersburg, 195251, Russia

    D. V. Karelov

  3. State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia

    L. Errais Lopes & A. S. Mironov

Authors
  1. D. V. Karelov
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  2. R. A. Kreneva
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  3. L. Errais Lopes
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  4. D. A. Perumov
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  5. A. S. Mironov
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Correspondence to D. V. Karelov.

Additional information

Original Russian Text © D.V. Karelov, R.A. Kreneva, L. Errais Lopes, D.A. Perumov, A.S. Mironov, 2011, published in Genetika, 2011, Vol. 47, No. 6, pp. 856–861.

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Karelov, D.V., Kreneva, R.A., Errais Lopes, L. et al. Mutational analysis of the ribC gene of Bacillus subtilis . Russ J Genet 47, 757–761 (2011). https://doi.org/10.1134/S102279541106010X

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  • DOI: https://doi.org/10.1134/S102279541106010X

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