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Comparative estimation of the EGFP effects on the development of embryos obtained by reciprocal crossing of C57BL/6-Tgn(ACTbEGFP)1Osb/J and C57BL/6 mice

  • Animal Genetics
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Abstract

The effect of enhanced green fluorescent protein (EGFP) on the in vitro viability of early embryos of C57BL/6-Tgn(ACTbEGFP)1Osb/J mice has been studied. The number of viable ova in hemizygous females (−/egfp) has been shown to decrease. Irrespective of the EGFP level, it has no deleterious effect on the early development of embryos obtained by reciprocal crossing of hemizygous (−/egfp) and wild-type (−/−) mice.

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References

  1. Prasher, D.C., Eckenrode, V.K., Ward, W.W., et al., Primary Structure of the Aequorea victoria Green-Fluorescent Protein, Gene, 1992, vol. 111, pp. 229–233.

    Article  PubMed  CAS  Google Scholar 

  2. Chalfie, M., Tu, Y., Euskirchen, G., et al., Green Fluorescent Protein as a Marker for Gene Expression, Science, 1994, vol. 263, pp. 802–805.

    Article  PubMed  CAS  Google Scholar 

  3. Inouye, S. and Tsuji, F., Evidence for Redox Forms of the Aequorea Green Fluorescent Protein, FEBS Lett., 1994, vol. 351, pp. 211–214.

    Article  PubMed  CAS  Google Scholar 

  4. Shimomura, O., Johnson, F., and Saiga, Y., Extraction, Purification and Properties of Aequarin, a Bioluminescent Protein from the Luminous Hydromedusan, Aequarea, J. Cell. Comp. Physiol., 1962, vol. 59, pp. 223–239.

    Article  CAS  Google Scholar 

  5. Johnson, F., Shimomura, O., Saiga, Y., et al., Quantum Efficiency of Cypridina Luminescence, with a Note on That of Aequarea, J. Cell. Comp. Physiol., 1962, vol. 60, pp. 85–104.

    Article  CAS  Google Scholar 

  6. Ikawa, M., Kominami, K., Yoshimura, Y., et al., A Rapid, and Non-Invasive Selection of Transgenic Embryos before Implantation Using Green Fluorescent Protein (GFP), FEBS Lett., 1995, vol. 375, pp. 125–128.

    Article  PubMed  CAS  Google Scholar 

  7. Zernicka-Goetz, M., Pines, J., and McLean Hunter, S., et al., Following Cell Fate in the Living Mouse Embryo, Development, 1997, vol. 124, pp. 1133–1137.

    PubMed  CAS  Google Scholar 

  8. Kato, M., Yamanouchi, K., Ikawa, M., et al., Efficient Selection of Transgenic Mouse Embryos Using EGFP as a Marker Gene, Mol. Reprod. Dev., 1999, vol. 54, pp. 43–48.

    Article  PubMed  CAS  Google Scholar 

  9. Bagis, H. and Keskintepe, L., Application of Green Fluorescent Protein as a Marker for Selection of Transgenic Mouse Embryos before Implantation, Turk. J. Biol., 2001, vol. 25, pp. 123–131.

    CAS  Google Scholar 

  10. Devgan, V., Thomas, M., Ullas, K.S., et al., Embryo Culture-Based Generation of Enhanced Green Fluorescent Protein-Transgenic Mice, Biochem. Biophys. Res. Commun., 2003, vol. 303, pp. 994–1001.

    Article  PubMed  CAS  Google Scholar 

  11. Devgan, V., Rao, M.R.S., and Seshagiri, P.B., Successful Development of Viable Blastocysts from Enhanced Green Fluorescent Protein Transgene-Microinjected Mouse Embryos: Comparison of Culture Media, Mol. Reprod. Dev., 2003, vol. 65, pp. 269–277.

    Article  PubMed  CAS  Google Scholar 

  12. Devgan, V., Rao, M.R.S., and Seshagiri, P.B., Impact of Embryonic Expression of Enhanced Green Fluorescent Protein on Early Mouse Development, Biochem. Biophys. Res. Commun., 2004, vol. 313, pp. 1030–1036.

    Article  PubMed  CAS  Google Scholar 

  13. Sorokin, A.V., Noniashvili, E.M., Sasina, L.K., et al., Green Fluorescent Protein (EGFP) Expression during Early Development of Mouse Embryos, Tsitologiya, 2004, vol. 46, no. 10, pp. 942–943.

    Google Scholar 

  14. Popova, E., Rentzsch, B., Bader, M., and Krivokharchenko, A., Generation and Characterization of a GFP Transgenic Rat Line for Embryological Research, Transgenic Res., 2008, vol. 17, no. 5, pp. 955–963.

    Article  PubMed  CAS  Google Scholar 

  15. Cormack, B.P., Valdivia, R.H., and Falkow, S., FACS-Optimized Mutants of the Green Fluorescent Protein (GFP), Gene, 1996, vol. 173, pp. 33–38.

    Article  PubMed  CAS  Google Scholar 

  16. Okabe, M., Ikawa, M., Kominami, K., et al., ’Green Mice’ as a Source of Ubiquitous Green Cells, FEBS Lett., 1997, vol. 407, pp. 313–319.

    Article  PubMed  CAS  Google Scholar 

  17. Stewart, C.N., Jr., Go with the Glow: Fluorescent Proteins to Light Transgenic Organisms, TRENDS Biotechnol., 2006, vol. 24, no. 4, pp. 155–162.

    Article  PubMed  CAS  Google Scholar 

  18. Liu, H.S., Jan, M.S., and Chou, C.K., et al., Is Green Fluorescent Protein Toxic to the Living Cells?, Biochem. Biophys. Res. Commun., 1999, vol. 260, no. 3, pp. 712–717.

    Article  PubMed  CAS  Google Scholar 

  19. Huang, W.Y., Aramburu, J., Douglas, P.S., and Izumo, S., Transgenic Expression of Green Fluorescence Protein Can Cause Dilated Cardiomyopathy, Nat. Med., 2000, vol. 6, no. 5, pp. 482–483.

    Article  PubMed  CAS  Google Scholar 

  20. Baens, M., Noels, H., Broeckx, V., et al., The Dark Side of EGFP: Defective Polyubiquitination, PLoS ONE, 2006, 1(1): e54.

    Article  PubMed  Google Scholar 

  21. Smirnov, A.A., Shishova, N.V., Sakharova, N.Yu., et al., Localization of Green Fluorescent Protein in Mouse Preimplantation Embryos, Kletochnye tekhnologii v biologii i meditsine (Cell Technologies in Biology and Medicine), 2008, no. 1, pp. 39–44.

  22. Smirnov, A.A. and Vikhlyantseva, E.F., Inheritance of Gene for the Green Fluorescent Protein (GFP) in the C57BL/6-Tg(ACTB-EGFP) Mice Line and Its Expression in Early Embryogenesis, in Fundamental’naya nauka i klinicheskaya meditsina (Fundamental Science and Clinical Medicine), St. Petersburg, 2008, p. 334.

  23. Pratt, H., Experiments with Preimplantation Mouse Embryos, Biologiya razvitiya mlekopitayushchikh: Metody (Developmental Biology of Mammals: Methods), Mank, M., Ed., Moscow: Mir, 1990. pp. 27–64.

    Google Scholar 

  24. Dyban, A.P., Rannee razvitie mlekopitayushchikh (Early Mammalian Development), Leningrad: Nauka, 1988.

    Google Scholar 

  25. Mezhevikina, L.M., Sakharova, N.Yu., and Veprintsev, B.N., Effect of Cryoconservation on Laboratory Early Mouse Embryos, in Problemy sokhraneniya i podderzhanie geneticheskikh kollektsii laboratornykh zhivotnykh (Challenges in Conservation and Maintenance of Genetic Collection of Laboratory Animals), Pushchino, 1991, pp. 102–112.

  26. Zubova, A.N., Bulavina, A.Yu., and Savitskii, A.P., Spectral and Physico-Chemical Characteristics of Green (GFP) and Red (drFR583) Fluorescent Proteins, Usp. Biol. Khim., 2003, vol. 43, pp. 163–224.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    N. Yu. Sakharova & A. A. Smirnov

  2. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    L. M. Mezhevikina & L. A. Fialkovskaya

  3. Pushchino Branch, Moscow State University, Pushchino, Moscow oblast, 142290, Russia

    D. V. Stasenko

Authors
  1. N. Yu. Sakharova
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  2. A. A. Smirnov
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  3. L. M. Mezhevikina
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  4. L. A. Fialkovskaya
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  5. D. V. Stasenko
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Correspondence to N. Yu. Sakharova.

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Original Russian Text © N.Yu. Sakharova, A.A. Smirnov, L.M. Mezhevikina, L.A. Fialkovskaya, D.V. Stasenko, 2011, published in Genetika, 2011, Vol. 47, No. 6, pp. 821–827.

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Sakharova, N.Y., Smirnov, A.A., Mezhevikina, L.M. et al. Comparative estimation of the EGFP effects on the development of embryos obtained by reciprocal crossing of C57BL/6-Tgn(ACTbEGFP)1Osb/J and C57BL/6 mice. Russ J Genet 47, 726–731 (2011). https://doi.org/10.1134/S1022795411050139

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  • DOI: https://doi.org/10.1134/S1022795411050139

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