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Polymorphism of SSR alleles in pear cultivars grown in Belarus

  • Plant Genetics
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Abstract

Using SSR markers designed for Malus × domestica Borkh. genetic polymorphism of 43 pear accessions cultivated in Belarus was examined. A total of 217 alleles were identified with the mean number of 12.8 alleles per marker. The mean PIC value was 0.81; the mean number of informative alleles, 6.49. The heterozygosity level ranged from 0.30 to 0.84. Genetic diversity of SSR alleles in pear and apple genomes was compared. A method of identification of commercial pear cultivars using a set of six SSR markers was suggested.

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References

  1. Genome Mapping and Breeding in Plants: Fruits and Nuts, Berlin: Springer-Verlag, 2007.

  2. Tuz, A.S., On the Origin and Evolution of the Genus Pyrus L., Bot. Zh., 1974 vol. 59, no. 17, pp. 1734–1742.

    Google Scholar 

  3. Bell, R.L, Quamme, H.A., and Layne, R.E.C, Pears, Fruit Breeding vol. 1: Tree and Tropical Fruits, Janick, J. and Moore, J.N.N.Y., Eds., New York, 1996, pp. 441–514.

  4. Likhonos, F.D., Tuz, A.S., and Lobachev, A.Ya., Semechkovye (yablonya, grusha, aiva) (Fruit Crops (Apple, Pear, Quince)) vol. 14 of Kul’turnaya flora SSSR (Cultivated Flora of the Soviet Union), Moscow: Kolos, 1983.

    Google Scholar 

  5. Zielinski, Q.B. and Thompson, M.M., Speciation in Pyrus: Chromosome Number and Meiotic Behavior, Bot. Gaz., 1967 vol. 128, pp. 109–112.

    Article  Google Scholar 

  6. Iketani, H., Manabe, T., Matsuta, N., et al., Incongruence between RFLP’s of Chloroplast DNA and Morphological Classification in East Asian Pear (Pyrus spp.), Genet. Resour. Crop Evol., 1998 vol. 45, pp. 533–539.

    Article  Google Scholar 

  7. Oliveira, C.M., Mota, M., Monte-Corvo, L., et al., Molecular Typing of Pyrus Based on RAPD Markers, Sci. Hort., 1999 vol. 79, pp. 163–174.

    Article  CAS  Google Scholar 

  8. Monte-Corvo, L., Goulao, L., and Oliveira, C., ISSR Analysis of Cultivars of Pear and Suitability of Molecular Markers for Clone Discrimination, J. Am. Soc. Hortic. Sci., 2001 vol. 126, pp. 517–522.

    CAS  Google Scholar 

  9. Monte-Corvo, L., Cabrita, L., Oliveira, C., et al., Assessment of Genetic Relationships among Pyrus Species and Cultivars Using AFLP and RAPD Markers, Genet. Resour. Crop Evol., 2000 vol. 47, pp. 257–265.

    Article  Google Scholar 

  10. Katayama, H. and Uematsu, C., Comparative Analysis of Chloroplast DNA in Pyrus Species: Physical Map and Gene Localization, Theor. Appl. Genet., 2003 vol. 106, pp. 303–310.

    PubMed  CAS  Google Scholar 

  11. Teng, Y., Tanabe, K., Tamura, F., et al., Genetic Relationships of Pyrus Species and Cultivars Native to East Asia Revealed by Randomly Amplified Polymorphic DNA Markers, J. Am. Soc. Hortic. Sci., 2002 vol. 127, pp. 262–270.

    CAS  Google Scholar 

  12. Powell, W., Morgante, M., Andre, C., et al., The Comparison of RFLP, RAPD, AFLP and SSR (Microsatellite) Markers for Germplasm Analysis, Mol. Breed., 1996 vol. 2, pp. 225–238.

    Article  CAS  Google Scholar 

  13. Liebhard, R., Gianfranceschi, L., Koller, B., et al., Development and Characterization of 140 New Microsatellites in Apple (Malus × domestica Borkh.), Mol. Breed., 2002 vol. 10, pp. 217–241.

    Article  CAS  Google Scholar 

  14. Hokanson, S.C. and Lamboy, W.F., Szewc-McFadden, A., et al., Microsatellite (SSR) Variation in a Collection of Malus (Apple) Species and Hybrids, Euphytica, 2001 vol. 118, pp. 281–294.

    Article  CAS  Google Scholar 

  15. Yamamoto, T., Kimura, T., Sawamura, Y., et al., SSRs Isolated from Apple Can Identify Polymorphism and Genetic Diversity in Pear, Theor. Appl. Genet., 2001 vol. 102, pp. 865–870.

    Article  CAS  Google Scholar 

  16. Yamamoto, T., Kimura, T., Soejima, J., et al., Identification of Quince Varieties Using SSR Markers Developed from Pear and Apple, Breed. Sci., 2004 vol. 54, no. 3, pp. 239–244.

    Article  Google Scholar 

  17. Pierantoni, L., Cho, K.H., Shin, I.S., et al., Characterization and Transferability of Apple SSRs to Two European Pear F1 Population, Theor. Appl. Genet., 2004 vol. 109, pp. 1519–1524.

    Article  PubMed  CAS  Google Scholar 

  18. Celton, J.-M., Chagne, D., Tustin, D.S., et al., Update on Comparative Genome Mapping between Malus and Pyrus, BMC Res. Notes, 2009 vol. 2, p. 182.

    Article  PubMed  Google Scholar 

  19. Gasic, K., Han, Y., Kertbundit, S., et al., Characteristics and Transferability of New Apple EST-Derived SSRs to Other Rosaceae Species, Mol. Breed., 2009 vol. 23, pp. 397–411.

    Article  CAS  Google Scholar 

  20. Ghosh, A.K., Lukens, L.N., Hunter, D.M., et al., European and Asian Pear: Simple Sequence Repeat-Polyacrilamide Gel Electrophoresis-Based Analysis of Commercial Important North American Cultivars, Hort. Science, 2006 vol. 41, pp. 304–309.

    CAS  Google Scholar 

  21. Cevik, V. and King, G.J., High-Resolution Genetic Analysis of the Sd-1 Aphid Resistance Locus in Malus spp., Theor. Appl. Genet., 2002 vol. 105, pp. 346–354.

    Article  PubMed  CAS  Google Scholar 

  22. Botstein, D., White, R.L., Skolnik, M., et al., Construction of a Genetic Linkage Map in Man Using Restriction Fragment Length Polymorphisms, Am. J. Hum. Genet., 1980 vol. 32, pp. 314–331.

    PubMed  CAS  Google Scholar 

  23. Nei, M. and Li, W.H., Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases, Proc. Natl. Acad. Sci. USA, 1979 vol. 76, pp. 5269–5273.

    Article  PubMed  CAS  Google Scholar 

  24. Urbanovich, O.Y. and Kazlovskaya, Z.A., Identification of Apple Tree Cultivars Growing in Belarus Using SSR-Markers, Acta Hortic., 2009 vol. 839, pp. 479–486.

    CAS  Google Scholar 

  25. Wüensch, A. and Hormaza, J.I., Characterization of Variability and Genetic Similarity of European Pear Using Microsatellite Loci Developed in Apple, Sci. Hort., 2007 vol. 113, pp. 37–43.

    Article  Google Scholar 

  26. Galli, Z., Halasz, G., Kiss, E., et al., Molecular Identification of Commercial Apple Cultivars with Microsatellite Markers, Hort. Sci., 2005 vol. 40, no. 7, pp. 1974–1977.

    Google Scholar 

  27. Pereira-Lorenzo, S., Ramos-Cabrer, A.M., and Diaz-Hernandez, M.B., Evaluation of Genetic Identity and Variation of Local Apple Cultivars (Malus × domestica Borkh.) from Spain Using Microsatellite Markers, Genet. Resour. Crop Evol., 2007 vol. 54, pp. 405–420.

    Article  CAS  Google Scholar 

  28. This, P., Jung, A., Boccacci, P., et al., Development of a Standard Set of Microsatellite Reference Alleles for Identification of Grape Cultivars, Theor. Appl. Genet., 2004 vol. 109, pp. 1448–1458.

    Article  PubMed  CAS  Google Scholar 

  29. Silfverberg-Dilworth, E., Matasci, C.L., Van de Weg, W.E., et al., Microsatellite Markers Spanning the Apple (Malus × domestica Borkh.) Genome, Tree Genet. Genom, 2006 vol. 2, pp. 202–224.

    Article  Google Scholar 

  30. Oraguzie, N.C., Yamamoto, T., Soejima, J., et al., DNA Fingerprinting of Apple (Malus spp.) Rootstocks Using Simple Sequence Repeats, Plant Breed., 2005 vol. 124, pp. 197–202.

    Article  CAS  Google Scholar 

  31. Richards, C.M., Volk, G.M., Reilley, A.A., et al., Genetic Diversity and Population Structure in Malus sieversii, a Wild Progenitor Species of Domesticated Apple, Tree Genet. Genome, 2009 vol. 6, pp. 339–347.

    Article  Google Scholar 

  32. Patzak, J., Henychova, A., and Paprstein, F., Evaluation of Old and Local Apple (Malus × domestica Borkh.) Varieties from Genetic Resources by Molecular Genetic SSR Analysis, Agriculture, 2009 vol. 55, no. 1, pp. 55–57.

    Google Scholar 

  33. Sivolap, Yu.M., Topchieva, E.A., and Chebotar’, S.V., Identification and Certification of Common Wheat Cultivars Using RAPD and SSRP Data, Russ. J. Genet., 2000 vol. 36, no. 1, pp. 34–40.

    CAS  Google Scholar 

  34. Kozhukhova, N.E. and Sivolap, Yu., Identification and Registration of Maize Genotypes with the Use of Molecular Markers, Russ. J. Genet., 2004 vol. 40, no. 1, pp. 49–55.

    Article  CAS  Google Scholar 

  35. Bocharova, V.R., Kovaleva, I.A., and Mazurenko, L.S., Identification of Genotypes of Grapewine Clones Using Microsatellite Markers, Tsitol. Genet., 2009 vol. 43, no. 6, pp. 8–17.

    PubMed  CAS  Google Scholar 

  36. Dangl, G.S., Yang, J., Golino, D.A., et al., A Practical Method for Almond Cultivar Identification and Parental Analysis Using Simple Sequence Repeat Markers, Euphytica, 2009 vol. 168, pp. 41–48.

    Article  CAS  Google Scholar 

  37. Patocchi, A., Fernandez-Fernandez, F., Evans, K., et al., Development and Test of 21 Multiplex PCRs Composed of SSRs Spanning Most of the Apple Genome, Tree Genet. Genome, 2009 vol. 5, pp. 211–223.

    Article  Google Scholar 

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Authors and Affiliations

  1. Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, 220072, Belarus

    O. Yu. Urbanovich & N. A. Kartel

  2. Institute for Fruit Growing, Samokhvalovitchy, 223013, Minsk region, Belarus

    Z. A. Kazlouvskaya & O. A. Yakimovich

Authors
  1. O. Yu. Urbanovich
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  2. Z. A. Kazlouvskaya
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  3. O. A. Yakimovich
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  4. N. A. Kartel
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Correspondence to O. Yu. Urbanovich.

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Original Russian Text © O.Yu. Urbanovich, Z.A. Kazlouvskaya, O.A. Yakimovich, N.A. Kartel, 2011, published in Genetika, 2011, Vol. 47, No. 3, pp. 349–358.

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Urbanovich, O.Y., Kazlouvskaya, Z.A., Yakimovich, O.A. et al. Polymorphism of SSR alleles in pear cultivars grown in Belarus. Russ J Genet 47, 305–313 (2011). https://doi.org/10.1134/S1022795411030173

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  • DOI: https://doi.org/10.1134/S1022795411030173

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