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Heterologous expression of a synthetic gene encoding a novel hevein-type antimicrobial peptide of Leymus arenarius in Escherichia coli cells

  • Plant Genetics
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Abstract

A novel antifungal peptide, LAMP-1a, was isolated from sand-elymus (Leymus arenarius) seeds. Expression of a synthetic gene encoding this peptide in Escherichia coli cells was obtained. The target peptide was expressed as a fusion with thioredoxin. Identity of the recombinant peptide to native LAMP-1a was confirmed by chromatography, mass spectrometry, and amino acid sequencing. LAMP-1a displayed a high inhibitory activity in respect of a number of phytopathogenic fungi in in vitro assays, which opens up possibilities for the gene encoding it to be used for genetic transformation of plants and for engineering pathogen-resistant crops

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    L. L. Utkina, A. A. Slavokhotova, A. N. Shiyan, T. I. Odintsova & V. A. Pukhal’skiy

  2. National Research Institute for Veterinary Virology and Microbiology, Russian Academy of Agricultural Sciences, Pokrov, Vladimir oblast, 601120, Russia

    E. O. Zhabon

  3. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 177997, Russia

    E. A. Rogozhin, E. V. Grishin & Ts. A. Egorov

Authors
  1. L. L. Utkina
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  2. E. O. Zhabon
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  3. A. A. Slavokhotova
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  4. E. A. Rogozhin
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  5. A. N. Shiyan
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  6. E. V. Grishin
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  7. Ts. A. Egorov
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  8. T. I. Odintsova
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  9. V. A. Pukhal’skiy
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Correspondence to L. L. Utkina.

Additional information

Original Russian Text © L.L. Utkina, E.O. Zhabon, A.A. Slavokhotova, E.A. Rogozhin, A.N. Shiyan, E.V. Grishin, Ts.A. Egorov, T.I. Odintsova, V.A. Pukhal’skiy, 2010, published in Genetika, 2010, Vol. 46, No. 12, pp. 1645–1651.

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Utkina, L.L., Zhabon, E.O., Slavokhotova, A.A. et al. Heterologous expression of a synthetic gene encoding a novel hevein-type antimicrobial peptide of Leymus arenarius in Escherichia coli cells. Russ J Genet 46, 1449–1454 (2010). https://doi.org/10.1134/S1022795410120070

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  • DOI: https://doi.org/10.1134/S1022795410120070

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