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Domain organization of the ORF2 C-terminal region of the German cockroach retroposon R1

  • Molecular Genetics
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Abstract

Using cosmid vector, a gene library of German cockroach Blattella germanica was constructed. From this library, clones containing full-length copies of two subfamilies of R1 retroposons were selected. Retroposons R1 of German cockroach belonging to different subfamilies were shown to be different in domain organization of the ORF2 C-terminal region. For the first time, retroposons transmembrane domains were identified in the sequences of R1. It was demonstrated that two retroposon R1 subfamilies of German cockroach arose as a result of intragenomic divergence rather than via horizontal transfer of alien mobile element into cockroach genome. The differences in domain organization appeared not as a result of saltatory recombination processes, but as a consequence of gradual mutation accumulation, which led to either degeneration, or to domain formation.

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    A. S. Kagramanova, T. V. Kapelinskaya, A. L. Korolev & D. V. Mukha

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  1. A. S. Kagramanova
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  2. T. V. Kapelinskaya
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  3. A. L. Korolev
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  4. D. V. Mukha
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Corresponding author

Correspondence to D. V. Mukha.

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Original Russian Text © A.S. Kagramanova, T.V. Kapelinskaya, A.L. Korolev, D.V. Mukha, 2010, published in Genetika, 2010, Vol. 46, No. 8, pp. 1041–1049.

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Kagramanova, A.S., Kapelinskaya, T.V., Korolev, A.L. et al. Domain organization of the ORF2 C-terminal region of the German cockroach retroposon R1. Russ J Genet 46, 924–931 (2010). https://doi.org/10.1134/S102279541008003X

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  • DOI: https://doi.org/10.1134/S102279541008003X

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