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Localization of denitrification genes in plasmid DNA of bacteria Azospirillum brasilense

  • Genetics of Microorganisms
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Abstract

In an 85-MDa plasmid (p85) of the plant-associated bacteria Azospirillum brasilense Sp245 the genes encoding copper-containing nitrite reductase (nirK); heterodimeric NO-reductase (norCB); NorQ and NorD proteins affecting synthesis and (or) activation of NirK and (or) NO-reductase (norQD); catalytic subunit I of cytochrome c oxidase (ccoN); presumable NO sensor carrying two hemerythrin domains (orf181); and an enzyme, required for synthesis of presumable NO antagonist, homocysteine (metC) were identified. In the same region of p85, orf293 encoding transcriptional regulator of LysR type, orf208 whose protein product carries a formylmethanofuran dehydrogenase subunit E domain, and an orf164 encoding conserved secretory protein with unknown function were also found. Localization of a set of denitrification genes in the plasmid DNA of A. brasilense Sp245 adjacent to IS elements ISAzba1 and ISAzba2 indicates potential mobility of these genes and high probability of their horizontal transfer among populations of rhizospheric bacteria. A region homologous to p85 nirK—orf208-orf181 genes was detected in an 115-MDa plasmid of A. brasilense Sp7 type strain.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganosms, Russian Academy of Sciences, Saratov, 410049, Russia

    L. P. Petrova, O. E. Varshalomidze, A. V. Shelud’ko & E. I. Katsy

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  1. L. P. Petrova
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  2. O. E. Varshalomidze
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  3. A. V. Shelud’ko
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  4. E. I. Katsy
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Correspondence to E. I. Katsy.

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Original Russian Text © L.P. Petrova, O.E. Varshalomidze, A.V. Shelud’ko, E.I. Katsy, 2010, published in Genetika, 2010, Vol. 46, No. 7, pp. 904–910.

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Petrova, L.P., Varshalomidze, O.E., Shelud’ko, A.V. et al. Localization of denitrification genes in plasmid DNA of bacteria Azospirillum brasilense . Russ J Genet 46, 801–807 (2010). https://doi.org/10.1134/S1022795410070045

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  • DOI: https://doi.org/10.1134/S1022795410070045

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