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SNP detection and haplotype analysis in partial sequence of MSTN gene in sheep

  • Animal Genetics
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Abstract

Polymerase chain reaction (PCR) products of the MSTN gene amplified from sixty sheep of nine Chinese indigenous sheep breeds and one imported sheep breed were sequenced to identify the single-nucleotide polymorphisms (SNPs) in a 378-bp fragment including intron 2 and exon 3 of the MSTN gene. A total of fifteen SNPs (A1937C, T1942G, C1956T, A1972C, A1990G, A2008C, A2011G, C2019T, A2025C, A2027C, T2085G, T2173C, C2198T, C2210T and C2213T) were detected among the sixty sequenced individuals and they were all located in intron 2. Twelve haplotypes were identified from these fifteen SNPs, of which haplotype I (CGTCGCGTCCGCTTT) and VIII (ATCAAAACAATTCCC) were the two major and basic ones with frequencies of 12.25% and 77.80%, respectively. Haplotype VIII was distributed in all sheep breeds and all individuals of the meat or meat-wool type sheep breeds were homozygous with respect to this haplotype. This suggests that haplotype VIII might be related to meat production traits in sheep. Haplotype I was only distributed in the fur, lambskin type and fur-meat type sheep breeds. This suggests that haplotype I may have some relationship with fur traits in sheep.

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Authors and Affiliations

  1. Hebei Key Laboratory of Veterinary Preventive Medicine, Department of Animal Science, Hebei Normal University of Science & Technology, Changli, 066600, China

    Y. F. Gong, Z. Z. Liu & X. M. Jin

  2. Department of Animal Science, College of Animal Science and Technology, Hebei Agricultural University, Baoding, 071001, China

    X. L. Li, R. Y. Zhou & L. H. Li

  3. Key Laboratory Animal Genetics and Breeding of the Ministry of Agricultural, State Key Laboratory of AgroBiotechnology, College of Animal Science and Technology, China Agricultural University, Beijing, 100094, China

    Q. Zhang

Authors
  1. Y. F. Gong
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  2. X. L. Li
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  3. Z. Z. Liu
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  4. X. M. Jin
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  5. R. Y. Zhou
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  6. L. H. Li
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  7. Q. Zhang
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Correspondence to X. L. Li.

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Gong, Y.F., Li, X.L., Liu, Z.Z. et al. SNP detection and haplotype analysis in partial sequence of MSTN gene in sheep. Russ J Genet 45, 1454–1457 (2009). https://doi.org/10.1134/S1022795409120084

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  • DOI: https://doi.org/10.1134/S1022795409120084

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