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Multifunctional regulatory mutation in Bacillus subtilis flavinogenesis system

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Abstract

Among Bacillus subtilis riboflavin-resistant mutants we identified one, which differed from other regulatory mutants by overproduction of riboflavin and simultaneous upregulation of the ribC gene encoding flavokinase/FAD-synthase. Genetic and biochemical analysis showed that the ribU1 mutation determines a trans-acting factor that simultaneously regulates activity of riboflavin and truB-ribC-rpsO operons. Regulatory activity of the ribU1 mutation comprises about 10% of Rfn element activity on interaction with flavins. The ribU1 mutation can be presumably ascribed to a gene of the transcriptional regulators family.

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Authors and Affiliations

  1. Department of Molecular and Radiation Biophysics, Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russia

    R. A. Kreneva, D. V. Karelov & D. A. Perumov

  2. Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia

    N. V. Korolkova & A. S. Mironov

Authors
  1. R. A. Kreneva
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  2. D. V. Karelov
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  3. N. V. Korolkova
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  4. A. S. Mironov
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  5. D. A. Perumov
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Corresponding author

Correspondence to A. S. Mironov.

Additional information

Original Russian Text © R.A. Kreneva, D.V. Karelov, N.V. Korolkova, A.S. Mironov, D.A. Perumov, 2009, published in Genetika, 2009, Vol. 45, No. 10, pp. 1420–1424.

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Kreneva, R.A., Karelov, D.V., Korolkova, N.V. et al. Multifunctional regulatory mutation in Bacillus subtilis flavinogenesis system. Russ J Genet 45, 1256–1259 (2009). https://doi.org/10.1134/S1022795409100147

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  • DOI: https://doi.org/10.1134/S1022795409100147

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