Abstract
Plasmid pBS501 was detected in the strain Comamonas sp. BS501. This plasmid specifies high level of induced resistance (5 mM) to cobalt/nickel both in the host strain and in related strains C. testosteroni B-1241 and C. acidovorans B-1251. Hybridization analysis revealed a homology of pBS501 restriction fragments with the only well-characterized operon cnrXYHCBAT that resides in plasmid pMOL28 from Cupriavidus metallidurans CH34. Essential differences in the structural organization of the cobalt/nickel resistance determinant were found between plasmid pBS501 and the cnr operon.
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Nies, D.H., Efflux-Mediated Heavy Metal Resistance in Prokaryotes, FEMS Microbiol. Rev., 2003, vol. 27, no. 3, pp. 314–341.
Saier, M.H.J., Tam, R., Reizer, A., and Reizer, J., Two Novel Families of Bacterial Membrane Proteins Concerned with Nodulation, Cell Division and Transport, Mol. Microbiol., 1994, vol. 11, pp. 841–847.
Mergeay, M., Nies, D., Schlegel, H.G., et al., Alcaligenes eutrophus CH34 Is a Facultative Chemolithotroph with Plasmid- Bound Resistance to Heavy Metals, J. Bacteriol., 1985, vol. 162, pp. 328–334.
Schmidt, T. and Schlegel, H.G., Combined Nickel-Cobalt- Cadmium Resistance Encoded by the ncc Locus of Alcaligenes xylosoxidans 31A, J. Bacteriol., 1994, vol. 176, pp. 7045–7054.
Stoppel, R.-D. and Schlegel, H.G., Nickel-Resistant Bacteria from Antropogenically Nickel-Polluted and Naturally Nickel-Percolated Ecosystems, Appl. Environ. Microbiol., 1995, vol. 61, no. 6, pp. 2276–2285.
Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab., 1989.
Sambrook, J. and Russel, D.W., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab., 2001, vol. 1-3.
Lane, J., 16S/23S rRNA Sequencing, Nucleic Acid Techniques in Bacterial Systematics, Stackebrandt, E. and Goodfellow, M., Eds., Chichester: Wiley, 1991, pp. 115–175.
Werle, E., Schneider, C., Renner, M., et al., Convenient Single-Step, One Tube Purification of PCR Products for Direct Sequencing, Nucleic Acids Res., 1994, vol. 22, no. 20, pp. 4354–4355.
Van de Peer, Y., Broeck, I., de Rijk, P., and de Wachter, R., Database on the Structure of Small Ribosomal Subunit RNA, Nucleic Acids Res., 1994, vol. 22, no. 17, pp. 3488–3494.
Siunova, T.V., Kochetkov, V.V., Validov, Sh. Z., et al., The Production of Phenazine Antibiotics by the Pseudomonas aureofaciens Strain with Plasmid-Controlled Resistance to Cobalt and Nickel, Mikrobiologiya, 2002, vol. 71, no. 6, pp. 778–785.
Siunova, T.V., Anokhina, T.O., Mashukova, A.V., et al., Rhizosphere Strain of Pseudomonas chlororaphis Capable of Degrading Naphthalene in the Presence of Cobalt/Nickel, Mikrobiologiya, 2007, vol. 76, no. 1, pp. 1–7.
Mergeay, M., Monchy, S., Vallaeys, T., et al., Ralstonia metallidurans, a Bacterium Specifically Adapted to Toxic Metals: Towards a Catalogue of Metal- Responsive Genes, FEMS Microbiol. Rev., 2003, vol. 27, no. 3, pp. 385–411.
Wu, J., Jiang, C., Wang, B., et al., Novel Partial Reductive Pathway for 4-Chloronitrobenzene and Nitrobenzene Degradation in Comamonas sp. Strain CNB-1, Appl. Environ. Microbiol., 2006, vol. 72, no. 3, pp. 1759–1765.
Tralau, T., Cook, A.M., and Ruff, J., Map of the IncP1β Plasmid pTSA Encoding the Widespread Genes (tsa) for p-Toluenesulfonate Degradation in Comamonas testosterone T-2, Appl. Environ. Microbiol., 2001, vol. 67, pp. 1508–1516.
Siddiqui, R.A. and Benthin, K., Cloning of pMOL28-Encoded Nickel Resistance Genes End Expression of the Genes in Alcaligenes eutrophus and Pseudomonas sp., J. Bacteriol., 1989, vol. 171, pp. 5071–5078.
Munkelt, D., Grass, G., and Nies, D.H., The Chromosomally Encoded Cation Diffusion Facilitator Proteins DmeF and FieF from Wautersia metallidurans CH34 Are Transporters of Broad Metal Specificity, J. Bacteriol., 2004, vol. 186, no. 23, pp. 8036–8043.
Siddiqui, R.A., Schlegel, H.G., and Meyer, M., Inducible and Constitutive Expression of pMOL28-Encoded Nickel Resistance in Alcaligenes eutrophus N9A, J. Bacteriol., 1988, vol. 170, no. 9, pp. 4188–4193.
Nies, A., Nies, D.H., and Silver, S., Cloning and Expression of Plasmid Genes Encoding Resistance to Chromate and Cobalt in Alcaligenes eutrophus, J. Bacteriol., 1989, vol. 171, no. 9, pp. 5065–5070.
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Original Russian Text © T.V. Siunova, A.V. Siunov, V.V. Kochetkov, A.M. Boronin, 2009, published in Genetika, 2009, Vol. 45, No. 3, pp. 336–341.
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Siunova, T.V., Siunov, A.V., Kochetkov, V.V. et al. The cnr-like operon in strain Comamonas sp. encoding resistance to cobalt and nickel. Russ J Genet 45, 292–297 (2009). https://doi.org/10.1134/S1022795409030053
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DOI: https://doi.org/10.1134/S1022795409030053