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Clonal and population RAPD variation of cercariae obtained from Bucephalus polymorphus sporocysts (Trematoda: Bucephalidae)

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Abstract

Three arbitrary primers produced 114 RAPD markers for 37 cercariae from nine Bucephalus polymorphus sporocysts obtained from three Dreissena polymorpha mollusks, which were collected in two water reservoirs of the Volga basin. Analysis of the RAPD patterns established a unique genotype for each cercaria. The topology of an UPGMA dendrogram did not reliably differentiate the cercaria according to the corresponding sporocysts. However, three groups of genotypes were isolated and corresponded to the host mollusks, indicating that each cercaria clone had a different genotype set. A within-sporocyst variation made the greatest contribution (53.0%) to the total RAPD diversity, while the contributions of within-host and between-host variations to the total diversity were equal (23.5%). Cercariae isolated from two mollusks of the Rybinskoe Water Reservoir were more similar to each other than to cercariae from the geographically distant Gor’kovskoe Water Reservoir. Possible causes and distribution specifics of the observed genetic diversity of B. polymorphus are discussed.

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia

    A. V. Korsunenko & S. K. Semyenova

  2. Papanin Institute of Biology of Inland Water Bodies, Russian Academy of Sciences, Borok, Yaroslavl Oblast, 152742, Russia

    A. V. Tyutin

Authors
  1. A. V. Korsunenko
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  2. A. V. Tyutin
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  3. S. K. Semyenova
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Correspondence to A. V. Korsunenko.

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Original Russian Text © A.V. Korsunenko, A.V. Tyutin, S.K. Semyenova, 2009, published in Genetika, 2009, Vol. 45, No. 1, pp. 73–80.

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Korsunenko, A.V., Tyutin, A.V. & Semyenova, S.K. Clonal and population RAPD variation of cercariae obtained from Bucephalus polymorphus sporocysts (Trematoda: Bucephalidae). Russ J Genet 45, 63–69 (2009). https://doi.org/10.1134/S1022795409010098

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