Abstract
The effect of antisense oligonucleotides specific to mRNA of the proapoptotic gene harakiri (Hrk) on the development of mouse SAMP1 (senescence-accelerated mouse prone) and (C57BL/6J × DBA/2J)F1 preimplantation embryos cultured in vitro was investigated. The SAMP1 mice are characterized by genetically determined decrease of fertility along with the highly frequent disturbance of embryonic development. Reproduction indices of the (C57BL/6J × DBA/2J) hybrids lie within the normal range. Because of this, preimplantation abnormalities in this line were induced by the action of proapoptotic agent bleomycine. It was demonstrated that antisense inhibition of the Hrk expression had no effect on the frequency of genetically determined abnormalities of early embryonic development in SAMP1 mice. In case of induced abnormalities, addition of oligonucleotides specific to mRNA of proapoptotic Hrk gene influenced the number of abnormalities, and at the same time, improved the quality of survived embryos via increasing the blastocyst hatching.
References
Schultz, R.M., Davis, W., Stein, P., and Svoboda, P., Reprogramming of Gene Expression during Preimplantation Development, J. Exptl. Zool., 1999, vol. 285, pp. 276–282.
O’Neill, C., Role of Autocrine Mediators in the Regulation of Embryo Viability: Lessons from Animal Models, J. Assist. Reprod. Genet., 1998, vol. 15, no. 8, pp. 460–465.
Agarwal, A., Gupta, S., and Sharma, R., Oxidative Stress and Its Implications in Female Infertility—a Clinician’s Perspective, Reprod. Biomed. Online, 2005, vol. 11, no. 5, pp. 641–650.
Exley, G.E., Tang, C., McElhinny, A.S., and Warner, C.M., Expression of Caspase and Bcl-2 Apoptotic Family Members in Mouse Preimplantation Embryo, Biol. Reprod., 1999, vol. 61, pp. 231–239.
Van Blerkom, J., Davis, P., and Alexander, S., A Microscopic and Biochemical Study of Fragmentation Phenotypes in Stage-Appropriate Human Embryos, Hum. Reprod., 2001, vol. 16, no. 4, pp. 719–729.
Jurisicova, A., Antenos, M., Varmuza, S., et al., Expression of Apoptosis-Related Genes during Human Preimplantation Embryo Development: Potential Roles for the Harakiri Gene Product and Caspase-3 in Blastomere Fragmentation, Mol. Hum. Reprod, 2003, vol. 9, no. 3, pp. 133–141.
Jurisicova, A., Latham, K.E., Casper, R.F., and Varmuza, S.L., Expression and Regulation of Genes Associated with Cell Death during Murine Preimplantation Embryo Development, Mol. Reprod. Dev., 1998, vol. 51, pp. 243–253.
Inohara, N., Ding, L., Chen, S., and Nuenz, G., Harakiri, a Novel Regulator of Cell Death, Encodes a Protein That Activates Apoptosis and Interects Selectively with Survival-Promoting Proteins Bcl-2 and Bcl-XL, EMBO J., 1997, vol. 16, no. 7, pp. 1686–1694.
Stein, C.A. and Cohen, J.S., Oligodeoxynucleotides as Inhibitors of Gene Expression: A Review, Cancer Res., 1988, vol. 48, no. 10, pp. 2659–2668.
Mullen, P. and McPhillips, F., MacLeod K. et al. Antisense Oligonucleotide Targeting of Raf-1: Importance of Raf-1 mRNA Expression Levels and Raf-1-Dependent Signaling in Determining Growth Response in Ovarian Cancer, Clin. Cancer Res., 2004, vol. 10, pp. 2100–2108.
Phillips, M.I. and Kimura, B., Antisense Therapeutics for Hypertension: Targeting the Renin-Angiotensin System, Methods Mol. Med., 2005, vol. 106, pp. 51–68.
Ball, H.A., Van Scott, M.R., and Robinson, C.B., Sense and Antisense: Therapeutic Potential of Oligonucleotides and Interference RNA in Asthma and Allergic Disorders, Clin. Rev. Allergy Immunol., 2004, vol. 27, no. 3, pp. 207–217.
Geller, B.L., Antibacterial Antisense, Curr. Opin. Mol. Ther., 2005, vol. 7, no. 2, pp. 109–113.
Wingard, L.B., Brody, T.M., Larner, J., and Schwartz, A., Human Pharmacology: Molecular-to-Clinic, Saint Louis: Mosby-Year Book, 1991.
Quinn, P., Barros, C., and Whittingam, D.G., Preservation of Hamster Oocytes to Assay the Fertilization Capacity for Human Spermatozoa, J. Reprod. Fertil., 1982, vol. 66, no. 1, pp. 161–168.
Miyamoto, H., Manabe, N., Mitani, Y., et al., Female Reproductive Properties and Prenatal Development of a Senescence-Accelerated Mouse Strain, J. Exptl. Zool., 1995, vol. 272, no. 2, pp. 116–122.
Ribas, R.C., Taylor, J.E., McCorquodale, C., et al., Effect of Zona Pellucida Removal on DNA Methylation in Early Mouse Embryos, Biol. Reprod., 2006, vol. 74, no. 2, pp. 307–313.
Zander, D.L., Thompson, J.C., and Lane, M., Disturbances in Mouse Embryo Development and Viability Caused by Ammonium Are More Severe after Exposure at the Cleavage Stages, Biol. Reprod., 2006, vol. 74, pp. 288–294.
Spanos, S., Rice, S., Karagiannis, P., et al., Caspase Activity and Expression of Cell Death during Development of Human Preimplantation Embryos, Reproduction, 2002, vol. 124, pp. 353–363.
Hosokawa, M., Abe, T., Higuchi, K., et al., Management and Design of the Maintenance of SAM Mouse Strains: An Animal Model for Accelerated Senescence and Age-Associated Disorders, Exptl. Gerontol., 1997, vol. 32, nos. 1–2, pp. 111–116.
Semenova, M.L., Kramarenko, G.G., Aleksandrova, A.A., et al., Na/K-ATPase Activity and Postimplantation Embryo Loss in SAMP1 Mice, Ann. Reports SAM Studies, 2001, vol. 7, nos. 12–13, pp. 107–108.
Suzuki, O., Asano, T., Yamamoto, Y., et al., Development in vitro of Preimplantation Embryos from 55 Mouse Strains, Reprod. Fertil. Dev., 1996, vol. 8, no. 6, pp. 975–980.
Kawanishi, S. and Hiraku, Y., Amplification of Anticancer Drug-Induced DNA Damage and Apoptosis by DNA-Binding Compounds, Curr. Med. Chem. Anticancer Agents, 2004, vol. 4, no. 5, pp. 415–419.
Budinger, G.R., Mutlu, G.M., Eisenbart, J., et al., Proapoptotic Bid Is Required for Pulmonary Fibrosis, Proc. Natl. Acad. Sci. USA, 2006, vol. 103, no. 12, pp. 4604–4609.
Popov, L.S. and Korochkin, L.I., Apoptosis: Genetically Programmed Cell Death, Russ. J. Genet., 2004, vol. 40, no. 2, pp. 99–113.
Sunayama, J., Ando, Y., Itoh, N., et al., Physical and Functional Interaction between BH3-Only Protein Hrk and Mitochondrial Pore-Forming Protein p32, Cell Death Differ., 2004, vol. 11, no. 7, pp. 771–781.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © M.L. Semenova, E.E. Zakharova, S.Y. Zaletov, V.V. Zaeva, N.V. Kosheleva, 2008, published in Genetika, 2008, Vol. 44, No. 9, pp. 1290–1293.
Rights and permissions
About this article
Cite this article
Semenova, M.L., Zakharova, E.E., Zaletov, S.Y. et al. The effect of antisense oligonucleoties specific to the harakiri mRNA on spontaneous and induced defects of mouse preimplantation embryo development. Russ J Genet 44, 1123–1126 (2008). https://doi.org/10.1134/S1022795408090184
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795408090184