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Specific aspects of detection of peroxidase isozymes and their genetic control in barley

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Abstract

Identical specimens were separated by electrophoresis in two gels to detect and fix peroxidase isozymes. Both gels were stained by Coomassie brilliant blue for detecting proteins. One gel was previously incubated for detecting peroxidase activity. The differences in electrophoretic patterns between the gels indicate the zones of peroxidase activity. It has been shown that locus Prx 6H, controlling a low-mobility grain peroxidase (PRX 6H), is localized to barley chromosome 6. Two loci, Alb 4H and Alb 7H, controlling the biosyntheses of water-soluble proteins of barley endosperm, were localized to chromosomes 4 and 7. It has been demonstrated that barley species is polymorphic at multiple molecular forms of peroxidase.

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Authors and Affiliations

  1. Belgorod Research Institute of Agriculture, Belgorod, 308001, Russia

    V. P. Netsvetaev

  2. Department of Botany and Methodology of Biological Teaching, Belgorod State University, Belgorod, 308015, Russia

    V. P. Netsvetaev

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  1. V. P. Netsvetaev
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Correspondence to V. P. Netsvetaev.

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Original Russian Text © V.P. Netsvetaev, 2008, published in Genetika, 2008, Vol. 44, No. 9, pp. 1287–1289.

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Netsvetaev, V.P. Specific aspects of detection of peroxidase isozymes and their genetic control in barley. Russ J Genet 44, 1121–1122 (2008). https://doi.org/10.1134/S1022795408090172

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  • DOI: https://doi.org/10.1134/S1022795408090172

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