Skip to main content
SpringerLink
Log in

Comparison of DNA sizes in a group of giant Pseudomonas aeruginosa phages by the PFGE method

  • Short Communications
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Genome sizes of Pseudomonas aeruginosa phages ϕKZ and EL earlier determined by sequence analysis were shown to correspond to sizes of their DNAs assessed by pulse-electrophoresis (PFGE). Putative “redundant” genes in ϕKZ phage genome are supposed to control functions promoting vigorous growth of the phage belonging to this species, compared to phages of EL species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Krylov, V.N. and Zhazykov, I.Zh., Pseudomonas aeruginosa Bacteriophage ϕKZ as a Probable Model for Analysis of Genetic Control of Morphogenesis, Genetika (Moscow), 1978, vol. 14, no. 4, pp. 678–685.

    CAS  Google Scholar 

  2. Smirnova, T.A., Minenkova, I.B., Khrenova, E.A., et al., Electron Microscopic Study of the Intracellular Development of the Pseudomonas aeruginosa Bacteriophage ϕKZ, Zh. Mikrobiol. Epidemiol. Immunobiol., 1983, no. 5, pp. 25–28.

  3. Khrenova, E.A., Akhverdyan, V.Z., and Krylov, V.N., Relationship of Pseudomonas aeruginosa Bacteriophages ϕKZ and 21 Possessing Unique Nucleoproteid Structure in the Head, Mol. Genet. Mikrobiol. Virusol., 1984, no. 5, pp. 31–34.

  4. Krylov, V.N., Smirnova, T.A., Rebentish, B.A., and Minenkova, I.B., Structure of ϕKZ Bacteriophage Particles, Vopr. Virusol., 1978, no. 5, pp. 568–571.

  5. Tyaglov, B.V., Krylov, V.N., Plotnikova, T.G., et al., Some Physicochemical Properties of Bacteriophage ϕKZ, Mol. Biol. (Moscow), 1980, vol. 14, no. 3, pp. 1019–1022.

    CAS  Google Scholar 

  6. Plotnikova, T.G., Dzhusupova, A.B., Khrenova, E.A., and Krylov, V.N., Genetic and Phenogenetic Study of Pseudomonas aeruginosa PAO1 Phage ϕKZ ts-Mutants, Genetika (Moscow), 1982, vol. 18, no. 11, pp. 1793–1798.

    CAS  Google Scholar 

  7. Dzhusupova, A.B., Plotnikova, T.G., and Krylov, V.N., Demonstration of Transduction of Pseudomonas aeruginosa Chromosome Markers by Virulent Bacteriophage ϕKZ in the Presence of the RMS148 Plasmid, Genetika (Moscow), 1982, vol. 18, no. 11, pp. 1799–1802.

    CAS  Google Scholar 

  8. Krylov, V.N., Smirnova, T.A., Minenkova, I.B., et al., Pseudomonas Bacteriophage ϕKZ Contains an Inner Body in Its Capsid, Can. J. Microbiol., 1984, vol. 30, pp. 758–762.

    Article  PubMed  CAS  Google Scholar 

  9. Krylov, V.N., Tolmachova, T.O., and Akhverdian, V.Z., DNA Homology in Species of Bacteriophages Active on Pseudomonas aeruginosa, Arch. Virol., 1993, vol. 131, nos. 1–2, pp. 141–151.

    Article  PubMed  CAS  Google Scholar 

  10. Burkal’tseva, M.V., Krylov, V.N., Pleteneva, E.A., et al., Phylogenetic Characterization of a Group of Giant ϕKZ-Like Bacteriophages of Pseudomonas aeruginosa, Rus. J. Genet., 2002, vol. 38, no. 11, pp. 1242–1250.

    Article  CAS  Google Scholar 

  11. Krylov, V.N., Burkal’tseva, M.V., Sykilinda, N.N., et al., Comparison of Genomes of New Gigantic Pseudomonas aeruginosa Phages from Native Populations from Different Regions, Rus. J. Genet., 2004, vol. 40, no. 4, pp. 363–368.

    Article  CAS  Google Scholar 

  12. Mesyanzhinov, V.V., Robben, J., Grymonprez, B., et al., The Genome of Bacteriophage ϕKZ of Pseudomonas aeruginosa, J. Mol. Biol., 2002, vol. 317, pp. 1–19.

    Article  PubMed  CAS  Google Scholar 

  13. Hertveldt, K., Lavigne, R., Pleteneva, E., et al., Genome Comparison of Pseudomonas aeruginosa Large Phages, J. Mol. Biol., 2005, vol. 354, no. 3, pp. 536–545.

    Article  PubMed  CAS  Google Scholar 

  14. Tetart, F., Desplates, C., Kutateladze, M., et al., Phylogeny of the Major Head and Tail Genes of the Wide-Ranging T4-Type Bacteriophages, J. Bacteriol., 2001, vol. 183, no. 1, pp. 358–366.

    Article  PubMed  CAS  Google Scholar 

  15. Adams, M.H., Bacteriophages, New York: Interscience, 1959.

    Google Scholar 

  16. Krylov, V.N., Pleteneva, E.A., Lavigne, R., et al., Comparison of the Genome for Phylogenetically Related Bacteriophages ϕKZ and EL of Pseudomonas aeruginosa: Evolutionary Aspects and Minimal Genome Size, Rus. J. Genet., 2005, vol. 41, no. 4, pp. 356–365.

    Article  CAS  Google Scholar 

  17. Burkal’tseva, M.V., Pleteneva, E.A., Shaburova, O.V., et al., Conserved Genomes of KMV-Like Bacteriorhages (T7 Supergroupp) Active on Pseudomonas aeruginosa, Rus. J. Genet., 2006, vol. 42, no. 1, pp. 27–31.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 113545, Russia

    O. V. Shaburova, E. A. Pleteneva & V. N. Krylov

  2. Division of Gene Technology, Katolieke Universitet of Leuven, Leuven, B-3001, Belgium

    K. Hertveldt

Authors
  1. O. V. Shaburova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. A. Pleteneva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Hertveldt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. N. Krylov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. Hertveldt.

Additional information

Original Russian Text © O.V. Shaburova, E.A. Pleteneva, K. Hertveldt, V.N. Krylov, 2008, published in Genetika, 2008, Vol. 44, No. 5, pp. 713–716.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shaburova, O.V., Pleteneva, E.A., Hertveldt, K. et al. Comparison of DNA sizes in a group of giant Pseudomonas aeruginosa phages by the PFGE method. Russ J Genet 44, 620–622 (2008). https://doi.org/10.1134/S1022795408050165

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795408050165

Keywords

Search

Navigation