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Genetic and cytological analyses of the male sterility mutation induced in a sorghum tissue culture with streptomycin

  • Plant Genetics
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Abstract

Treatment of sorghum callus cultures with 500–1000 mg/l streptomycin led to a high regeneration frequency of plants with complete or partial male sterility (MS), up to 100% of all green regenerants. The induced MS mutation (ms-str) was preserved in the F1 and BC1 progenies and was genetically unstable: many families produced semisterile and fertile revertants, whose progenies again contained semisterile and sterile mutants. The ms-str mutation was maintained through eight generations via selection and self-pollination of semisterile plants. The mutation was inherited as a recessive nuclear mutation in test crosses of sterile plants segregated in the progenies of fertile and semisterile revertants and was expressed only in single cases in a test cross for ms-str transfer through pollen of hybrids with restored male fertility. Recessive nuclear mutations determining a low plant height (dwarfness) and the lack of waxy bloom on the stem and leaves (bloomless) were found in male-sterile plants with the ms-str mutation. Cytological analysis of sterile plants reveal multiple abnormalities at various pollen development stages and in tapetal cells: cytomyxis, defects of chromosome conjugation, distorted cytokinesis in meiotic division II, a lack of tetrad separation, a defective formation of the microspore coat, generation of microspores with two to four nuclei, and the formation of micronuclei and large vacuoles in tapetal cells. A possible transfer of the induced cytoplasmic MS mutation into the nuclear genome and the causes of the high genetic instability are discussed.

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  1. Agricultural Research Institute of the Southeast Region, Saratov, 410010, Russia

    L. A. Elkonin & M. I. Tsvetova

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  1. L. A. Elkonin
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  2. M. I. Tsvetova
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Correspondence to L. A. Elkonin.

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Original Russian Text © L.A. Elkonin, M.I. Tsvetova, 2008, published in Genetika, 2008, Vol. 44, No. 5, pp. 663–673.

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Elkonin, L.A., Tsvetova, M.I. Genetic and cytological analyses of the male sterility mutation induced in a sorghum tissue culture with streptomycin. Russ J Genet 44, 575–583 (2008). https://doi.org/10.1134/S1022795408050104

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  • DOI: https://doi.org/10.1134/S1022795408050104

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