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Molecular analysis of some genes from plasmid p19 of the Bacillus subtilis 19 soil strain involved in conjugation

  • Molecular Genetics
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An Erratum to this article was published on 16 October 2008

Abstract

Two fragments of conjugative plasmid p19 (95 kb) from the Bacillus subtilis 19 soil strain were cloned and sequenced; these fragments carry genes, products of which are indispensable for the conjugative transfer. One of the fragments 4518 bp in size carries five open reading frames and their fragments (ORF1–ORF5). The protein corresponding to ORF4 is homologous to proteins from the family VirD4. Inactivation of ORF4 and ORF1 by insertional mutagenesis caused a three-to-fivefold decrease in the frequency of plasmid p19 conjugative transfer. Another 2932-bp fragment of p19 was shown to possess a rep region homologous to the rep region of plasmid pBS72 from the B. subtilis 72 soil strain and a novel ORF (ORF6); the protein corresponding to this ORF contains the HTH motif typical for DNA-binding proteins.

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    E. U. Poluektova, E. Yu. Gagarina, I. P. Shilovskii, E. A. Fedorina, V. Z. Nezametdinova & A. A. Prozorov

  2. Institute of Information Transmission Problems, Russian Academy of Sciences, Moscow, 127994, Russia

    S. A. Rodionova

Authors
  1. E. U. Poluektova
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  2. E. Yu. Gagarina
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  3. I. P. Shilovskii
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  4. E. A. Fedorina
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  5. V. Z. Nezametdinova
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  6. S. A. Rodionova
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  7. A. A. Prozorov
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Correspondence to E. U. Poluektova.

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Original Russian Text © E.U. Poluektova, E.Yu. Gagarina, I.P. Shilovskii, E.A. Fedorina, V.Z. Nezametdinova, S.A. Rodionova, A.A. Prozorov, 2008, published in Genetika, 2008, Vol. 44, No. 5, pp. 623–630.

An erratum to this article is available at http://dx.doi.org/10.1134/S1022795408100177.

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Poluektova, E.U., Gagarina, E.Y., Shilovskii, I.P. et al. Molecular analysis of some genes from plasmid p19 of the Bacillus subtilis 19 soil strain involved in conjugation. Russ J Genet 44, 539–545 (2008). https://doi.org/10.1134/S1022795408050050

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  • DOI: https://doi.org/10.1134/S1022795408050050

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