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Genetic variability and differentiation of three Russian populations of potato cyst nematode Globodera rostochiensis as revealed by nuclear markers

  • Molecular Genetics
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Abstract

Genetic variability of yellow potato cyst nematode G. rostochiensis from three Russian populations (Karelia, Vladimir oblast, and Moscow oblast) was investigated using two types of nuclear markers. Using RAPD markers identified with the help of six random primers (P-29, OPA-10, OPT-14, OPA-11, OPB-11, and OPH-20), it was possible to distinguish Karelian population from the group consisting of the populations from two adjacent regions (Moscow oblast and Vladimir oblast). Based on the combined matrix, containing 294 RAPD fragments, dendrogram of genetic differences was constructed, and the indices of genetic divergence and partition (P, H, and G st), as well as the gene flow indices N m between the nematode samples examined, were calculated. The dendrogram structure, genetic diversity indices, and variations of genetic distances between single individuals in each population from Karelia and Central Russia pointed to genetic isolation and higher genetic diversity of the nematodes from Karelia.

Based on polymorphism of rDNA first intergenic spacer ITS1, attribution of all populations examined to the species G. rostochiensis was proved. Small variations of the ITS1 sequence in different geographic populations of nematodes from different regions of the species world range did not allow isolation of separate groups within the species. Possible factors (including interregional transportations of seed potato) affecting nematode population structure in Russia are discussed.

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References

  1. Decker, H., Phytonematologie—Biologie und Bekampfung pflanzenparasitarer Nematoden, Berlin: Deutscher Landwirtschaftsverlag, 1969.

    Google Scholar 

  2. Bendezu, I.F., Evans, K., Burrows, P.R., et al., Inter-and Intra-Specific Variability of Potato Cyst Nematodes Globodera pallida and G. rostochiensis from Europe and South America Using RAPD-PCR, Nematologica, 1998, vol. 44, pp. 49–61.

    Article  Google Scholar 

  3. European Plant Protection Organization (EPPO) A2 List of Pests Recommended for Regulation as Quarantine Pests, Approved by Ministry of Agriculture of Russian Federation, 2003.

  4. Zinov’eva, S.V. and Udalova, Zh.V., Current Guidelines in Plant Protection against Parasitic Nematodes, Prikladnaya nematologiya (Applied Nematology), Zinov’eva, S.V. and Chizhov, V.N., Eds., Moscow: Nauka, 2006, pp. 269–291.

    Google Scholar 

  5. Da Conceicao, I.L.P.M., Dos Santos, M.C.V., de Oliveira Abrantes, I.M., and de Almeida Santos, M.S.N., Using RAPD Markers to Analyze Genetic Diversity in Portuguese Potato Cyst Nematode Populations, Nematology, 2003, vol. 5, no. 1, pp. 137–143.

    Article  Google Scholar 

  6. Roosien, J., Zandvoort, P.M., Folkertsma, R.T., et al., Single Juveniles of Potato Cyst Nematodes Globodera rostochiensis and Globodera pallida Differentiated by Randomly Amplified Polymorphic DNA, Parasitology, 1993, vol. 107, pp. 567–572.

    Article  PubMed  Google Scholar 

  7. Folkertma, R.T., Rouppe van der Voort, J.N.A.M., van Gent-Pelzer, M.P.E., et al., Inter-and Intraspecific Variation between Populations of Globodera rostochiensis and Globodera pallida Revealed by Random Amplified Polymorphic DNA, Genetics, 1994, vol. 84, pp. 807–811.

    Google Scholar 

  8. Fullaondo, A., Barrena, E., Viribay, M., et al., Identification of Potato Cyst Nematode Species Globodera rostochiensis and Globodera pallida by PCR Using Specific Primer Combinations, Nematology, 1999, vol. 1, pp. 157–163.

    Article  CAS  Google Scholar 

  9. Bulman, S.R. and Marshall, J.W., Differentiation of Australasian Potato Cyst Nematode (PCN) Populations Using the Polymerase Chain Reaction (PCR), New Zealand J. Crop and Horticult. Sci., 1997, vol. 25, pp. 123–129.

    CAS  Google Scholar 

  10. Blok, V.C., Malloch, G., Harrower, B.E., et al., Intraspecific Variation in Ribosomal DNA in Populations of the Potato Cyst Nematode Globodera pallid, J. Nematol., 1998, vol. 30, pp. 262–274.

    CAS  PubMed  Google Scholar 

  11. Subbotin, S.A., Waeyenberge, L., and Moens, M., Identification of Cyst-Forming Nematodes of the Genus Heterodera (Nematoda: Heteroderidae) Based on the Ribosomal DNA-RFLPs, Nematology, 2000, vol. 2, pp. 153–164.

    Article  CAS  Google Scholar 

  12. Sirca, S. and Urek, G., Morphometrical and Ribosomal DNA Sequence Analysis of Globodera rostochiensis and Globodera achilleae from Slovenia, Russ. J. Nematol., 2004, vol. 12, no. 2, pp. 161–168.

    Google Scholar 

  13. Semyenova, S.K., Chrisanfova, G.G., Filippova, E.K., et al., Individual and Population Variation in Cercariae of Bird Schistosomes of the Trichobilharzia ocellata Species Group as Revealed with the Polymerase Chain Reaction, Russ. J. Genet., 2005, vol. 41, no. 1, pp. 12–16.

    CAS  Google Scholar 

  14. Tamura K., Nei, M., Estimation of the Number of Nucleotide Substitutions in the Control Region of Mitochondrial DNA in Humans and Chimpanzees, Mol. Biol. Evol., 1993, vol. 10, pp. 512–526.

    PubMed  CAS  Google Scholar 

  15. Kumar, S., Tamura, K., and Nei, M., MEGA3: Integrated Software for Molecular Evolutionary Genetic Analysis and Sequence Alignment, Briefings Bioinform., 2004, vol. 5, pp. 150–163.

    Article  CAS  Google Scholar 

  16. Szalanski, A.L., Fleming, C.C., and Powers, T.O., Population Structure and Systematics of Potato Cyst Nematode Using rDNA Sequence Polymorphisms, Nematropica, 1996, vol. 26, p. 317.

    Google Scholar 

  17. Nei, M. and Li, W.H., Mathematical Model for Studying Genetic Variations in Terms of Restriction Endonucleases, Proc. Natl. Acad. Sci., 1979, vol. 76, pp. 5269–5273.

    Article  PubMed  CAS  Google Scholar 

  18. Van de Peer, Y. and de Wachter, R., TREECON for Windows: A Software Package for the Construction and Drawing of Evolutionary Trees for the Microsoft Windows Environment, Comput. Appl. Biosci., 1994, vol. 10, no. 5, pp. 569–570.

    PubMed  Google Scholar 

  19. Yeh, F.C. and Boyle, T., POPGENE Version 1.31: Microsoft Window-Based Freeware for Population Genetic Analysis, Univ. Alberta and CIFOR [http://www.ualberta.ca/~fyeh/index.htm].

  20. Wright, S., Evolution in Mendelian Populations, Genetics, 1931, vol. 16, pp. 97–159.

    PubMed  CAS  Google Scholar 

  21. Sedlak, P., Melounova, M., Skupinova, S., et al., Study of European and Czech Population of Potato Cyst Nematodes (Globodera rostochiensis and Globodera pallida) by RAPD Method, Plant Soil Environ., 2004, vol. 50, no. 2, pp. 70–74.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia

    G. G. Chrisanfova, D. A. Charchevnikov & S. K. Semyenova

  2. Center of Parasitology, Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, 119071, Russia

    I. O. Popov & S. V. Zinovieva

Authors
  1. G. G. Chrisanfova
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  2. D. A. Charchevnikov
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  3. I. O. Popov
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  4. S. V. Zinovieva
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  5. S. K. Semyenova
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Correspondence to G. G. Chrisanfova.

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Original Russian Text © G.G. Chrisanfova, D.A. Charchevnikov, I.O. Popov, S.V. Zinovieva, S.K. Semyenova, 2008, published in Genetika, 2008, Vol. 44, No. 5, pp. 616–622.

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Chrisanfova, G.G., Charchevnikov, D.A., Popov, I.O. et al. Genetic variability and differentiation of three Russian populations of potato cyst nematode Globodera rostochiensis as revealed by nuclear markers. Russ J Genet 44, 533–538 (2008). https://doi.org/10.1134/S1022795408050049

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  • DOI: https://doi.org/10.1134/S1022795408050049

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