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Isolation of trinucleotide microsatellite markers for Mystus nemurus

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Abstract

Twelve single-locus trinucleotide microsatellite markers were developed to characterize the Asian river catfish, Mystus nemurus, an important food fish in Southeast Asia. They were obtained by using a rapid method, namely, the 5′ anchored PCR enrichment protocol. The specific primers were designed to flank the repeat sequences and these were subsequently used to characterize 90 unrelated fish from Malaysia. The number of alleles per locus ranged from 2 (MnVj2-281) to 12 (MnBp8-4-43b) while the levels of heterozygosity ranged from 0.0444 (MnVj2-1-19) to 0.7458 (MnVj2-291).

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Authors and Affiliations

  1. Genetic Laboratory, Department of Biology, Faculty of Science, University Putra, Putra, 43400, Malaysia

    B. P. Hoh, S. S. Siraj & S. G. Tan

  2. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra, Putra, Malaysia

    K. Yusoff

Authors
  1. B. P. Hoh
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  2. S. S. Siraj
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  3. S. G. Tan
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  4. K. Yusoff
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Correspondence to B. P. Hoh.

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The text was submitted by the authors in English.

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Hoh, B.P., Siraj, S.S., Tan, S.G. et al. Isolation of trinucleotide microsatellite markers for Mystus nemurus . Russ J Genet 44, 369–371 (2008). https://doi.org/10.1134/S1022795408030216

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  • DOI: https://doi.org/10.1134/S1022795408030216

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