Abstract
The multilocus RAPD analysis of intergeneric, inter-and intraspecific nuclear genome polymorphism was used for the first time to assess intergeneric, interspecific, and intraspecific polymorphism in Lemnaceae growing on the territory of Russia. The origin of the chosen accessions overlapped with the natural range of duckweeds in Russia. Seventy-five Lemnaceae accessions representing eight species (L. minor, L. gibba, L. turionifera, L. japonica, L. trisulca, L. aequinoctialis, S. polyrhiza, and L. punctata) from three genera (Lemna, Spirodela, and Landoltia), were analyzed. The highest variability levels were revealed in L. minor accessions (0.03–0.20). Species L. trisulca and S. polyrhiza were characterized by values of genetic distance 0.01–0.18 and 0.03–0.16, respectively. The lowest polymorphism levels were detected for L. turionifera (0.01–0.11). The dendrogram based on RAPD data showed that L. aequinoctialis was the most genetically distant species of the genus Lemna. Accessions of species L. turionifera and L. japonica, as well as L. minor and L. gibba, did not form separate species-specific subclusters; rather, they fell into clusters with L. japonica/L. turionifera and L. minor/L. gibba. Accessions of the genera Spirodela and Landoltia formed two separate clusters combined into one group.
This is a preview of subscription content, log in via an institution to check access.
References
Stockey, R.A., Hoffman, G.L., and Rothwell, G.W., The Fossil Monocot Limnobiophyllum scutatum: Resolving the Phylogeny of Lemnaceae, Amer. J. Botany, 1997, vol. 84, no. 3, pp. 355–368.
Les, D.H., Crawford, D.J., Landolt, E., et al., Phylogeny and Systematics of Lemnaceae: the Duckweed Family, Systematic Botany, 2002, vol. 27, no. 2, pp. 221–240.
Les, D.H. and Crawford, D.J., Landoltia (Lemnaceae), a New Genus of Duckweeds, Novon, 1999, vol. 9, pp. 530–533.
Henry, R.J., Plant Genotyping: The DNA Fingerprinting of Plants, CAB Publ., 2001.
Edwards, S.K., Johonstone, C., and Thompson, C., A Simple and Rapid Method for the Preparation of Plant Genomic DNA for PCR Analysis, Nucl. Acids Res., 1991, vol. 19, no. 6, p. 1349.
Williams, J.G.K., Kubelik, A.R., Livak, K.L., et al., DNA Polymorphisms Amplified by Arbitrary Primers Are Useful as Genetic Markers, Nucl. Acids Res., 1990, vol. 18, no. 22, pp. 6531–6535.
Van de Peer, Y. and De Wachter, R., TREECON for Windows: A Software Package for the Construction and Drawing of Evolutionary Trees for the Microsoft Windows Environment, Comput. Appl. Biosci., 1994, vol. 14, pp. 569–570.
Sneath, P.H.A. and Sokal, R.R., Numerical Taxonomy—the Principles and Practice of Numerical Classification, San Francisco: Freeman, 1973.
Ryzhova, N.N., Martirosyan, L.V., Kolganova, T.V., et al., Chloroplast DNA trnL-trnF Spacer Sequences of tRNA Genes of 14 Accessions of the Family Lemnaceae, Spirodela Genus, Mol. Biol. (Moscow), 2006, vol. 40, no. 6, pp. 989–995.
Landolt, E., The Family of Lemnaceae—a Monographic Study: Biosystematic Investigations in Family of Duckweeds (Lemnaceae), Veroffentlichungen Geobotanisches Institut Rubel ETH, 1986, issue 71.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © E.V. Martirosyan, N.N. Ryzhova, K.G. Skryabin, E.Z. Kochieva, 2008, published in Genetika, 2008, Vol. 44, No. 3, pp. 417–422.
Rights and permissions
About this article
Cite this article
Martirosyan, E.V., Ryzhova, N.N., Skryabin, K.G. et al. RAPD analysis of genome polymorphism in the family Lemnaceae. Russ J Genet 44, 360–364 (2008). https://doi.org/10.1134/S1022795408030198
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795408030198