Abstract
The genotoxic effects of fungicide Conan 5FL (containing 50 g/L hexaconazole) in mouse bone-marrow cells and human lymphocytes have been evaluated. Three different concentrations of Conan 5FL (17.50, 35.0, and 70.0 μg/mL for human lymphocytes and 17.50, 35.0, and 70.0 mg/kg for mouse bone marrow cells) were studied. Conan 5FL induced significant increases (except 17.5 mg/kg for mouse bone marrow) in the frequency of chromosomal aberrations (CAs) in both test systems. This fungicide caused structural and numerical abnormalities in both mammalian cells. These are sister chromatid union, chromatid and chromosome breaks, fragments, dicentric and ring chromosomes, and polyploidy. Significant increase was found in induction and in minimum-maximum numbers of sister chromatid exchanges (SCEs) at all treatments compared with the negative control. Conan 5FL did not affect the replication index (RI) in human lymphocyte cultures, however, it significantly decreased the mitotic index (MI) in all treatment concentrations in both test systems. Using of Conan 5FL should be reconsidered due to its possible cytotoxic, clastogenic and mutagenic effects.
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Kornuta, N., Begley, E., and Nedopitanskaya, N., Genotoxic Effects of Pesticides, J. Environ. Pathol. Toxicol. Oncol., 1996, vol. 15, pp. 75–78.
Zahm, S.H., Ward, M.H., and Blair, A., Pesticides and Cancer, Occup. Med. Star, 1997, vol. 12, pp. 269–289.
Waddell, B.L., Zahm, S.H., Baris, D., et al., Agricultural Use of Organophosphate Pesticides and the Risk of Non-Hodgkin’s Lymphoma among Male Farmers (United States), Cancer Cause Control, 2001, vol. 12, 509–517.
Obe, G., Pfeiffer, P., Savage, J.R.K., et al., Chromosomal Aberrations: Formation, Identification and Distribution, Mutat. Res., 2002, vol. 504, pp. 17–36.
EC (European Commission) Eudralex: The Rules Governing Medicinal Products in the European Union, 1995, vol. 3BS6A, pp. 51–62.
Dolara, P., Salvadori, M., Capobianco, T., and Toricelli, F., Sister Chromatid Exchanges in Human Lymphocytes Induced by Dimethoate, Omethoate, Deltamethrin, Benomyl and Their Mixture, Mutat. Res. Lett., 1992, vol. 283, pp. 113–118.
Yager, J.W., Paradisin, W.M., and Rappaport, S.M., Sister Chromatid Exchanges in Lymphocytes are Increased in Relation to Longitudinally Measure Occupational Exposure to Low Concentrations of Styrene, Mutat. Res., 1993, vol. 319, pp. 155–165.
Giri, S. and Chatterjee, A., Modulation of Mitomycin C-Induced Sister Chromatid Exchanges and Cell Cycle Delay by Buthionine Sulfoximine and Reduced Glutathione in Mouse Bone Marrow Cells in vivo, Mutat. Res., 1998, vol. 413, pp. 227–234.
Shaham, J., Kaufman, Z., Gurvich, R., and Levi, Z., Frequency of Sister Chromatid Exchange among Green House Farmers Exposed to Pesticides, Mutat. Res., 2001, vol. 491, pp. 71–80.
Giri, S., Giri, A., Sharma, G.D., and Prasad, S.B., Mutagenic Effects of Carbosulfan, a Carbamate Pesticide, Mutat. Res., 2002, vol. 519, pp. 75–82.
Buchenauer, H., Mechanism of Action of Triazolyl Fungicides and Related Compounds, in Modern Selective Fungicides: Properties, Applications, Mechanism of Action, Lyr H., Ed., New York: Wiley, 1987, pp. 205–231.
Callander, R.D., An Evaluation in the Salmonella Mutagenicity Assay, Unpublished Report No. CTL/P/977 from ICI Central Toxicology Laboratory, Alderlay Park, Macclesfield, Cheshire, UK. Submitted to WHO by ICI Agrochemicals, Surray, UK, 1984. www.inchem.org/documents/jmpr/jmpmono/v90pr.htm
Callander, R.D., Hexaconazole—an Evaluation of Mutagenic Potential Using E. coli, Unpublished Report No. CTL/P/2143 from ICI Central Toxicology Laboratory, Alderlay Park, Macclesfield, Cheshire, UK. Submitted to WHO by ICI Agrochemicals, Surray, UK, 1984. www.inchem.org/documents/jmpr/jmpmono/v90pr.htm.
Sheldon, T., Howard, A.C., and Richardson, C.R., A Cytogenetic Study in Human Lymphocytes in vitro, Unpublished Report No. CTL/P/1186 from ICI Central Toxicology Laboratory, Alderlay Park, Macclesfield, Cheshire, UK. Submitted to WHO by ICI Agrochemicals, Surray, UK, 1984. www.inchem.org/documents/jmpr/jmpmono/v90pr.htm.
Trueman, R.W., Hexaconazole: Assessment for the Induction of Unscheduled DNA Synthesis in Primary Rat Hepatocyte Cultures, Unpublished Report No. CTL/P/1887 from ICI Central Toxicology Laboratory, Alderlay Park, Macclesfield, Cheshire, UK. Submitted to WHO by ICI Agrochemicals, Surray, UK, 1984. www.inchem.org/documents/jmpr/jmpmono/v90pr.htm.
Sheldon, T., Richardson, C.R., Shaw, J., and Barber, G., An Evaluation of PP523 in the Mouse Micronucleus Test, Unpublished Report No. CTL/P/1136 from ICI Central Toxicology Laboratory, Alderlay Park, Macclesfield, Cheshire, UK. Submitted to WHO by ICI Agrochemicals, Surray, UK, 1984. www.inchem.org/documents/jmpr/jmpmono/v90pr.htm.
Hext, P.M., Hexaconazole: Two Year Feeding Study in Rats, Unpublished Report No. CTL/P/1920 from ICI Central Toxicology Laboratory, Alderlay Park, Macclesfield, Cheshire, UK. Submitted to WHO by ICI Agrochemicals, Surray, UK, 1984. www.inchem.org/documents/jmpr.jmpmono/v90pr.htm.
Hext, P.M., Second Supplement to Hexaconazole: Two Year Feeding Study in Rats Measurement of Plasma Corticosterone Levels, Unpublished Report No. CTL/P/, 1920 from ICI Central Toxicology Laboratory, Alderlay Park, Macclesfield, Cheshire, UK. Submitted to WHO by ICI Agrochemicals, Surray, UK, 1984. www.inchem.org/documents/jmpr/jmpmono/v90pr.htm.
The Pesticide Manual, Tomlin, C.D.S., Ed., 2003, 13th ed., BCPC.
Speit, G. and Houpter, S., On the Mechanism of Differential Giemsa Staining of Bromodeoxyuridine Substitute Chromosomes: II. Differences between the Demonstration of Sister Chromatid Differentiation and Repalication Patterns, Hum. Gen., 1985, vol. 70, pp. 126–129.
Schneider, E.L. and Lewis, J., Aging and Sister Chromatid Exchange: VIII. Effect of the Aging Environment on Sister Chromatid Exchange Induction and Cell Cycle Kinetics in Ehrlich Ascites Tumor Cells, Mech. Ageing Dev., 1981, vol. 17, pp. 327–330.
Giri, S., Sharma, G.D., Giri, A., and Prasad, S.B., Genotoxic Effects of Malathion in Chick in Vivo Micronucleus Assay, Cytologia, 2002, vol. 67, pp.53–59.
Burim, R.V., Canalle, R., Lopes, J.L.C., et al., Genotoxic Action of the Sesquiterpene Lactone Centratherin on Mammalian Cells in vitro and in vitro, Teratogen. Carcin. Mut., 2001, vol. 21, pp. 383–393.
Natarajan, A.T., Chromosome Aberrations: Past, Present, and Future, Mutat. Res., 2002, vol. 504, pp. 3–16.
Adikari, N. and Grover, I.S., Genotoxic Effects of Some Systemic Pesticides: In Vivo Chromosomal Aberrations in Bone Marrow Cells in Rats, Environ. Mol. Mutagen., 1988, vol. 12, pp. 235–242.
Rencüzoğullari, E., Ila, H.B., Kayraldiz, A., et al., The Genotoxic Effect of the New Acaricide Etoxazole, Russ. J. Genet., 2004, vol. 40, pp. 1300–1304.
Çelik, M., Ünal, F., Yüzbaşıoğlu, D., et al., In vitro Effect of Karathane LC (Dinocap) on Human Lymphocytes, Mutagenesis, 2004, vol. 20, pp. 101–104.
Gurr, J.R., Lin, Y.C., Ho, I.C., et al., Induction of Chromatid Breaks and Tetraploidy in Chinese Hamster Ovary Cells by Treatment with Sodium Arsenite during the G2 Phase, Mutat. Res., 2002, vol. 504, pp. 135–142.
Van’t Hof, J., The Action of IAA and Kinetin on the Mitotic Cycle of Proliferative and Stationary Phase Excised Root Meristem, Exp. Cell. Res., 1968, vol. 51, pp. 167–176.
Epel, D., The Effects of Carbonmonoxide Inhibition of ATP Level and the Date of Mitosis in Sea Urching Egg, J. Cell. Biol., 1963, vol. 17, pp. 315–319.
Jain, A.K. and Andsorbhoy, R.K., Cytogenetical Studies on the Effects of some Chlorinated Pesticides: III. Concluding Remarks, Cytologia, 1988, vol. 53, pp. 427–436.
Hidalgo, A., Gonzales-Reyes, J.A., Navas, P., and Garcia-Herdugo, G., Abnormal Mitosis and Growth Inhibition in Allium cepa Roots Induced by Propham and Chlorpropham, Cytobios, 1989, vol. 57, pp. 7–14.
Tucke, J.D., Auletta, A., Cimino, M.C., et al., Sister Chromatid Exchange: Second Report of the Gene-Tox Program, Mutat. Res., 1993, vol. 297, pp. 101–180.
Soloneski, S., Gonzales, M., Piaggio, E., et al., Effect of the Dithiocarbamate Pesticide Zineb and Its Commercial Formulation Azzurro: I. Genotoxic Evaluation on Cultured Human Lymphocytes Exposed in vitro, Mutagenesis, 2001, vol. 16, pp. 487–493.
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Yilmaz, S., Aksoy, H., Ünal, F. et al. Genotoxic action of fungicide Conan 5FL (Hexaconazole) on mammalian cells in vivo and in vitro. Russ J Genet 44, 273–278 (2008). https://doi.org/10.1134/S1022795408030058
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DOI: https://doi.org/10.1134/S1022795408030058