Skip to main content
Log in

Biochemical Genetics in St. Petersburg University: From the gene-enzyme model to medical biotechnology

  • Genetics of Microorganisms
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

The history of biochemical genetics research in St. Petersburg (Leningrad) State University is described. The main research projects and achievements of the Laboratory of Biochemical Genetics in studies on the mechanisms of gene expression control, coordinated regulation of metabolism, and the relationship of the physiological state of yeast cells with the maintenance of genetic stability are discussed. The fundamental importance of studies on the acid phosphatase model for the formation and development of medical biotechnology in St. Petersburg University is demonstrated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Vatti, K.V., Ponomarenko, V.V., and Tikhomirova, M.M., Revival of Genetics in Leningrad University (1957–1970), in Issledovaniya po genetike (Research in Genetics), Leningrad: Leningr. Gos. Univ., 1974, issue 11, pp. 90–102.

    Google Scholar 

  2. Lobashev, M.E., Genetics in Leningrad University, in Issledovaniya po genetike (Research in Genetics), Leningrad: Leningr. Gos. Univ., 1967, issue 3, pp. 3–19.

    Google Scholar 

  3. Janulaitis, A.A., Nikolaeva, Z.K., Padkina, M.V., et al., The Study of Phosphoribosyl Aminoimidazole Carboxylase in Mutant Yeast Strains: II. Preparative Synthesis of 5-Aminoimidazolribotide, Vestn. Leningr. Univ., Ser. 3., 1972, no. 21, pp. 141–147.

  4. Alenin, V.V. and Domkin, V.D., Synthesis of 5-Amino-4-carboxy-1-(5′-phospho-βD-ribofuranosyl)imidazole Analogs, Zh. Obshch. Khim., 1975, vol. 45, no. 6, pp. 1405–1409.

    Google Scholar 

  5. Nikolaeva, Z.K., Alenin, V.V., Domkin, V.D., and Smirnov, M.N., The Use of Carboxyaminoimidazole Ribotide in the Determination of Phosphoribosyl Aminoimidazole Carboxylase from Saccharomyces cerevisiae, Biokhimiya (Moscow), 1975, vol. 40, no. 4, pp. 751–754.

    CAS  Google Scholar 

  6. Smirnov, M.N., Krasnopevtseva, N.G., Inge-Vechtomov, S.G., et al., The Study of Exogenous Acid Phosphatase Mutants in Yeasts Saccharomyces cerevisiae, in Issledovaniya po genetike (Research in Genetics), Leningrad: Leningr. Gos. Univ., 1974, issue 5, pp. 59–62.

    Google Scholar 

  7. Padkina, M.V., Krasnopevtseva, N.G., Petrashen’, M.G., et al., Genetic and Biochemical Study of Acid Phosphatases in Saccharomyces cerevisiae Yeast: I. Characteristic of Acid Phosphatases in Different Strains, Genetika (Moscow), 1974, vol. 10, no. 11, pp. 100–111.

    CAS  Google Scholar 

  8. Ter-Avanesian, M.D., Inge-Vechtomov, S.G., and Petrashen’, M.G., Genetic and Biochemical Study of Acid Phosphatases in Saccharomyces cerevisiae Yeast: II. Study of Mutation Affecting the Activity of Acid Phosphatase 1, Genetika (Moscow), 1974, vol. 10, no. 12, pp. 101–109.

    Google Scholar 

  9. Kozhin, S.A. and Samsonova, M.G., Genetic and Biochemical Study of Acid Phosphatases in Saccharomyces cerevisiae Yeast: IV. Genetic Control of Acid Phosphatase 2, Genetika (Moscow), 1975, vol. 11, no. 7, pp. 104–116.

    CAS  Google Scholar 

  10. Padkina, M.V., Krasnopevtseva, N.G., and Smirnov, M.N., Identification of the Acid Phosphatase 1 Structural Gene in Yeasts, in Materiala 3-go s″ezda VOGiS im. N.I. Vavilova (Proc. 3rd Conference of Vavilov All-Union Society of Geneticists and Breeders), Leningrad, 1977, p. 165.

  11. Krasnopevtseva, N.G., Urazmanova, N.A., Padkina, M.V., et al., Genetic and Biochemical Study of Acid Phosphatases in Yeast: XII. Isolations and Characterization of Repressible Acid Phosphatases in Yeasts, Vestn. Leningr. Univ., Ser. 3., 1986, no. 3, pp. 98–106.

  12. Sambuk, E.V., Pavlova, N.A., Sharypova, L.A., et al., Genetic and Biochemical Study of Acid Phosphatases in Yeast: XIV. Identification and Cloning of the ACP5 Gene, Vestn. Leningr. Univ., Ser. 3., 1988, no. 24, pp. 300–307.

  13. Padkina, M.V., Pavlova, N.A., Sambuk, E.V., et al., Analysis of the ACP5 Gene Function, in Molekulyarnye mekhanizmy geneticheskikh protesessov (Molecular Mechanisms Genetic Processes), Moscow, 1991, pp. 186–190.

  14. Sambuk, E.V., Kuchkartaev, A.I., Padkina, M.V., and Smirnov, M.N., Genetic Mapping of Genes Regulating Synthesis of Acid Phosphatases in the Yeast Saccharomyces cerevisiae of the Peterhoff Yeast Collection, Genetika (Moscow), 1991, vol. 27, no. 4, pp. 644–648.

    CAS  Google Scholar 

  15. Oshima, Y., The Phosphatase System in Saccharomyces cerevisiae, Genes Genet. Syst., 1997, vol. 72, pp. 323–334.

    Article  PubMed  CAS  Google Scholar 

  16. Koren, R., LeVitre, J., and Bostian, K.A., Isolation of the Positive-Acting Regulatory Gene PHO4 from Saccharomyces cerevisiae, Gene, 1986, vol. 41, pp. 271–275.

    Article  PubMed  CAS  Google Scholar 

  17. Sengstag, C. and Hinnen, A., The Sequence of the Saccharomyces cerevisiae Gene PHO2 Codes for Regulatory Protein with Unusual Amino Acid Composition, Nucleic Acids Res., 1987, vol. 15, pp. 233–240.

    Article  PubMed  CAS  Google Scholar 

  18. Yoshida, K., Ogawa, N., and Oshima, Y., Function of the PHO Regulatory Genes for Repressible Acid Phosphatase Synthesis in Saccharomyces cerevisiae, Mol. Gen. Genet., 1989, vol. 217, pp. 40–46.

    Article  PubMed  CAS  Google Scholar 

  19. Ueda, Y., Toh-e, A., Oshima, Y., Isolation and Characterization of Recessive, Constitutive Mutations for Repressible Acid Phosphatase Synthesis in Saccharomyces cerevisiae, J. Bacteriol., 1975, vol. 122, pp. 911–920.

    PubMed  CAS  Google Scholar 

  20. Kaffman, A., Rank, N.M., and O’shea, E.K., Phosphorylation Regulates Association of the Transcription Factor pho4 with Its Import Receptor Pse1/Kap121, Genes Dev., 1998, vol. 12, pp. 2673–2683.

    PubMed  CAS  Google Scholar 

  21. Bostian, K.A., Lemire, J.M., Cannon, L.E., et al., In vitro Synthesis of Repressible Acid Phosphatase: Identification of Multiple mRNA and Products, Proc. Natl. Acad. Sci. USA, 1980, vol. 77, pp. 4504–4508.

    Article  PubMed  CAS  Google Scholar 

  22. Schmid, A., Fascher, K.D., and Horz, W., Nucleosome Disruption at the Yeast PHO5 Promoter Upon PHO5 Induction Occurs in the Absence of DNA Replication, Cell, 1992, vol. 71, pp. 853–864.

    Article  PubMed  CAS  Google Scholar 

  23. Thevelein, J.M., Signal Transduction in Yeast, Yeast, 1994, vol. 10, pp. 1753–1790.

    Article  PubMed  CAS  Google Scholar 

  24. Waters, N.C., Knight, J.P., Creasy, C.L., et al., The Yeast Pho80-Pho85 Cyclin-CDK Complex Has Multiple Substrates, Curr. Genet., 2004, vol. 46, pp. 1–9.

    Article  PubMed  CAS  Google Scholar 

  25. Wysocki, R., Javaheri, A., Kristjansdottir, K., et al., CDK Pho85 Targets CDK Inhibitor Sic1 to Relieve Yeast G1 Checkpoint Arrest after DNA Damage, Nat. Struct. Mol. Biol., 2006, vol. 13, pp. 908–914.

    Article  PubMed  CAS  Google Scholar 

  26. Wang, Z., Wilson, W.A., Fujino, M.A., et al., Antagonistic Controls of Autophagy and Glycogen Accumulation by Snf1p, the Yeast Homolog of AMP-Activated Protein Kinase, and the Cyclin-Dependent Kinase Pho85p, Mol. Cell Biol., 2001, vol. 21, pp. 5742–5752.

    Article  PubMed  CAS  Google Scholar 

  27. Irniger, S. and Braus, G.H., Controlling Transcription by Destruction: The Regulation of Yeast Gcn4p Stability, Curr. Genet., 2003, vol. 44, pp. 8–18.

    Article  PubMed  CAS  Google Scholar 

  28. Blinnikova, E.I., Mirjuschenko, F.L., Shabalin, Y.A., et al., Vesicular Transport of Extracellular Acid Phosphatases in Yeast Saccharomyces cerevisiae, Biochemistry (Moscow), 2002, vol. 67, pp. 485–490.

    Article  CAS  Google Scholar 

  29. Popova, Yu.G., Padkina, M.V., and Sambuk, E.V., Effect of Multations in PHO85 and PHO4 Genes on Utilization of Proline in Saccharomyces cerevisiae Yeasts, Russ. J. Genet., 2000, vol. 36, no. 12, pp. 1364–1369.

    Article  CAS  Google Scholar 

  30. Padkina, M.V., Tarasov, S.A., Karsten, S.L., et al., Effect of the pho85 Mutation on the Catabolite Repression of the CIT1 Gene in Yeasts Saccharomyces cerevisiae, Russ. J. Genet., 2003, vol. 39, no. 6, pp. 604–609.

    Article  CAS  Google Scholar 

  31. Sambuk, E.V., Popova, Yu.G., and Padkina, M.V., The Study of Phosphate Effect and Mutation in PHO85, PHO80, and PHO4 Genes on the HSP82 Gene Transcription in Saccharomyces cerevisiae Yeasts, Vest. S.-Peterb. Univ., Ser. 3., 2002, no. 2, pp. 33–40.

  32. Queitsch, C., Sangster, T.A., and Lindquist, S., Hsp90 As a Capacitor of Phenotypic Variation, Nature, 2002, vol. 417, pp. 618–624.

    Article  PubMed  CAS  Google Scholar 

  33. Sambuk, E.V., Fizikova, A.Yu., Zakharova, K.V., et al., The Lack of Cyclin-Dependent Phosphoprotein Kinase Pho85p Leads to Defects in Mitochondrial Nucleoid Transmission in Yeast Saccharomyces cerevisiae, Tsitologiya, 2005, vol. 47, no. 10, pp. 917–924.

    CAS  Google Scholar 

  34. Sambuk, E.V., Popova, Yu.G., Fizikova, A.Yu., and Padkina, M.V., Genetic Analysis of Pleiotropic Effects of pho85 Mutations in Yeast Saccharomyces cerevisiae, Russ. J. Genet., 2003, vol. 39, no. 8, pp. 871–877.

    Article  CAS  Google Scholar 

  35. Miyanohara, A., Toh-e, A., Nozaki, C., et al., Expression of Hepatitis B Surface Antigen in Yeast, Proc. Natl. Acad. Sci. USA, 1983, vol. 80, pp. 1–5.

    Article  PubMed  CAS  Google Scholar 

  36. RF Patent 1 584 188, MKI S 12 N 15/00, 1988.

  37. USSR Inventor’s Certificate no. 1 530 749 A1, MKI S 12 N 15/00.

  38. RF Patent 2 180 003 S2, MKI S 12 N 15/22, 1998.

  39. RF Patent 17 703 359 S1, MKI S 12 N 15/26, 1989.

  40. RF Patent 2 230 781 S1, MKI S 12 N 15/81, 2002.

  41. RF Patnet 1 660 388 S1, MKI S 12 N 15/23, 1989.

  42. Smith, K.A., Interleukin-2: Inception, Impact, and Implications, Science, 1988, vol. 240, pp. 1169–1176.

    Article  PubMed  CAS  Google Scholar 

  43. Popovich, A.M., Interleikin-2: immunobiologiya i immunoterapiya (Interleukin-2: Immunobiology and Immunotherapy), St. Petersburg: Skif, 2004.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. V. Padkina.

Additional information

Original Russian Text © M.V. Padkina, E.V. Sambuk, 2007, published in Genetika, 2007, Vol. 43, No. 10, pp. 1358–1362.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Padkina, M.V., Sambuk, E.V. Biochemical Genetics in St. Petersburg University: From the gene-enzyme model to medical biotechnology. Russ J Genet 43, 1135–1138 (2007). https://doi.org/10.1134/S1022795407100067

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795407100067

Keywords

Navigation