Abstract
The study presents a continuation of the research aimed at producing of wheat-rye substitution lines (2n = 42) based on the cross (Triticum aestivum L. × Secale sereale L.) × Triticum aestivum L., and using winter rye cultivars Vyatka and Vietnamskaya Mestnaya. In BC 1 F 5 two lines were identified, having karyotypes in which a pair of homologous wheat chromosomes was substituted by a homeologous pair of rye chromosomes. The chromosome composition of these lines was analyzed using C-banding, GISH, and SSR markers. It was demonstrated that karyotype of each line included a single pair of rye chromosomes and lacked wheat-rye translocations. The rye chromosomes were identified, and the chromosomes of wheat, at which the substitutions occurred, were determined. The lines generated by crosses with rye of Vyatka and Vietnamskaya Mestnaya cultivars were designated 1Rv(1A) and 5Rviet(5A), respectively. Chromosome identification and classification of the lines makes it possible to use them in breeding programs and genetic studies.
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References
Friebe, B., Jiang, J., Raupp, W.J., et al., Characterization of Wheat-Alien Translocations Conferring Resistance to Diseases and Pests: Current Status, Euphytica, 1996, vol. 91, pp. 59–87.
Villareal, R.L., Banuelos, O., Mujeeb-Kazi, A., and Rajaram, S., Agronomic Performance of Chromosome 1B and T1BL.1RS Near-Isolines in the Spring Bread Wheat Seri M82, Euphytica, 1998, vol. 103, pp. 195–202.
Lukaszewski, A.J., Frequency of 1RS.1Al and 1RS.1BL Translocations in Unated States Wheats, Crop Sci., 1990, vol. 30, pp. 1151–1153.
Ko, J.M., Seo, B.B., Suh, D.Y., et al., Production of a New Wheat Line Possessing the 1BL.1RS Wheat-Rye Translocation Derived from Korean Rye Cultivar Paldanghomil, Theor. Appl. Genet., 2002, vol. 104, pp. 171–174.
Riberio-Carvalho, C., Guedes-Pinto, H., Heslop-Harrison, J.S., and Schwarzacher, T., Introgression of Rye Chromatin on Chromosome 2D in the Portuguese Wheat Landrace “Barbela”, Genome, 2001, vol. 44, pp. 1122–1128.
De Bustos, A., Rubio, P., and Jouve, N., Characterisation of Two Gene Subunits on the 1R Chromosome of Rye as Ortologs of Each of the Glu-1 Genes of Hexaploid Wheat, Theor. Appl. Genet., 2001, vol. 103, pp. 733–742.
Dundas, I.S., Frappell, D.E., Crack, D.M., and Fisher, J.M., Deletion Mapping of a Nematode Resistance Gene on Rye Chromosome 6R in Wheat, Crop Sci., 2001, vol. 41, pp. 1771–1778.
Shchapova, A. and Kravtsova, L., The Production of Wheat-Rye Substitution Lines by Using the Giemsa Staining Technique, Cereal Res. Commun., 1982, vol. 1–2, pp. 33–39.
Silkova, O.G., Dobrovol’skaya, O.B., Dubovets, N.I., et al., Production of Wheat-Rye Substitution Lines and Identification of Chromosome Composition of Karyotypes Using S-Banding, GISH, and SSR Markers, Russ. J. Genet., 2006, vol. 42, no. 6, pp. 645–653.
Badaev, N.S., Badaeva, E.D., Maximov, N.G., and Zelenin, A.V., Cytogenetic Investigation of Hybrids Produced by Crossing of Hexaploid Triticale with Common Wheats, Theor. Appl. Genet., 1985, vol. 70, pp. 536–541.
Badaeva, E.D., Sozinova, L.F., Badaev, N.S., et al., “Chromosomal Passport” of Triticum aestivum L. em Thell. Cv. Chinese Spring and Standartization of Chromosomal Analysis of Cereals, Cereal Res. Commun., 1990, vol. 18, pp. 273–281.
Schubert, I., Shi, F., Fuchs, J., and Endo, T.R., An Efficient Screening for Terminal Deletions and Translocations of Barley Chromosomes Added to Common Wheat, Plant J., 1998, vol. 14, pp. 489–495.
Silkova, O.G., Shchapova, A.I., and Shumnyi, V.K, The Role of Rye Chromosome 2R of Wheat-Rye Substitution Lines 2R(2D)1 (T. aestivum L. Cultivar Saratovskaya 29/Sesale cereale L. Cultivar Onokhoiskaya) in Genetic Regulation of Meiotic Restitution in Wheat-Rye Polyhaploids, Russ. J. Genet., 2007, vol. 43, no. 7, pp. 805–814.
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Original Russian Text © O.G. Silkova, O.B. Dobrovolskaya, N.I. Dubovets, I.G. Adonina, L.A. Kravtsova, A.I. Shchapova, V.K. Shumny, 2007, published in Genetika, 2007, Vol. 43, No. 8, pp. 1149–1152.
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Silkova, O.G., Dobrovolskaya, O.B., Dubovets, N.I. et al. Production of wheat-rye substitution lines based on winter rye cultivars with karyotype identification by means of C-banding, GISH, and SSR markers. Russ J Genet 43, 957–960 (2007). https://doi.org/10.1134/S1022795407080200
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DOI: https://doi.org/10.1134/S1022795407080200