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Proton-dependent transporter gene lndJ confers resistance to landomycin E in Streptomyces globisporus 1912

  • Genetics of Microorganisms
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Abstract

Sequence analysis of 2 kb BamHI-SmaI fragment of landomycin E (LaE) gene cluster in Streptomyces globisporus 1912 revealed one complete ORF marked as lndJ. Analysis of putative lndJ aminoacid sequence allowed us to suppose that it is proton-dependent antiporter which could be involved in resistance to LaE in the producing strain. Although disruption of lndJ had no significant influence on LaE production and resistance, its overexpression in wild type and LaE overproducing strains led to qualitative changes in landomycins biosynthesis and increased resistance to LaE. These data support the hypothesis about involvement of lndJ gene in landomycins export.

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References

  1. Mendez, C. and Salas, J.A., The Role of ABC Transporters in Antibiotic-Producing Organisms: Drug Secretion and Resistance Mechanisms, Res. Microbiol., 2001, vol. 152, pp. 341–350.

    Article  PubMed  CAS  Google Scholar 

  2. D’Costa, V.M., McGrann, K.M., Hughes, D.W., and Wright, G.D., Sampling the Antibiotic Resistome, Science, 2006, vol. 311, pp. 374–377.

    Article  PubMed  CAS  Google Scholar 

  3. Gromyko, O.M., Rebets, Y.V., Ostash, B.O., et al., Generation of Streptomyces globisporus SMY622 Strain with Increased Landomycin E Production and Its Initial Characterization, J. Antibiot., 2004, vol. 57, pp. 383–389.

    PubMed  CAS  Google Scholar 

  4. Dubyts’ka, L.P. and Fedorenko, V.O., Characteristics of Mutants of Streptomyces peucetius subsp. caesius ATCC 27952 Resistant to Anthracycline Antibiotics, Mikrobiol. Zh., 2001, vol. 63, pp. 53–61.

    CAS  Google Scholar 

  5. Rohr, J. and Thiericke, R., Angucycline Group Antibiotics, Nat. Prod. Rep., 1992, vol. 2, pp. 103–137.

    Article  Google Scholar 

  6. Depenbrock, H., Bornschlegl, S., Peter, R., et al., Assessment of Antitumor Activity of Landomycin A (NSC 6399187-A), Ann. Hematol., 1996, vol. 73, A80/316.

  7. Panchuk, R., Korynevska, A., and Stoika, R., Effect of Landomycin E on Expression of mRNA Coding for Transforming Growth Factor Beta Ligands and Specific Receptors in MCF-7 Cells, Expl. Oncol., 2005, vol. 27, pp. 330–332.

    CAS  Google Scholar 

  8. Lombo, F., Brana, A.F., Salas, J.A., and Mendez, C., Genetic Organization of the Biosynthetic Gene Cluster for the Antitumor Angucycline Oviedomycin in Streptomyces antibioticus ATCC 11891, Chembiochem, 2004, vol. 9, pp. 1181–1187.

    Article  CAS  Google Scholar 

  9. Trefzer, A., Pelzer, S., Schimana, J., et al., Biosynthetic Gene Cluster of Simocyclinone, a Natural Multihybrid Antibiotic, Antimicrob. Agents Chemother., 2002, vol. 46, pp. 1174–1182.

    Article  PubMed  CAS  Google Scholar 

  10. Decker, H. and Haag, S., Cloning and Characterization of a Polyketide Synthase Gene from Streptomyces fradiae Tu2717, Which Carries the Genes for Biosynthesis of Angucycline Antibiotic Urdamycin A and a Gene Probably Involved in Oxygenation, J. Bacteriol., 1995, vol. 21, pp. 6126–6136.

    Google Scholar 

  11. Novakova, R., Bistakova, J., Homerova, D., et al., Cloning and Characterization of a Polyketide Synthase Gene Cluster Involved in Biosynthesis of a Proposed Angucycline-Like Polyketide Auricin in Streptomyces aureofaciens CCM 3239, Gene, 2002, vol. 297, pp. 197–208.

    Article  PubMed  CAS  Google Scholar 

  12. Martin, J.F., Casqueiro, J., and Liras, P., Secretion Systems for Secondary Metabolites: How Producer Cells Send Messages of Intercellular Communication, Curr. Opin. Microbiol., 2005, vol. 8, pp. 282–293.

    Article  PubMed  CAS  Google Scholar 

  13. Zhu, L., Ostash, B., Rix, U., et al., Identification of the Function of Gene lndM2 Encoding a Bifunctional Oxygenase-Reductase Involved in the Biosynthesis of the Antitumor Antibiotic Landomycin E by Streptomyces globisporus 1912 Supports the Originally Assigned Structure for Landomycinone, J. Org. Chem., 2005, vol. 70, pp. 631–638.

    Article  PubMed  CAS  Google Scholar 

  14. Rebets, Y., Ostash, B., Luzhetskyy, A., et al., DNA-Binding Activity of Lnd I Protein and Temporal Expression of the Gene that Upregulates Landomycin E Production in Streptomyces globisporus, 1912, Microbiology, 2005, vol. 151, pp. 281–290.

    Article  PubMed  CAS  Google Scholar 

  15. Rebets, Yu., Ostash, B., Luzhetskyy, A., et al., Production of Landomycins in Strains Streptomyces globisporus 1912 and S. cyanogenus S136 is Regulated by Genes Encoding Putative Transcriptional Activators, FEMS Microbiol. Lett., 2003, vol. 222, pp. 149–153.

    Article  PubMed  CAS  Google Scholar 

  16. Ostash, B., Kozarevska, I., and Fedorenko, V., Generation of Landomycin D-Producing Strain Streptomyces globisporus LD3, Folia Microbiol., 2005, vol. 50, pp. 19–23.

    Article  CAS  Google Scholar 

  17. Ostash, B., Rix, U., Remsing-Rix, L., et al., Generation of New Landomycins by Combinatorial Biosynthetic Manipulation of the lndGT Gene of the Landomycin E Cluster in S. globisporus, Chem. Biol., 2004, vol. 11, pp. 547–555.

    Article  PubMed  CAS  Google Scholar 

  18. Kieser, T., Bibb, M.J., Buttner, M.J., et al., Practical Streptomyces Genetics, The John Innes Foundation, 2000.

  19. Luzhetskii, A., Ostash, B., and Fedorenko, V., Intergeneric Conjugation Escherichia coli-Streptomyces globisporus 1912 Using Integrative Plasmid pSET152 and Its Derivatives, Russ. J. Genet., 2001, vol. 37, pp. 1123–1129.

    Article  CAS  Google Scholar 

  20. Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Laboratory, 1989.

    Google Scholar 

  21. Bierman, M., Logan, R., O’Brien, K., et al., Plasmid Cloning Vectors for the Conjugal Transfer of DNA from Escherichia coli to Streptomyces spp., Gene, 1992, vol. 116, pp. 43–49.

    Article  PubMed  CAS  Google Scholar 

  22. Rebets, Yu., Ostash, B., Gromyko, O., et al., Resistance of Recombinant Streptomyces albus CEST 114 and Streptomyces globisporus Strains to Landomycine E, Visn, Lviv Univ. Ser. Biol., 2003, vol. 33, pp. 47–54.

    Google Scholar 

  23. Paulsen, I.T., Brown, M.H., and Skurray, R.A., Proton-Dependent Multidrug Efflux Systems, Microbiol. Rev., 1996, vol. 60, pp. 575–608.

    PubMed  CAS  Google Scholar 

  24. Westrich, L., Domann, S., Faust, B., et al., Cloning and Characterization of a Gene Cluster from Streptomyces cyanogenus S136 Probably Involved in Landomycin Biosynthesis, FEMS Microbiol. Lett., 1999, vol. 170, pp. 381–387.

    Article  PubMed  CAS  Google Scholar 

  25. Pernodet, J.L., Gourmelen, A., Blondelet-Rouault, M.H., and Cundliffe, E., Dispensable Ribosomal Resistance to Spiramycin Conferred by srmA in the Spiramycin Producer Streptomyces ambofaciens, Microbiology, 1999, vol. 145, pp. 2355–2364.

    PubMed  CAS  Google Scholar 

  26. Sletta, H., Borgos, S., Bruheim, P., et al., Nystatin Biosynthesis and Transport: nysH and nysG Genes Encoding a Putative ABC Transporter System in Streptomyces noursei ATCC 11455 are Required for Efficient Conversion of 10-Deoxynystatin, Antimicrob. Agents Chemother., 2005, vol. 49, pp. 4576–4583.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 79005, Lviv, Ukraine

    I. Ostash & V. Fedorenko

  2. Department of Microbiology and Molecular Genetics, Harvard Medical School, 02115, Boston, USA

    B. Ostash & S. Walker

Authors
  1. I. Ostash
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  2. B. Ostash
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  3. S. Walker
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  4. V. Fedorenko
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Correspondence to V. Fedorenko.

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The text was submitted by the authors in English.

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Ostash, I., Ostash, B., Walker, S. et al. Proton-dependent transporter gene lndJ confers resistance to landomycin E in Streptomyces globisporus 1912. Russ J Genet 43, 852–857 (2007). https://doi.org/10.1134/S1022795407080030

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  • DOI: https://doi.org/10.1134/S1022795407080030

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