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Suggested interrelationships of RNA-polymerase sigma S subunit and nitrogen control system in Pseudomonas chlororaphis

  • Genetics of Microorganisms
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Abstract

The effect of mutation in rpoS gene encoding sigma S subunit of RNA-polymerase on the capacity of Pseudomonas chlororaphis 449 to assimilate nitrogen was investigated. It has been shown that mutant cells with knocked-out rpoS gene had significantly lower capacity to utilize the nitrogen sources such as alanine, proline, histidine, arginine, urea, and ammonium and glutamine synthetase was downregulated in their cell free extracts. Both defects were abolished by glutamine supplementation to the medium. It is suggested that in Pseudomonas chlororaphis the association of the nitrogen control system and the system of gene expression is regulated by RNA-polymerase sigma S subunit, which can be responsible for cell adaptation at nitrogen supply limitation.

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References

  1. Loewen, P.C. and Hengge-Aronis, R., The Role of the Sigma Factor Sigma S (KatF) in Bacterial Global Regulation, Annu. Rev. Microbiol., 1994, vol. 48, pp. 53–80.

    Article  PubMed  CAS  Google Scholar 

  2. Koch, B., Worm, J., Jensen, L.E., et al., Carbon Limitation Induces Sigma(S)-Dependent Gene Expression in Pseudomonas fluorescens in Soil, Appl. Environ. Microbiol., 2001, vol. 67, pp. 3363–3370.

    Article  PubMed  CAS  Google Scholar 

  3. Kang, B.R., Cho, B.H., Anderson, A.G., and Kim, J.C., The Global Regulator GacS of Biocontrol Bacterium Pseudomonas chlororaphis O6 Regulates Transcription from the rpoS Gene Encoding a Stationary Phase Sigma Factor and Affects Survival in Oxidative Stress, Gene, 2004, vol. 325, pp. 137–143.

    Article  PubMed  CAS  Google Scholar 

  4. Magasanik, B., Reversible Phosphorylation of an Enhancer Binding Protein Regulates the Transcription of Bacterial Nitrogen Utilization Genes, Trends Biochem. Sci., 1988, vol. 13, pp. 475–479.

    Article  PubMed  CAS  Google Scholar 

  5. Magasanik, B., A Charmed Life, Annu. Rev. Microbiol., 1994, vol. 48, pp. 1–24.

    Article  PubMed  CAS  Google Scholar 

  6. Reitzer, L., Ammonia Assimilation and the Biosynthesis of Glutamine, Glutamate, Aspartate, Asparagine, l-Alanine, and d-Alanine, Escherichia coli and Salmonella: Cellular and Molecular Biology, Neidhardt, F.C., Curtiss III, Ingraham, J.L., et al., Eds., Washington: ASM Press, 2nd ed., 1996, pp. 391–407.

    Google Scholar 

  7. Johansson, C. and Gest, H., Adenylylation/Deadenylylation Control of the Glutamine Synthetase of Rhodopseudomonas capsulate, Eur. J. Biohem, 1977, vol. 81, pp. 365–371.

    Article  CAS  Google Scholar 

  8. Nyberg, K. and Clarke, P., Glutamine Synthetase Activities of Cultures of Pseudomonas aeruginosa Grown in Minimal Media with Histidine, Nitrate or Ammonium Sulfate as the Nitrogen Source, J. Gen. Microbiol., 1978, vol. 107, pp. 193–197.

    CAS  Google Scholar 

  9. Janssen, D.B., Herst, P., Joosten, H.M., and van der Drift, C., Nitrogen Control in Pseudomonas aeruginosa: A Role for Glutamine in the Regulations of the Synthesis of NADP-Dependent Glutamate Dehydrogenase, Urease and Histidase, Arch. Microbiol., 1981, vol. 128, pp. 398–402.

    Article  PubMed  CAS  Google Scholar 

  10. Janssen, D.B., Joosten, H.M., Herst, P.M., and van der Drift, C., Characterization of Glutamine-Requiring Mutants of Pseudomonas aeruginosa, J. Bacteriol., 1982, vol. 151, pp. 1176–1183.

    PubMed  CAS  Google Scholar 

  11. Cases, I., Ussery, D.W., and de Lorenzo, V., The Sigma54 Regulon (Sigmulon) of Pseudomonas putida, Environ. Microbiol., 2003, vol. 5, pp. 1281–1293.

    Article  PubMed  CAS  Google Scholar 

  12. Kohler, T., Harayama, S., Ramos, J.L., and Timmis, K.N., Involvement of Pseudomonas putida RpoN Sigma Factor in Regulation of Various Metabolic Functions, J. Bacteriol., 1989, vol. 171, pp. 4326–4333.

    PubMed  CAS  Google Scholar 

  13. Nishijyo, T., Haas, D., and Itoh, Y., The CbrA-CbrB Two-Component Regulatory System Controls the Utilisation of Multiple Carbon and Nitrogen Sources in Pseudomonas aeruginosa, Mol. Microbiol., 2001, vol. 40, pp. 917–931.

    Article  PubMed  CAS  Google Scholar 

  14. Solera, D., Arenghi, F.L., Woelk, T., et al., TouR-Mediated Effector-Independent Growth Phase-Dependent Activation of the Sigma 54 Ptou Promoter of Pseudomonas stutzeri OX1, J. Bacteriol., 2004, vol. 186, pp. 7353–7363.

    Article  PubMed  CAS  Google Scholar 

  15. Miller, J.H., Experiments in Molecular Genetics, Cold Spring Harbor: Cold Spring Harbor Lab., 1972.

    Google Scholar 

  16. Astaurova, O.B., Leonova, T.E., Polyakova, I.N., et al., Adaptation of Acrylamid Producer Rhodococcus rhodochrous M8 to Changes of Ammonia Concentration in the Medium, Prikl. Biokhim. Mikrobiol., 2000, vol. 36, pp. 21–25.

    PubMed  CAS  Google Scholar 

  17. Lapointe, J., Delcuve, G., and Duplain, L., Derepressed Level of Glutamate Synthase and Glutamine Synthetase in Escherichia coli Mutants Altered in Glutamyl-Transfer Ribonucleic Acid Synthetase, J. Bacteriol., 1975, vol. 123, pp. 843–850.

    PubMed  CAS  Google Scholar 

  18. Bender, R.A., Janssen, K.A., Resnick, A.D., et al., Biochemical Parameters of Glutamine Synthetase from Klebsiella aerogenes, J. Bacteriol., 1977, vol. 129, pp. 1001–1009.

    PubMed  CAS  Google Scholar 

  19. Lessie, T.G. and Neidhardt, F.C., Formation and Operation of the Histidine-Degrading Pathway in Pseudomonas aeruginosa, J. Bacteriol., 1967, vol. 93, pp. 1800–1810.

    PubMed  CAS  Google Scholar 

  20. Ruiz-Herrera, J. and Gonzalez, J., A Continuous Method of Urease Activity, Anal. Biochem., 1969, vol. 31, pp. 366–374.

    Article  PubMed  CAS  Google Scholar 

  21. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    PubMed  CAS  Google Scholar 

  22. Vieira, J. and Messing, J., The PUC Plasmids, an M13mp7-Derived System for Insertion Mutagenesis and Sequencing with Synthetic Universal Primers, Gene, 1982, vol. 1, pp. 259–268.

    Article  Google Scholar 

  23. Hoang, T.T., Karkhoff-Schweizer, R.A.R., Kutchma, A.J., and Schweizer, H.P., A Broad-Host-Range Flp-FRT Recombination System for Site-Specific Excision of Chromosomally-Located DNA Sequences: Application for Isolation of Unmarked Pseudomonas aeruginosa Mutants, Gene, 1998, vol. 212, pp. 77–86.

    Article  PubMed  CAS  Google Scholar 

  24. Prival, M., Brenchley, J.E., and Magasanik, B., Glutamine Synthetase and the Regulation of Histidase Formation in Klebsiella aerogenes, J. Biol. Chem., 1973, vol. 248, pp. 4334–4344.

    PubMed  CAS  Google Scholar 

  25. Janssen, D.B., Habets, A., Marugg, J.T., and van der Drift, C., Nitrogen Control in Pseudomonas aeruginosa: Mutants Affected in the Synthesis of Glutamine Synthetase, Urease, and NADP-Dependent Glutamate Dehydrogenase, J. Bacteriol., 1982, vol. 151, pp. 22–28.

    PubMed  CAS  Google Scholar 

  26. Brown, K.L. and Hughes, K.T., The Role of Anti-Sigma Factors in Gene Regulation, Mol. Microbiol., 1995, vol. 16, pp. 397–404.

    Article  PubMed  CAS  Google Scholar 

  27. Kabir, M.S., Sagara, T., Oshima, T., et al., Effect of Mutations in the rpoS Gene on Cell Viability and Global Gene Expression Under Nitrogen Starvation in Escherichia coli, Microbiology, 2004, vol. 150, pp. 2543–2553.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia

    O. B. Astaurova, I. A. Bass & I. A. Khmel

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  1. O. B. Astaurova
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  2. I. A. Bass
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  3. I. A. Khmel
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Correspondence to I. A. Khmel.

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Original Russian Text © O.B. Astaurova, I.A. Bass, I.A. Khmel, 2007, published in Genetika, 2007, Vol. 43, No. 8, pp. 1026–1031.

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Astaurova, O.B., Bass, I.A. & Khmel, I.A. Suggested interrelationships of RNA-polymerase sigma S subunit and nitrogen control system in Pseudomonas chlororaphis . Russ J Genet 43, 846–851 (2007). https://doi.org/10.1134/S1022795407080029

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  • DOI: https://doi.org/10.1134/S1022795407080029

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