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Mosaic expression pattern of the nptII gene in transgenic tobacco plants Nu 21

  • Plant Genetics
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Abstract

Mosaic expression pattern of the nptII gene in transgenic tobacco Nu 21 leaf somatic cells was demonstrated. Inheritance of this phenotype (in T1–T4 and F1 backcrosses) was revealed. Three plant groups were distinguished, with low frequency of variegation manifestation (0–21.8%), with the high frequency of mosaic progeny (63.1 to 100%), and the intermediate type, where the frequency of the appearance of mosaic plants varied in a wide range, from 0 to 100%. The data obtained suggested the existence of two metastable states of a transgene in the leaf disk somatic cells (active and silenced), which could be associated with DNA modification, i.e., methylation of cytosine within the nptII gene sequence.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    T. V. Marenkova (Novoselia), E. V. Deineko & V. K. Shumny

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  1. T. V. Marenkova (Novoselia)
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  2. E. V. Deineko
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  3. V. K. Shumny
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Original Russian Text © T.V. Marenkova (Novoselia), E.V. Deineko, V.K. Shumny, 2007, published in Genetika, 2007, Vol. 43, No. 7, pp. 943–954.

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Marenkova (Novoselia), T.V., Deineko, E.V. & Shumny, V.K. Mosaic expression pattern of the nptII gene in transgenic tobacco plants Nu 21. Russ J Genet 43, 780–790 (2007). https://doi.org/10.1134/S1022795407070101

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  • DOI: https://doi.org/10.1134/S1022795407070101

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