Abstract
Genetic variation due to heavy metal contamination has always been an interesting topic of study. Because of the numerous contaminants being found in coastal and intertidal waters, there is always much discussion and argument as to which contaminant(s) caused the variations in the genetic structures of biomonitors. This study used a Single Primer Amplification Reaction (SPAR) technique, namely Random Amplified Polymorphic DNA (RAPD), to determine the genetic diversity of the populations of the green-lipped mussel Perna viridis collected from a metal-contaminated site at Kg. Pasir Puteh and those from four relatively uncontaminated sites (reference sites). Heavy metal levels (Cd, Cu, Pb, and Zn) were also measured in the soft tissues and byssus of the mussels from all the sites. Cluster analyses employing UPGMA based on the RAPD markers grouped the populations into two major clusters; the Bagan Tiang, Pantai Lido, Pontian, and Kg. Pasir Puteh populations were in one cluster, while the Sg. Belungkor population clustered by itself. This indicated that the genetic diversity based on bands resulting from the use of all four RAPD primers on P. viridis did not indicate its potential use as a biomarker of heavy metal pollution in coastal waters. However, based on a correlation analysis between a particular metal and a band resulting from a specific RAPD primer revealed some significant (P < 0.01) correlations between the primers and the heavy metal concentrations in the byssus and soft tissues. Thus, the correlation between a particular metal and the bands resulting from the use of a specific RAPD primer on P. viridis could be used as biomonitoring tool of heavy metal pollution.
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Koehn, R.K. and Bayne, B.L., Towards a Physiological and Genetical Understanding of the Energetics of the Stress Response, Biol. J. Linnean Soc. London, 1989, vol. 37, pp. 157–171.
Hummel, H. and Patarnello, T., Genetic Effects of Pollutants on Marine and Estuarine Invertebrates, Genetics and Evolution of Aquatic Organisms, Beaumont A.R., Ed., London: Chapman and Hall, 1994, pp. 425–434.
Gillespie, R.B. and Guttman, S.I., Chemical-Induced Changes in the Genetic Structure of Populations: Effects on Allozymes, Genetics and Ecotoxicology, Forbes, V.E., Ed., New York: Taylor and Francis, 1999, pp. 55–77.
Nevo, E., Noy, R., Latvie, B., et al., Genetic Diversity and Resistance to Marine Pollution: Theory and Practice, Biol. J. Linnean Soc. London, 1986, vol. 29, pp. 139–144.
Klerks, P.L. and Weis, J.S., Genetic Adaptation to Heavy Metals in Aquatic Organisms: A Review, Environ. Pollut., 1987, vol. 45, pp. 173–205.
Ben-Shlomo, R. and Nevo, E., Isozyme Polymorphism as Monitoring of Marine Environments: The Interactive Effect of Cadmium and Mercury Pollution on the Shrimp, Palaemon elegans, Mar. Pollut. Bull., 1988, vol. 19, pp. 314–317.
Nevo, E., Molecular Evolutionary Genetics of Isozymes: Pattern, Theory and Application., Isozymes: Structure, Function and Use in Biology and Medicine, Ogita Z.I. and Markert C.L., Eds., New York: Willey-Liss, 1990, pp. 701–742.
Ma, X.L., Cowles, D.L., and Carter, R.L., Effect of Pollution on Genetic Diversity in the Bay Mussel Mytilus galloprovincialis and the Acorn Barnacles Balanus glandula, Mar. Environ. Res., 2000, vol. 50, pp. 559–563.
Moraga, D., Mdelgi-Lasram, E., Romdhane, M.S., et al., Genetic Responses to Metal Contamination in Two Clams: Ruditapes decussates and Ruditapes philippinarum, Mar. Environ. Res., 2002, vol. 54. pp. 521–525.
Mulvey, M., Newman, M.C., Vogelbein, W., and Unger, M.A., Genetic Structure of Fundulus heteroclitus from PAH-Contaminated Neighboring Sites in the Elizabeth and York Rivers, Aquat. Toxicol., 2002, vol. 61, pp. 195–209.
Ross, K., Cooper, N., Bidwell, J.R., and Elder, J., Genetic Diversity and Metal Tolerance of Two Marine Species: A Comparison Between Populations from Contaminated and Reference Sites, Mar. Pollut. Bull., 2002, vol. 44, pp. 671–679.
Sokolowski, A., Fichet, D., Garcia-Meunier, P., et al., The Relationship between Metal Concentrations and Phenotypes in the Baltic Clam Macoma balthica (L.) from the Gulf of Gdansk, Southern Baltic, Chemosphere, 2002, vol. 47, pp. 475–484.
Yap, C.K., Tan, S.G., Ismail, A., and Omar, H., Allozyme Polymorphisms and Heavy Metal Levels in the Green-Lipped Mussel Perna viridis (Linnaeus) Collected from Contaminated and Uncontaminated Sites in Malaysia, Environ. Int., 2004, vol. 30, pp. 39–46.
Bickham, J.W. and Smolen, M.J., Somatic and Heritable Effects of Environmental Genotoxins and the Emergence of Evolutionary Toxicology, Environ. Health Perspectives, 1994, vol. 102, pp. 25–28.
Bickham, J.W., Sandhu, S., Ilebert, P.D.N., et al., Effects of Chemical Contaminants on Genetic Diversity in Natural Populations: Implications for Biomionitoring and Ecotoxicology, Mutat. Res., 2000, vol. 463, pp. 33–51.
Nadig, S.G., Lee, K.L., and Adams, S.M., Evaluating Alterations of Genetic Diversity in Sunfish Populations Exposed to Contaminants Using RAPD Assay, Aquat. Toxicol., 1998, vol. 43, pp. 163–178.
Krane, D.E., Sternberg, D.C., and Burton, G.A., Randomly Amplified Polymorphic DNA Profile-Based Measures of Genetic Diversity in Crayfish Correlated with Environmental Impacts, Environ. Toxicol. Chem, 1999, vol. 18, pp. 204–208.
Yap, C.K., Tan, S.G., Ismail, A., and Omar, H., Genetic Variation of Green-Lipped Mussel Perna viridis (Linnaeus) from the West Coast of Peninsular Malaysia, Zool. Stud., 2002, vol. 41, pp. 376–387.
Jarne, P. and Lagoda, P.J.L., Microsatellites, from Molecules to Populations and back, TREE, 1996, vol. 11, pp. 424–439.
Sunnucks, P., Efficient Genetic Markers for Population Biology, Trends Ecol. Evol., 2000, vol. 15, pp. 199–203.
Yap, C.K., Ismail, A., and Tan, S.G., Background Concentrations of Cd, Cu, Pb, and Zn in the Green-Lipped Mussel Perna viridis (Linnaeus) from Peninsular Malaysia, Mar. Pollut. Bull., 2003, vol. 46, pp. 1035–1048.
Yap, C.K., Ismail, A., and Tan, S.G., Cd and Zn in the Straits of Malacca and Intertidal Sediments of the West Coast of Peninsular Malaysia, Mar. Pollut. Bull., 2003, vol. 46, pp. 1348–1353.
Siddal, S.E., A Clarification of the Genus Perna (Mytilidae), Bull. Mar. Sci., 1980, vol. 30, pp. 858–870.
Yap, C.K., Ismail, A., Tan, S.G., and Omar, H., Correlations between Speciation of Cd, Cu, Pb and Zn in Sediment and Their Concentrations in Total Soft Tissue of Green-Lipped Mussel Perna viridis from the West Coast of Peninsular Malaysia, Environ. Int., 2002, vol. 28, pp. 117–126.
Yap, C.K., Ismail, A., Tan, S.G., and Omar, H., Concentrations of Cu and Pb in the Offshore and Intertidal Sediments of the West Coast of Peninsular Malaysia, Environ. Int., 2002, vol. 28, pp. 467–479.
Winnepenninckx, B., Backeljau, T., and De Wachter, R., Extraction of High Molecular Weight DNA from Molluscs, Trends Genet., 1993, vol. 9, p. 407.
Nei, M. and Li, W.H., Mathematical Model for Studying Genetic Variations in Terms of Restriction Endonucleases, Proc. Nat. Acad. Sci. USA, 1979, vol. 76, pp. 5269–5273.
Armstrong, A., Gibbs, A., Peakell, R., and Weiler, G., RAPDistance Program, Version 1.02, Canberra: Australian National Univ., 1996.
Rohlf, F.J., NTSYS-Pc, Numerical Taxonomy and Multivariate Analysis System, Version 1.60, Exeter Software, New York, 1990.
Ong, K.L., Development of Aquaculture in Malaysia in 1980s, Kuala Lumpur: Fisheries Department, 1983.
Paran, I. and Michelmore, R.W., Development of Reliable PCR Based Markers Linked to Downy Mildew Resistance Genes in Lettuce, Theor. Appl. Genet., 1993, vol. 85, pp. 985–993.
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Yap, C.K., Chua, B.H., Teh, C.H. et al. Patterns of RAPD markers and heavy metal concentrations in Perna viridis (L.), collected from metal-contaminated and uncontaminated coastal waters: Are they correlated with each other?. Russ J Genet 43, 544–550 (2007). https://doi.org/10.1134/S1022795407050109
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DOI: https://doi.org/10.1134/S1022795407050109