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Estimation of efficiency of seed irradiation by thermal neutrons for inducing chromosomal aberration in M2 of cotton Gossypium hirsutum L.

  • Plant Genetics
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Abstract

Cytogenetic analysis of M2 plants after irradiation of cotton by thermal neutrons was performed in 56 families. In 40 plants of 27 M2 families, different abnormalities of chromosome pairing were found. These abnormalities were caused by primary monosomy, chromosomal interchange, and desynapsis. The presence of chromosome aberrations in some cases decreased meiotic index and pollen fertility. Comparison of the results of cytogenetics analysis, performed in M1 and M2 after irradiation, showed a nearly twofold decrease in the number of plants with chromosomal aberrations in M2, as well as narrowing of the spectrum of these aberrations. The latter result is explained by the fact that some mutations are impossible to detect in subsequent generations because of complete or partial sterility of aberrant M1 plants. It was established that the most efficient radiation doses for inducing chromosomal aberrations in the present study were 15 and 25 Gy, since they affected survival and fertility of altered plant to a lesser extent.

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References

  1. Sybenga, J. and Wolters, A.H.G., The Classification of the Chromosomes of Rye (Secale cereale L.): A Translocation Tester Set, Genetica, 1972, vol. 43, pp. 453–464.

    Article  Google Scholar 

  2. Gill, B.S., Burnham, C.R., Stringam, G.R., et al., Cytogenetic Analysis of Chromosomal Translocations in the Tomato: Preferential Breakage in Heterochromatin, Can. J. Genet. Cytol., 1980, vol. 22, no. 3, pp. 333–341.

    Google Scholar 

  3. Mikhailova, E.I., Sosnikhina, S.P., and Michurina, T.G., Identification of Rye Chromosomes by Means of Conjugation Test in Meiosis, Russ. J. Genet., 1993, vol. 29, no. 6, pp. 978–989.

    Google Scholar 

  4. Liu Fuzhong, Marchenko, A.N. and Gostimsky, S.A., Identification of a New Translocation in the Pea, Dokl. Akad. Nauk, 1997, vol. 357, no. 5, pp. 713–716.

    Google Scholar 

  5. Liu Fuzhong and Gostimsky, S.A., Investigation of Reciprocal Translocations in the Pea, Russ. J. Genet., 1998, vol. 34, no. 9, pp. 1069–1076.

    Google Scholar 

  6. Mahama, A.A., Deaderick, L.M., Sadanaga, K., et al., Cytogenetic Analysis of Translocations in Soybean, J. Hered., 1999, vol. 90, no. 6, pp. 648–653.

    Article  Google Scholar 

  7. Bergey, D.R., Stelly, D.M., Price, H.J., and McKnight, T.D., In Situ Hybridization of Biotinylated DNA Probes to Cotton Meiotic Chromosomes, Stain Technol., 1989, vol. 64, no. 1, pp. 25–37.

    PubMed  CAS  Google Scholar 

  8. Price, H.J., Stelly, D.M., McKnight, T.D., et al., Molecular Cytogenetic Mapping of Nucleolar Organizer Region in Cotton, J. Hered., 1990, vol. 81, no. 5, pp. 365–370.

    CAS  Google Scholar 

  9. Crane, C.F., Price, H.J., Stelly, D.M., et al., Identification of a Homoeologous Chromosome Pair by in Situ DNA Hybridization to Ribosomal RNA Loci in Meiotic Chromosomes of Cotton (Gossypium hirsutum), Genome, 1993, vol. 36, pp. 1015–1022.

    CAS  PubMed  Google Scholar 

  10. Ji, Y., De-Donato, M., Crane, C.F., et al., New Ribosomal RNA Gene Locations in Gossypium hirsutum Mapped by Meiotic FISH, Chromosoma, 1999, vol. 108, no. 3, pp. 200–207.

    Article  PubMed  CAS  Google Scholar 

  11. Brown, M.S., Identification of the Chromosomes of Gossypium hirsutum L. by Means of Translocations, J. Hered., 1980, vol. 71, pp. 266–274.

    Google Scholar 

  12. Sanamyan, M.F., Cytogenetic Effect of Cotton Seed Treatment with Thermal Neutrons, Tsitol. Genet., 2003, vol. 37, no. 3, pp. 49–54.

    Google Scholar 

  13. Sanam’yan, M.F., Evaluation of the Effect of Pollen Irradiation on Karyotype Variability in M1 Cotton Plants, Russ. J. Genet., 2003, vol. 39, no. 7, pp. 791–798.

    Article  CAS  Google Scholar 

  14. Golubovskaya, I.N., Genetic Control of Chromosome Behavior during Meiosis, in Tsitologiya i genetika meioza (Cytology and Genetics of Meiosis), Moscow: Nauka, 1975, pp. 312–343.

    Google Scholar 

  15. Sanamyan, M.F. and Sharipova, E.A., The Study of Morphologic Variability in Mutants of Cotton Gossurium hirsutum L., Proc. II Int. Conf. on Plant Anatomy and Morphology, St. Petersburg, 2002, pp. 379–380.

  16. Koduru, P.R.K. and Rao, M.K., Cytogenetics of Synaptic Mutants in Higher Plants, Theor. Appl. Genet., 1981, vol. 59, pp. 197–214.

    Google Scholar 

  17. Sanamyan, M.F. and Musæv, D.A., Detection and Cytological Study of the Aneuploid and Euploid Plants with Translocations of Chromosomes in Cotton Gossurium hirsutum L., Russ. J. Genet., 1990, vol. 26, no. 3, pp. 506–515.

    Google Scholar 

  18. Sanamyan, M.F., Petlyakova, Yu.E., and Musaev, D.A., Creation of Series of Monosomic Lines of Cotton and Their Cytogenetic Analysis, Dokl. Uzb. Akad. Nauk, 2000, no. 1, pp. 58–62.

  19. Brown, M.S., The Identification of the Individual Chromosomes of Gossurium hirsutum L., Proc. Beltwide Cotton Prod. Res. Conference, 1978, p. 70.

  20. Endrizzi, J.E, Turcotte, E.L, and Kohel, R.J, Qualitative Genetics, Cytology and Cytogenetics, In Cotton, Kohel, R.J. and Lewis, F., Eds., Madison: Am. Soc. Agron., 1984, no. 24, pp. 81–129.

    Google Scholar 

  21. Hagberg, A., Cytogenetic Analysis of Erectoides Mutations in Barley, Acta. Agr. Scand., 1954, vol. 4, pp. 472–490.

    Article  Google Scholar 

  22. Stelly, D.M., Interfacing Cytogenetics with the Cotton Genome Mapping Effort, Proc. Beltwide Cotton Prod. Res. Conference, 1993, pp. 1545–1550.

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Authors and Affiliations

  1. Ulugbek National University of Uzbekistan, Tashkent, 100174, Uzbekistan

    E. M. Rakhmatullina & M. F. Sanamyan

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  1. E. M. Rakhmatullina
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  2. M. F. Sanamyan
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Original Russian Text © E.M. Rakhmatullina, M.F. Sanamyan, 2007, published in Genetika, 2007, Vol. 43, No. 5, pp. 639–646.

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Rakhmatullina, E.M., Sanamyan, M.F. Estimation of efficiency of seed irradiation by thermal neutrons for inducing chromosomal aberration in M2 of cotton Gossypium hirsutum L.. Russ J Genet 43, 518–524 (2007). https://doi.org/10.1134/S1022795407050067

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  • DOI: https://doi.org/10.1134/S1022795407050067

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