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Russian Journal of Genetics

, Volume 43, Issue 2, pp 121–126 | Cite as

Comparative expression in Escherichia coli of the native and hybrid genes for acyl-lipid Δ9 desaturase

  • R. Maali
  • H. R. Schimschilaschvili
  • V. P. Pchelkin
  • V. D. Tsydendambaev
  • A. M. Nosov
  • D. A. Los
  • I. V. Goldenkova-Pavlova
Molecular Genetics

Abstract

Expression of the desC gene coding for acyl-lipid Δ9 desaturase of thermophilic cyanobacterium Synechocystis sp. PCC6803 was studied in Escherichia coli cells. In a hybrid gene constructed (desC-licBM3), a sequence of the native acyl-lipid Δ9 desaturase was fused in frame with the reporter gene coding for thermostable lichenase. Lichenase contained in the hybrid protein simplified selection and analysis of the expression of membrane desaturase in the heterologous host. Comparisons of the expression for the native and hybrid genes in bacterial cells showed that lichenase remained active and thermostable in the hybrid protein, while desaturase retains the capability of introducing a double bound in the corresponding position of fatty acid residues.

Keywords

Codon Usage Hybrid Gene Hybrid Protein Desaturase Gene Thermophilic Cyanobacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Pleiades Publishing, Inc. 2007

Authors and Affiliations

  • R. Maali
    • 1
    • 3
  • H. R. Schimschilaschvili
    • 1
  • V. P. Pchelkin
    • 2
  • V. D. Tsydendambaev
    • 2
  • A. M. Nosov
    • 2
    • 3
  • D. A. Los
    • 2
  • I. V. Goldenkova-Pavlova
    • 1
  1. 1.Vavilov Institute of General GeneticsRussian Academy of SciencesMoscowRussia
  2. 2.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  3. 3.Department of Plant PhysiologyMoscow State UniversityMoscowRussia

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