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Comparison of new giant bacteriophages OBP and Lu11 of soil pseudomonads with bacteriophages of the ϕKZ-supergroup of Pseudomonas aeruginosa

  • Genetics of Microorganisms
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Abstract

Study of two recently isolated giant bacteriophages Lu11 and OBP that are active on Pseudomonas putida var. Manila and Pseudomonas fluorescens, respectively, demonstrated their similarity in morphotype, genome size, and size of phage particles, with giant bacteriophages of Pseudomonas aeruginosa assigned to the supergroup of ϕKZ-like phages of the family Myoviridae. This supergroup was designated in this manner according to the best studied phage ϕKZ that belongs to the species of this group widely distributed in nature. Comparison of major polypeptide sizes of mature particles suggests similarity of certain proteins in the phages examined. In OBP particles visualized with an electron microscope, an “inner body” was detected, which points to specific DNA package intrinsic to phages of ϕKZ group. In the meantime, phages Lu11 and OBP do not exhibit resemblance among themselves or with any of earlier described ϕKZ-like phages in respect to detectable DNA homology. Note that phage Lu11 of P. putida var. Manila exhibits very slight homology with phage Lin68 of the family of P. aeruginosa ϕKZ-like phages detected only in blot hybridization. This suggests the possible involvement of these phages in interspecies recombination (“gene shuffling”) between phages of various bacterial species. Results of partial sequencing of phage genomes confirmed the phylogenetic relatedness of phage OBP to phages of the ϕKZ supergroup, whereas phage Lu11 most probably belongs to a novel species that is not a member of supergroup ϕKZ composition. The results of the study are discussed in terms of the evolution of these phages.

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Authors and Affiliations

  1. State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia

    O. V. Shaburova, S. V. Krylov, E. A. Pleteneva, M. V. Bourkaltseva & V. N. Krylov

  2. Division of Gene Technology, Katholieke Universiteit Leuven, Belgium

    K. Hertveldt, R. Lavigne & G. Volckaert

  3. Department of Biological Sciences, College of Science, University of Santo Tomas, Espana Manila, 1008, Phillippines

    D. M. A. de la Cruz

Authors
  1. O. V. Shaburova
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  2. K. Hertveldt
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  3. D. M. A. de la Cruz
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  4. S. V. Krylov
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  5. E. A. Pleteneva
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  6. M. V. Bourkaltseva
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  7. R. Lavigne
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  8. G. Volckaert
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  9. V. N. Krylov
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Additional information

Original Russian Text © O.V. Shaburova, K. Hertveldt, D.M.A. de la Cruz, S.V. Krylov, E.A. Pleteneva, M.V. Bourkaltseva, R. Lavigne, G. Volckaert, V.N. Krylov, 2006, published in Genetika, 2006, Vol. 42, No. 8, pp. 1065–1074.

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Shaburova, O.V., Hertveldt, K., de la Cruz, D.M.A. et al. Comparison of new giant bacteriophages OBP and Lu11 of soil pseudomonads with bacteriophages of the ϕKZ-supergroup of Pseudomonas aeruginosa . Russ J Genet 42, 877–885 (2006). https://doi.org/10.1134/S1022795406080059

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  • DOI: https://doi.org/10.1134/S1022795406080059

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