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The role of recombinational repair proteins in mating type switching in fission yeast cells

  • Genetics of Microorganisms
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Abstract

DNA double-strand breaks (DSBs) occur after exposing cells to ionizing radiation or under the action of various antitumor antibiotics. They can be also generated in the course cell processes, such as meiosis and mating type switching in yeast. The most preferential mechanism for the correction of DNA DSB in yeasts is recombinational repair controlled by RAD52 group genes. The role of recombinational repair in mating type switching of fission yeast cells was examined on the example of genes of this group, rhp51 + and rhp55 +. We constructed homothallic strains of genotypes h 90 rhp51 and h 90 rhp55, and found that mutant cells yielded colonies with the mottled phenotype. In addition, h 90 cells with deletions in these genes were shown to segregate heterothallic iodine-negative colonies h − and h +. The genome region, responsible for the switching process in these segregants, was analyzed by DNA hybridization. As shown in this analysis, h + segregants had the h +N or h 90 configuration of the mat region, whereas h −, the h 90 configuration. Segregants h +N contained DNA duplication in the mat region. DNA rearrangements were not detected at the mating type locus, but the level of DNA DSB formation was drastically decreased in these segregants. Thus, our results show that genes rhp51 + and rhp55 + are involved not only in the repair of induced DNA DSB, but also in the mechanism of mating type switching in fission yeast.

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References

  1. Leupold, U., Die Vererbung von homothallie und heterothallie bei Schizosaccharomyces pombe, C. R. Trav. Lab. Carlsberg Ser. Physiol., 1950, vol. 24, pp. 381–480.

    Google Scholar 

  2. Beach, D., Nurse, P., and Egel, R., Molecular Rearrangement of Mating-Type Genes in Fission Yeast, Nature, 1982, vol. 296, pp. 682–683.

    Article  PubMed  CAS  Google Scholar 

  3. Miyata, H. and Miyata, M., Modes of Conjugation in Homothallic Cells of Schizosaccharomyces pombe, J. Gen. Appl. Microbiol., 1981, vol. 27, pp. 365–371.

    Google Scholar 

  4. Arcangioli, B. and Lahondes, R., Fission Yeast Switches Mating Type by a Replication-Recombination Coupled Process, EMBO J., 2000, vol. 19, pp. 1389–1396.

    Article  PubMed  CAS  Google Scholar 

  5. Egel, R., Beach, D.H., and Klar, A.J.S., Genes Required for Initiation and Resolution Steps of Mating-Type Switching in Fission Yeast, Proc. Natl. Acad. Sci. USA, 1984, vol. 81, pp. 3481–3485.

    PubMed  CAS  Google Scholar 

  6. Gutz, H. and Schmidt, H., Switching Genes in Schizosaccharomyces pombe, Curr. Genet., 1985, vol. 9, pp. 325–331.

    Article  CAS  Google Scholar 

  7. Jia, S., Yamada, T., and Grewal, S.I., Heterochromatin Regulates Cell Type-Specific Long-Range Chromatin Interactions Essential for Directed Recombination, Cell (Cambridge, Mass.), 2004, vol. 119, pp. 469–480.

    CAS  Google Scholar 

  8. Klar, A.J. and Bonaduce, M.J., swi6, a Gene Required for Mating-Type Switching, Prohibits Meiotic Recombination in the mat2–mat3 “Cold Spot” of Fission Yeast, Genetics, 1991, vol. 129, pp. 1033–1042.

    PubMed  CAS  Google Scholar 

  9. Ostermann, K., Lorentz, A., and Schmidt, H., The Fission Yeast rad22 Gene, Having a Function in Mating-Type Switching and Repair of DNA Damages, Encodes a Protein Homolog to Rad52 of Saccharomyces cerevisiae, Nucleic Acids Res., 1993, vol. 21, pp. 5940–5944.

    PubMed  CAS  Google Scholar 

  10. Rudolph, C., Kunz, C., Parisi, S., et al., The msh2 Gene of Schizosaccharomyces pombe Is Involved in Mismatch Repair, Mating-Type Switching, and Meiotic Chromosome Organization, Mol. Cell. Biol., 1999, vol. 19, pp. 241–250.

    PubMed  CAS  Google Scholar 

  11. Khasanov, F.K. and Bashkirov, V.I., Recombinational Repair in Schizosaccharomyces pombe: Role in Maintaining Genome Integrity, Mol. Biol. (Moscow), 2001, vol. 35, no. 5, pp. 1–14.

    Google Scholar 

  12. Hartsuiker, E., Vaessen, E., Carr, A.M., and Kohli, J., Fission Yeast Rad50 Stimulates Sister Chromatid Recombination and Links Cohesion with Repair, EMBO J., 2001, vol. 20, pp. 6660–6671.

    Article  PubMed  CAS  Google Scholar 

  13. Khasanov, F.K., Salakhova, A.F., Chepurnaja, O.V., et al., Identification and Characterization of the rlp1 +, the Novel Rad51 Paralog in the Fission Yeast Schizosaccharomyces pombe, DNA Repair, 2004, vol. 3, pp. 1363–1374.

    Article  PubMed  CAS  Google Scholar 

  14. Pastink, A., Eeken, J.C.J., and Lohman, P.H.M., Genomic Integrity and the Repair of Double-Strand DNA Breaks, Mutat. Res., 2001, vol. 480, pp. 37–50.

    PubMed  Google Scholar 

  15. Ueno, M., Nakazaki, T., Akamatsu, Y., et al., Molecular Characterization of the Schizosaccharomyces pombe nbs1 + Gene Involved in DNA Repair and Telomere Maintenance, Mol. Cell. Biol., 2003, vol. 23, pp. 6553–6563.

    Article  PubMed  CAS  Google Scholar 

  16. Ogawa, T., Yu., X., Shinohara, A., and Egelman, E.H., Similarity of the Yeast Rad51 Filament to the Bacterial RecA Filament, Science, 1993, vol. 259, pp. 1896–1899.

    PubMed  CAS  Google Scholar 

  17. Sung, P., Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast Rad51 Protein, Science, 1994, vol. 265, pp. 1241–1243.

    PubMed  CAS  Google Scholar 

  18. Sung, P. and Robberson, D.L., DNA Strand Exchange Mediated by a Rad51-ssDNA Nucleoprotein Filament with Polarity Opposite to That of RecA, Cell (Cambridge, Mass.), 1995, vol. 82, pp. 453–461.

    CAS  Google Scholar 

  19. Sung, P., Yeast Rad55 and Rad57 Proteins Form a Heterodimer that Functions with Replication Protein A to Promote DNA Strand Exchange by Rad51 Recombinase, Genes Dev., 1997, vol. 11, pp. 1111–1121.

    PubMed  CAS  Google Scholar 

  20. Alfa, C., Experiments with Fission Yeast, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1993.

    Google Scholar 

  21. Gutz, H., Schizosaccharomyces pombe, Handbook of Genetics, King, R.C., Ed., New York: Plenum, 1974, pp. 395–446.

    Google Scholar 

  22. Leupold, U., Methodishes zur Genetik von Schizosaccharomyces pombe, Schweiz. Z. Pathol. Bakteriol., 1955, vol. 18, pp. 1141–1146.

    PubMed  CAS  Google Scholar 

  23. Wright, A.P.H., Maundrell, K., and Shall, S., Transformation of Schizosaccharomyces pombe by Non-Homologous Unstable Integration of Plasmids in the Genome, Curr. Genet., 1986, vol. 10, pp. 503–508.

    Article  PubMed  CAS  Google Scholar 

  24. Prozorov, A.A., Bashkirov, V.I., Khasanov, F.K., et al., Insertion of Eukaryotic DNA into the Bacillus subtilis Genome by Means of a Temperature-Sensitive Plasmid Vector, Gene, 1985, vol. 34, pp. 39–46.

    Article  PubMed  CAS  Google Scholar 

  25. Fleck, O., Rudolph, C., Albrecht, A., et al., The Mutator Gene swi8 Effects Specific Mutations in the Mating-Type Region of Schizosaccharomyces pombe, Genetics, 1994, vol. 138, pp. 621–632.

    PubMed  CAS  Google Scholar 

  26. Nielsen, O. and Egel, R., Mapping the Double-Strand Break at the Mating-Type Locus in Fission Yeast by Genomic Sequencing, EMBO J., 1989, vol. 8, pp. 269–276.

    PubMed  CAS  Google Scholar 

  27. Fleck, O., Heim, L., and Gutz, H., A Mutated swi4 + Gene Causes Duplication in the Mating-Type Region of Schizosaccharomyces pombe, Curr. Genet., 1990, vol. 18, pp. 501–509.

    Article  PubMed  CAS  Google Scholar 

  28. Hays, S.L., Firmenich, A.A., and Berg, P., Complex Formation in Yeast Double-Strand Break Repair: Participation of Rad51, Rad52, Rad55, and Rad57 Proteins, Proc. Natl. Acad. Sci. USA, 1995, vol. 92, pp. 6925–6929.

    PubMed  CAS  Google Scholar 

  29. Johnson, R.D. and Symington, L.S., Functional Differences and Interactions among the Putative RecA Homologs RAD51, RAD55, and RAD57, Mol. Cell. Biol., 1995, vol. 15, pp. 4843–4850.

    PubMed  CAS  Google Scholar 

  30. Khasanov, F.K., Savchenko, G.V., Bashkirova, E.V., et al., A New Recombinational DNA Repair Gene from Schizosaccharomyces pombe with Homology to Escherichia coli RecA, Genetics, 1999, vol. 152, pp. 1557–1572.

    PubMed  CAS  Google Scholar 

  31. Lovett, S.T. and Mortimer, R.K., Characterization of Null Mutants of the RAD55 Gene of Saccharomyces cerevisiae: Effects of Temperature, Osmotic Strength and Mating Type, Genetics, 1987, vol. 116, pp. 547–553.

    PubMed  CAS  Google Scholar 

  32. Tsutsui, Y., Khasanov, F.K., Shinagawa, H., et al., Multiple Interactions among the Components of the Recombinational DNA Repair System in Schizosaccharomyces pombe, Genetics, 2001, vol. 159, pp. 91–105.

    PubMed  CAS  Google Scholar 

  33. Kim, W.J., Lee, S., Park, M.S., et al., Rad22 Protein, a Rad52 Homologue in Schizosaccharomyces pombe, Binds to DNA Double-Strand Breaks, J. Biol. Chem., 2000, vol. 10, pp. 35607–35611.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia

    D. A. Vagin, F. K. Khasanov & V. I. Bashkirov

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  1. D. A. Vagin
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  2. F. K. Khasanov
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  3. V. I. Bashkirov
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Original Russian Text © D.A. Vagin, F.K. Khasanov, V.I. Bashkirov, 2006, published in Genetika, 2006, Vol. 42, No. 4, pp. 487–493.

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Vagin, D.A., Khasanov, F.K. & Bashkirov, V.I. The role of recombinational repair proteins in mating type switching in fission yeast cells. Russ J Genet 42, 385–391 (2006). https://doi.org/10.1134/S1022795406040041

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