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Molecular evidence for the lysogenic state of microorganisms belonging to the genus Bordetella and characterization of Bordetella parapertussis temperate bacteriophage 662-2

  • Genetics of Microorganisms
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Abstract

A new bacteriophage ϕK of microorganisms belonging to the genus Bordetella was isolated from cells of the earlier characterized strains 662-2 (1 and 2) obtained upon phage conversion of B. parapertussis 17 903 cells by B. pertussis bacteriophage ϕ134. Bacteriophage ϕK is identical to previously described Bordetella bacteriophages ϕT, ϕ134, and ϕ214 in morphology and some biological properties but has a permuted genome different from all other phages. DNA of bacteriophage ϕK is not integrated in the chromosome of B. parapertussis 17 903, similar to DNA of bacteriophages ϕT, ϕ134, and ϕ214 that are not integrated into B. pertussis and B. bronchiseptica chromosomes, but may be present in a small part of the bacterial population as linear plasmids. Sequences homologous to DNA of bacteriophage ϕK were detected in the chromosome of strain 662-2 (1 and 2) and in chromosomes of all tested strains B. pertussis and B. bronchiseptica. Prophage integration in chromosomes of microorganisms of the genus Bordetella may vary in different bacterial strains and species. An assumption about abortive lysogeny of B. parapertussis bacteria for ϕK phage and of B. pertussis and B. bronchiseptica for closely related phages ϕT, ϕ134, and ϕ214 has been advanced. The possibility of involvement of B. pertussis insertion sequences in the formation of the chromosomal structure in 662-2 convertants and in phage genomes is considered.

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Authors and Affiliations

  1. Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, 123098, Russia

    L. N. Sinyashina & G. I. Karataev

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  1. L. N. Sinyashina
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  2. G. I. Karataev
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Original Russian Text © L.N. Sinyashina, G.I. Karataev, 2006, published in Genetika, 2006, Vol. 42, No. 3, pp. 339–348.

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Sinyashina, L.N., Karataev, G.I. Molecular evidence for the lysogenic state of microorganisms belonging to the genus Bordetella and characterization of Bordetella parapertussis temperate bacteriophage 662-2 . Russ J Genet 42, 259–267 (2006). https://doi.org/10.1134/S1022795406030057

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  • DOI: https://doi.org/10.1134/S1022795406030057

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