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Retroviral-mediated gene transfer as an effective tool for the in vitro genetic transformation of chicken embryonic cells and production of transgenic chickens

  • Animal Genetics
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Abstract

The data on the in vitro and in vivo (into embryonic disk) retroviral-mediated transfer of genetic information into chicken embryonic cells are presented. The estimated transformation frequency of the cultured target cells constituted 8 × 10−4 to 5 × 10−3. A transgenic rooster, carrying recombinant DNA in blood, heart, liver, and intestine cells, was obtained.

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Original Russian Text © N.A. Volkova, N.A. Zinovieva, L.A. Volkova, L.K. Emst, 2006, published in Genetika, 2006, Vol. 42, No. 1, pp. 84–88.

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Volkova, N.A., Zinovieva, N.A., Volkova, L.A. et al. Retroviral-mediated gene transfer as an effective tool for the in vitro genetic transformation of chicken embryonic cells and production of transgenic chickens. Russ J Genet 42, 72–75 (2006). https://doi.org/10.1134/S1022795406010108

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  • DOI: https://doi.org/10.1134/S1022795406010108

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