Abstract
The T7-like ΦKMV bacteriophage active on Pseudomonas aeruginosa was previously isolated by us and shown to have DNA resistant to many endonucleases. A loss of sensitive sites might be a consequence of a long ΦKMV evolution on different hosts. To elucidate, whether this trait is shared by other similar phages, several new ΦKMV-like phages were isolated from different sources and compared. All studied ΦKMV-like phages formed three groups, insignificantly differing in the number and localization of endonuclease-sensitive DNA sites. This confirms that the present-day phages of this species have highly conserved genomes. Mutational “restoration” of the lost sites may be restricted by a lethal effect. The ΦKMV-like phages were shown for the first time to increase the rate of in vitro accumulation of giant KZ-like phages of P. aeruginosa. This effect is characteristic only of ΦKMV-like phages.
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Original Russian Text © M.V. Burkaltseva, E.A. Pleteneva, O.V. Shaburova, V.A. Kadykov, V.N. Krylov, 2006, published in Genetika, 2006, Vol. 42, No. 1, pp. 33–38.
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Burkaltseva, M.V., Pleteneva, E.A., Shaburova, O.V. et al. Conserved genomes of ΦKMV-like bacteriophages (T7 supergroup) active on Pseudomonas aeruginosa . Russ J Genet 42, 27–31 (2006). https://doi.org/10.1134/S1022795406010030
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DOI: https://doi.org/10.1134/S1022795406010030