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Application of Bromocresol Green for Spectrophotometric Determination of Alkaloid Content Using the Example of Ruta graveolens

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Abstract

Studies were carried out to select conditions for the spectrophotometric determination of total alkaloid content using bromocresol green (BCG): buffer pH, formation time of the BCG-alkaloid ion pair, and stability time of the BCG-alkaloid ion pair. It is shown that the method is applicable for tropane, isoquinoline, indole, and pyridine alkaloids. The method was used for rapid assessment of alkaloid content in the biomass of Ruta graveolens L. regenerated plants and in the pharmaceutical preparation Ruta graveolens herb. Using a minimum volume of dry plant material (20 mg), it was determined that the content of alkaloids in regenerated plants was 10.55 mg/g dry weight, which is 1.62 times less than in pharmaceutical drug samples. Using reverse-phase HPLC, 16 compounds of varying intensity were identified in purified alkaloid fractions, six of which were common to regenerating plants and to the pharmaceutical preparation.

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Funding

The study was carried out with the financial support of the Ministry of Science and Higher Education of the Russian Federation within the framework of state assignments carried out by the Kazan Institute of Biochemistry and Biophysics, Federal Research Center Kazan Scientific Center, Russian Academy of Sciences (state registration no. 122011800137-0).

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  1. Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russia

    A. I. Valieva & A. N. Akulov

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Correspondence to A. I. Valieva.

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Abbreviations: BCG—bromocresol green; ultrasound—U/S.

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Valieva, A.I., Akulov, A.N. Application of Bromocresol Green for Spectrophotometric Determination of Alkaloid Content Using the Example of Ruta graveolens. Russ J Plant Physiol 71, 33 (2024). https://doi.org/10.1134/S1021443724604336

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