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Scutellaria baicalensis Georgi: Projection of Root Metabolome on Hairy Root Culture

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Abstract

The study characterizes flavonoid and phenylethanoid metabolites of Baikal skullcap (Scutellaria baicalensis). The roots of intact plants and the hairy root cultures were compared. In the culture derived from seeds of wild plants, almost the same set of metabolites as in the whole root was found. Meanwhile, the levels of methylated and glycosylated derivatives of both classes of substances were different. The roots of intact plants accumulated significant amounts of sucrose. Methylated flavones, responsible for plant protection from biotic and abiotic stress-factors, were more abundant in the hairy root cultures. In both specimens, 7‑sulfate 6-ОМе wogonin was identified for the first time. It is concluded that visualization of mass-spectrometric data of a metabolome represents a convenient tool to reveal changes in the metabolome caused by environmental factors or plant damage.

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ACKNOWLEDGMENTS

We thank Prof. A.M. Nosov for the proposition to compare metabolome profiles of the plant’s root and hairy root culture.

Funding

The work was supported by the Ministry of Education and Science of Russian Federation through State Tasks nos. 121031000120-9 and 121050500047-5.

Author information

Authors and Affiliations

  1. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 690022, Vladivostok, Russia

    Yu. N. Elkin

  2. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, 690022, Vladivostok, Russia

    A. Yu. Manyakhin

  3. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276, Moscow, Russia

    A. Yu. Stepanova

Authors
  1. Yu. N. Elkin
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  2. A. Yu. Manyakhin
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  3. A. Yu. Stepanova
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Corresponding author

Correspondence to A. Yu. Stepanova.

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The authors of this work declare that they have no conflicts of interest.

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The work is dedicated to Inna N. Kuzovkina

Translated by A. Aver’yanov

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Abbreviations: IMS—integral mass-spectrum; LC–MS—liquid chromatography–mass spectrometry tandem; MF—methylated flavones; PE—phenylethanoids; TIC—total ion current.

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Elkin, Y.N., Manyakhin, A.Y. & Stepanova, A.Y. Scutellaria baicalensis Georgi: Projection of Root Metabolome on Hairy Root Culture. Russ J Plant Physiol 70, 171 (2023). https://doi.org/10.1134/S1021443723603166

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  • DOI: https://doi.org/10.1134/S1021443723603166

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