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Sterol Composition of Lichen Peltigera canina When Exposed to Unfavorable Temperatures

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Abstract

Currently, special attention is paid to the study of the mechanisms of stress resistance of extremophile organisms that can survive in extreme conditions. Such organisms include lichens, which are symbiotic associations of fungi and algae and/or cyanobacteria. The high stress resistance of lichens is due to the presence of a wide range of biologically active metabolites, including sterols. It is known that lichens have a diverse and unique sterol composition, different from that of fungi and algae. Sterol-mediated biochemical mechanisms of stress resistance in lichens have not been fully studied and not systematized. Temperature stress is quite common for lichens, which often grow in unfavorable conditions. It is known that dry lichen thalli are able to withstand temperature changes over large ranges, while hydrated thalli are much more sensitive to unfavorable temperatures. In this work, stress-induced changes in respiratory activity and membrane stability index (MSI), as well as the sterol profile of hydrated lichen thalli, of Peltigera canina (L.) Willd. under the influence of elevated (+40°С) and low (–20°С) temperatures was investigated. It was shown that unfavorable temperatures caused a suppression of respiration rate and a decrease in the MSI of lichen thalli. Chromatomass spectrometric analysis showed the presence of P. canina ergosterol, dehydroergosterol, episterol, lichesterol, and fungisterol. Under the influence of both stress factors, there was a decrease in the level of ergosterol and an increase in the proportion of episterol. Under cold stress conditions, the proportion of dehydroergosterol also increased, the proportion of lichesterol decreased, and the relative content of the more saturated sterol fungisterol remained at the control level. It can be assumed that stress-induced changes in the sterol profile of lichens under low-temperature exposure create an optimal balance of sterols in membranes, which provides conditions for the deployment of a successful strategy leading to the adaptation of the lichen to the action of a stressor.

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Funding

The work was carried out within the framework of the state assignment of the Kazan Scientific Center of the Russian Academy of Sciences (assessment of respiratory activity and MSI), as well as with the financial support of a grant from the Russian Scientific Fund https://rscf.ru/project/22-14-00362 (manager) Yu.N. Valitova).

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Authors and Affiliations

  1. Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russia

    J. N. Valitova, V. R. Khabibrakhmanova, V. L. Uvaeva, A. F. Khairullina, D. F. Rakhmatullina, E. I. Galeeva, M. A. Swid & F. V. Minibayeva

  2. Kazan National Research Technological University, Kazan, Russia

    V. R. Khabibrakhmanova

  3. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russia

    V. M. Babaev

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  1. J. N. Valitova
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Abbreviations: MSI—membrane stability index; LPC—lysophosphatidylcholine; PI—phosphatidylinositol; PA—phosphatidic acid; PC—phosphatidylcholine; PE—phosphatidylethanolamine.

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Valitova, J.N., Khabibrakhmanova, V.R., Babaev, V.M. et al. Sterol Composition of Lichen Peltigera canina When Exposed to Unfavorable Temperatures. Russ J Plant Physiol 70, 180 (2023). https://doi.org/10.1134/S1021443723603154

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