Abstract
Callus cultures and microclones of sweet basil (Ocimum basilicum L.) were obtained in vitro and their growth and biochemical characteristics were studied depending on the hormonal composition of the MS medium as well as on the addition of zinc ferrate nanoparticles (NPs) to the nutrient medium. During clonal micropropagation of samples, the advantage of various options for the composition of nutrient media was noted: adding NAA to the MS medium for the cultivars Lyubimchik and Vasilisk, IBA for the cultivar Fioletovy barkhat and the species sample from Germany, and IAA for species samples from Poland and Italy. It should be noted that plants of the purple-leaved cultivar Fioletovy barkhat preferred MS medium containing mineral salts at a concentration of ½ normal. The results of the study confirmed the hypothesis put forward by various authors about the ability of microplants and basil callus cells to accumulate secondary metabolites as well as the possibility of controlling this process with the help of biological (mineral and hormonal composition of the nutrient medium) and physical (NP) elicitors. It was shown that the presence of zinc ferrate NPs in the MS medium contributed to the formation of callus tissue of different types of density and color. The addition of 25 μg/L NPs to the MS medium significantly increased the fresh biomass of callus tissue compared to other experimental options. In this variant, the growth index of callus tissue was the highest and amounted to 3.55. When the concentration of NPs increased to 50 μg/L, a decrease in the growth index was noted, which indicates their inhibitory effect on the proliferative activity of dedifferentiated cells. A comprehensive analysis of phenolic compounds was carried out in the resulting cell cultures. It was revealed that the accumulation of the total phenolics and flavonoids does not depend on the concentration of NPs in the MS medium.
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REFERENCES
World Flora Online (WFO), http://www.worldfloraonline.org. Accessed August 29, 2023.
Sevruk, I.A., Pisarev, D.I., Novikov, O.O., Alekseeva, K.A., and Malyutina, A.Yu., Study of the composition of the essential oil of common basil—Ocimum basilicum L. flora of the Belgorod region, Nauch. rez. biomed. issl., 2015, vol. 1, no. 3, p. 97. https://doi.org/10.18413/2313-8955-2015-1-3-97-103
Pisarev, D.I., Alekseeva, K.A., Novikov, O.O., Kornienko, I.V., and Sevruk, I.A., Chemical study of the composition of grass Ocimum basilicum L. anthocyanins, Nauch. rez. biomed. issl., 2015, vol. 1, no. 4, p. 119. https://doi.org/10.18413/2313-8955-2015-1-4-119-124
Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tabacco tissue cultures, Physiol. Plant., 1962, vol. 15, p. 473. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Aiyegoro, O.A. and Okoh, A.I., Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC, BMC Complemen. Altern. Med., 2010, vol. 10, p. 1. https://doi.org/10.1186/1472-6882-10-21
Baghel, P.S. and Ray, S., Preliminary phytochemical screening of certain aphrodisiac plants used in traditional system of medicine, Int. J. Botany Stud., 2017, vol. 2, p. 33.
Sreevidya, N. and Mehrotra, S., Spectrophotometric method for estimation of alkaloids precipitable with Dragendorff’s reagent in plant materials, J. AOAC Int., 2003, vol. 86, p. 1124. https://doi.org/10.1093/jaoac/86.6.1124
Obianime, A.W. and Uche, F.I., The phytochemical screening and effects of methanolic extract of Phyllanthus amarus leaf on the biochemical parameters of male guinea pigs, J. Appl. Sci. Environ. Manage., 2008, vol. 12, p. 73. https://doi.org/10.4314/jasem.v11i4.55199
Ruthiran, P. and Selvaraj, C.I., Phytochemical screening and in vitro antioxidant activity of Parkia timoriana (DC.) Merr., Res. J. Biotech., 2017, vol. 12, p. 47.
Singleton, V.L. and Rossi, J.A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Am. J. Enol. Vitic., 1965, vol. 16, p. 144. https://doi.org/10.5344/ajev.1965.16.3.144
Zaprometov, M.N., Phenolic compounds and methods for their research, In: Biochemical methods in plant physiology, Pavlinova, O.A., Ed., M.: Nauka, 1971, p. 185.
Chang, C., Yang, M. and Chern, J., Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J. Food Drug Anal., 2002, vol. 10, p. 178. https://doi.org/10.38212/2224-6614.2748
Stanojević, L., Stanković, M., Nikolić, V., Nikolić, L., Ristić, D., Čanadanovic-Brunet, J., and Tumbas, V., Antioxidant activity and total phenolic and flavonoid contents of Hieracium pilosella L. extracts, Sensors, 2009, vol. 9, p. 5702. https://doi.org/10.3390/s90705702
Butenko, R.G., Culture of isolated tissues and physiology of plant morphogenesis, M.: Nauka, 1964.
Butenko, R.G., Cellular and molecular aspects of plant morphogenesis in vitro, I Chailakhyanov Readings, Pushchino: Pushchinsky SC, 1994, p. 7.
Butenko, R.G., Biology of higher plant cells in vitro and biotechnology based on them, M.: FBK-PRESS, 1999.
Nosov, A.M., Functions of secondary plant metabolites in vivo and in vitro, Rus. J. Plant Physiol., 1994, vol. 41, p. 873.
Nosov, A.M., Cell culture of higher plants - a unique system, model, tool, Rus. J. Plant Physiol., 1999, vol. 46, p. 837.
Nosov, A.M., Application of cell technologies for production of plant-derived bioactive substances of plant origin, Appl. Biochem. Microbiol., 2012, vol. 48, p. 609. https://doi.org/10.1134/S000368381107009X
Kumar, D.R. and Kumar, S.A., Plant biotechnology: importance of plant tissue culture, applications and advantages, Eur. Academic Res., 2015, vol. 6, p. 6134.
Siddique, I. and Anis, M., An improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L., Acta Physiol. Plant., 2008, vol. 30, p. 493. https://doi.org/10.1007/s11738-008-0146-6
Shahzad, A., Faisal, M., Ahmad, N., Anis, M., Alatar, A., and Hend, A.A., An efficient system for in vitro multiplication of Ocimum basilicum through node culture, Afr. J. Biotechnol., 2012, vol. 11, p. 6055. https://doi.org/10.5897/AJB12.154
Verma, S.K., Sahin, G., Das, A.K., and Gurel, E., In vitro plant regeneration of Ocimum basilicum L. is accelerated by zinc sulfate, In Vitro Cell. Dev. Biol. Plant, 2016, vol. 52, p. 20. https://doi.org/10.1007/s11627-015-9739-0
Dode, L.B., Bobrowski, V.L., Bolacel Braga, E.J., Kömmling Seixas, F., and Wulff Schuch, M., In vitro propagation of Ocimum basilicum L. (Lamiaceae), Acta Sci. Biol. Sci., 2003, vol. 25, p. 435.
Begum, F., Amin, M.N., and Azad, M.A.K., In vitro rapid clonal propagation of Ocimum basilicum L., Plant Tissue Cult. Biotechnol., 2002, vol. 12, p. 27.
González-Sánchez, M.I., Lee, P.T., Guy, R.H., and Compton, R.G., In situ detection of salicylate in Ocimum basilicum plant leaves via reverse iontophoresis, Chem. Commun., 2015, vol. 51, p. 16534. https://doi.org/10.1039/C5CC06909B
Tuzhikova, M.O., Assessment of the growth of callus culture of common basil, In: Current problems of biomedicine - 2021: Proceedings of the XXVII All-Russian Conference of Young Scientists with International Participation, St. Petersburg,2021, p. 287.
Bhuvaneshwari, K., Sathya, B., Gokulanathan, A., Jayanthi, M., and Girija, S., Induction of in vitro roots from leaf callus of Ocimum basilicum L. and O. tenuiflorum L., Plant Cell Biotechnol. Mol. Biol., 2012, vol. 13, p. 15. https://doi.org/10.1016/j.foodchem.2015.07.136
Jiang, X., Liu, Y., Li, W., Zhao, L., Meng, F., Wang, Y., Tan, H., Yang, H., Wei, C., Wan, X., and Gao, L., Tissue-specific, development-dependent phenolic compounds accumulation profile and gene expression pattern in tea plant [Camellia sinensis], PLoS ONE, 2013, vol. 8, p. e62315. https://doi.org/10.1371/journal.pone.0062315
Bhuvaneshwari, K., Gokulanathan, A., Jayanthi, M., Govindasamy, V., Milella, L., Lee, S., Yang, D.C., and Girija, S., Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?, Food Chem., 2016, vol. 194, p. 55. https://doi.org/10.1016/j.foodchem.2015.07.136
Nazir, M., Tungmunnithum, D., Bose, S., Drouet, S., Garros, L., Giglioli-Guivarc’h, N., Abbasi, B.H., and Hano, C., Differential production of phenylpropanoid metabolites in callus cultures of Ocimum basilicum L. with distinct in vitro antioxidant activities and in vivo protective effects against UV stress, J. Agric Food Chem., 2019, vol. 67, p. 1847. https://doi.org/10.1021/acs.jafc.8b05647
Sanni, S., Onyeyili, P.A., and Sanni, F.S., Phytochemical analysis, elemental determination and some in vitro antibacterial activity of Ocimum basilicum L. leaf extracts, Res. J. Phytochem., 2008, vol. 2, p. 77.
Khair-ul-Bariyah, S., Ahmed, D., and Ikram, M., Ocimum basilicum: a review on phytochemical and pharmacological studies, Pak. J. Chem., 2012, vol. 2, p. 78. https://doi.org/10.15228/2012.v02.i02.p05
El-Beshbishy, H.A. and Bahashwan, S.A., Hypoglycemic effect of basil (Ocimum basilicum) aqueous extract is mediated through inhibition of α-glucosidase and α‑amylase activities: an in vitro study, Toxicol. Ind. Health, 2012, vol. 28, p. 42. https://doi.org/10.1177/0748233711403193
Akoto, C.O., Acheampong, A., Boakye, Y.D., Naazo, A.A., and Adomah, D.H., Anti-inflammatory, antioxidant, and anthelmintic activities of Ocimum basilicum (Sweet Basil) fruits, J. Chem., 2020, vol. 2020, p. 2153534. https://doi.org/10.1155/2020/2153534
Marwat, S.K., Khan, M.S., Ghulam, S., Anwar, N., Mustafa, G., and Usman, K., Phytochemical constituents and pharmacological activities of sweet Basil-Ocimum basilicum L. (Lamiaceae), Asian J. Chem., 2011, vol. 23, p. 3773.
Rubab, S., Hussain, I., Khan, B.A., Unar, A.A., Abbas, K.A., Khichi, Z.H., Khan, M., Khanum, S., Rehman, K.U., and Khan, H., Biomedical description of Ocimum basilicum L., J. Islamic Int. Med. Coll., 2017, vol. 12, p. 59.
Shahrajabian, M.H., Sun, W., and Cheng, Q., Chemical components and pharmacological benefits of Basil (Ocimum basilicum): a review, Int. J. Food Prop., 2020, vol. 23, p. 1961. https://doi.org/10.1080/10942912.2020.1828456
Lang, E., Amelunxen, F., Friedrich, H., and Horster, H., Morphometrische Untersuchungen zur Bildung und Akkumulation von Laminaceengerbstoffen in Ocimum basilicum Zellkulturen, Planta-Med., 1978, vol. 33, p. 281.
Ambreen, M. and Mirza, S.A., Evaluation of anti-inflammatory and wound healing potential of tannins isolated from leaf callus cultures of Achyranthes aspera and Ocimum basilicum, Pak. J. Pharm. Sci., 2020, vol. 33, p. 361. https://doi.org/10.1080/15226514.2018.1524828
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The work was carried out within the framework of the thematic plan-task for the implementation of research work at the Russian State Agrarian University—Moscow Timiryazev Agricultural Academy by order of the Ministry of Agriculture of Russia at the expense of the federal budget in 2023 as well as with the support of the Ministry of Science and Higher Education of the Russian Federation in accordance with agreement no. 075-15-2022-746 dated May 13, 2022, (internal number MK-3084.2022.1.4) on the provision of a grant in the form of a subsidy from the federal budget of the Russian Federation within the framework of a grant from the president of the Russian Federation for state support of young Russian scientists—candidates of sciences, doctors of sciences—and leading scientific schools of the Russian Federation.
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Abbreviations: BAS—biologically active substances; TPC—total phenolic content; NPs—nanoparticles.
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Cherednichenko, M.Y., Polivanova, O.B., Khlebnikova, D.A. et al. Management of Biosynthetic Potential of Aseptic Plants and Callus Cultures of Ocimum basilicum L. In Vitro. Russ J Plant Physiol 70, 170 (2023). https://doi.org/10.1134/S1021443723603130
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DOI: https://doi.org/10.1134/S1021443723603130