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HPLC-MS Analysis of Ginsenosides in Morphogenic Cell Cultures of Ginseng (Panax ginseng C.A. Meyer)

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Abstract

The composition of triterpene glycosides in different in vitro tissues of cell lines (PgR–rhizogenic and PgG–hemogenic) and roots of ginseng plants (Panax ginseng C.A. Meyer), grown on plantations in Primorsky Krai (Russian Federation) was determined using the HPLC-MS method. More than 60 triterpene glycosides have been detected and characterized. The maximum content of ginsenosides was identified in the leaves of the hemogenic line. The total amount of ginsenosides in the PgG line embryonic tissue was lower compared to all other samples studied mainly due to the amount of protopanaxadiols. In all parts of the PgR line, the content of protopanaxatriol Re was two times higher than in the root of the intact plant. A high content of protopanaxatriols and oleanolic ginsenosides was found in the basal parts of PgR and PgG calli. Thus, morphogenic cell lines not only repeated the pattern of qualitative ginsenosides composition in comparison with individual parts of native roots and leaves but also demonstrated an increase in their diversity and quantity.

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ACKNOWLEDGMENTS

We used the equipment of the Center for Collective Use “Biotechnology and Genetic Engineering” of the Federal Scientific Center of the East Asia Terrestrial Biodiversity (Far Eastern Branch, Russian Academy of Sciences).

Funding

The work was carried out with financial support from the Russian Science Foundation grant no. 23-26-00213.

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Authors and Affiliations

  1. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia

    T. Yu. Gorpenchenko, V. P. Grigorchuk, Yu. V. Vologzhanina, M. T. Khandy, G. K. Chernoded & Yu. N. Zhuravlev

  2. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia

    V. V. Makhankov

  3. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia

    D. V. Kochkin

  4. Moscow State University, Moscow, Russia

    D. V. Kochkin

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  1. T. Yu. Gorpenchenko
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  2. V. P. Grigorchuk
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  3. V. V. Makhankov
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  4. D. V. Kochkin
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Correspondence to T. Yu. Gorpenchenko.

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Gorpenchenko, T.Y., Grigorchuk, V.P., Makhankov, V.V. et al. HPLC-MS Analysis of Ginsenosides in Morphogenic Cell Cultures of Ginseng (Panax ginseng C.A. Meyer). Russ J Plant Physiol 70, 172 (2023). https://doi.org/10.1134/S1021443723603087

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