Skip to main content
SpringerLink
Log in

The Determination of Secondary Metabolites of Kamchatka Honeysuckle Lonicera caerulea var. kamtschatika Sevast.

  • RESEARCH PAPERS
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

For the first time, a comprehensive analysis of polyphenolic compounds in extracts of Kamchatka honeysuckle berries (Lonicera caerulea var. kamtschatica Sevast.) was carried out. We used 5 samples of wild honeysuckle and one variety sample Elena from the collection of the Kamchatka Research Institute of Agriculture. High-resolution tandem mass spectrometry was used to identify target analytes in the extracts. 58 polyphenolic compounds corresponding to the Caprifoliaceae family have been identified. Among the identified analytes, some compounds were determined for the first time: flavones—hydroxy genistein, diosmetin O-hexoside, chrysoeriol-O-diglucoside; flavanols—herbacetin, myricetin, astragalin, taxifolin-O-hexoside, rhamnocitrin; flavan-3-ols—epiafzelechin, epicatechin-O-hexoside, epigallocatechin-epicatechin dimer; phenolic acids—hydroxyferulic acid, hydroxy methoxy dimethylbenzoic acid, neochlorogenic acid, ellagic acid; stilbenes—pinosylvin, resveratrol and many other polyphenols. The richest in the content of polyphenolic compounds was the wild form variety nos. 5–23 polyphenolic compounds, followed by the Elena variety—21 polyphenolic compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

REFERENCES

  1. Gidzyuk, I.K., Sineplodnaya sadovaya zhimolost’ (Blue-Fruited Garden Honeysuckle), Tomsk: Izd. Tomsk. Gos. Univ., 1978.

  2. Yakubov, V.V. and Chernyagina, O.A., Katalog flory Kamchatki (sosudistye rasteniya) (Catalog of the Flora of Kamchatka (Vascular Plants), Petropavlovsk-Kamchatsky: Kamchatpress, 2004.

  3. Ilyin, V.S., Blue honeysuckle, in Pomologiya. V pyati tomakh (Pomology. In Five Volumes), vol. 5: Zemlyanika. Malina. Orekhoplodnye i redkie kul’tury (Strawberries. Raspberries. Nut and Rare Crops), Orel: VNIISPK, 2014.

  4. Petrusha, E.N., Economic and biological characteristics of new varieties of honeysuckle bred by the Kamchatka Research Institute of Agriculture, Plod. Yagod. Ros., 2019, vol. 58, p. 273. https://doi.org/10.31676/2073-4948-2019-58-273-278

    Article  Google Scholar 

  5. Petrusha, E.N., Study of the main biochemical components of honeysuckle in the Kamchatka region, Vest. Ros. s-kh. n., 2021, vol. 4, p. 32. https://doi.org/10.30850/vrsn/2021/4/32-34

  6. Solovyova, T.A. and Petrusha, E.N., Zhimolost’ na Kamchatke (Honeysuckle in Kamchatka), Petropavlovsk-Kamchatsky: Kamchatpress, 2004.

  7. Kuklina, A.G., Sorokopudov, V.N., Upadyshev, M.T. Sorokopudova, O.A., and Prishchepina, G.A., State and prospects of blue honeysuckle breeding, Vest. Ros. s-kh. n., 2017, vol. 5, p. 41.

  8. Sofronov, A.P., Firsova, S.V., and Golovunin, V.P., Zhimolost’ sinyaya (Lonicera caeruleae L.): tekhnologiya i selektsiya (Blue Honeysuckle (Lonicera caeruleae L.): Technology and Selection), Kirov: FGBNU FANC North-East, 2021.

    Google Scholar 

  9. Pharmacopoeia of the Eurasian Economic Union, Approved by the decision of the board of the Eurasian Economic Commission dated August 1, 2020, No. 100.

  10. Wojakowska, A., Piasecka, A., Garcia-Lopez, P.M., Zamora-Natera, F., Krajewski, P., Marczak, L., Kachlicki, P., and Stobiecki, M., Structural analysis and profiling of phenolic secondary metabolites of Mexican lupine species using LC–MS techniques, Phytochem., 2013, vol. 92, p. 71. https://doi.org/10.1016/j.phytochem.2013.04.006

    Article  CAS  Google Scholar 

  11. Hamed, A.R., El-Hawary, S.S., Ibrahim, R.M., Abdelmohsen, U.R., and El-Halawany, A.M., Identification of chemopreventive components from halophytes belonging to Aizoaceae and Cactaceae through LC/MS—bioassay guided approach, J. Chrom. Sci., 2021, vol. 59, p. 618. https://doi.org/10.10193/chromsci/bmaa112

    Article  CAS  Google Scholar 

  12. Abeywickrama, G., Debnath, S.C., Ambigaipalan, P., and Shahidi, F., Phenolics of selected cranberry genotypes (Vaccinium macrocarpon Ait.) and their antioxidant efficacy, J. Agr. Food Chem., 2016, vol. 64, p. 9342. https://doi.org/10.1021/acs.jafc.6b04291

    Article  CAS  Google Scholar 

  13. Jaiswal, R., Muller, H., Muller, A., Karar, M.G.E., and Kuhnert, N., Identification and characterization of chlorogenic acids, chlorogenic acid glycosides and flavonoids from Lonicera henryi L. (Caprifoliaceae) leaves by LC-MSn., Phytochem., 2014, vol. 108, p. 252. https://doi.org/10.1016/j.phytochem.2014.08.023

    Article  CAS  Google Scholar 

  14. Cai, Z., Wang, C., Zou, L., Liu, X., Chen, J., Tan, M., Mei, Y., and Wei, L., Comparison of multiple bioactive constituents in the flower and the caulis of Lonicera japonica Based on UFLC-QTRAP-MS/MS combined with multivariate statistical analysis, Molecules, 2019, vol. 24, p. 1936. https://doi.org/10.3390/molecules24101936

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Aita, S.E., Capriotti, A.L., Cavaliere, C., Cerrato, A., Giannelli Moneta, B., Montone, C.M., Piovesana, S., and Lagana, A., Andean blueberry of the genus Disterigma: a high-resolution mass spectrometric approach for the comprehensive characterization of phenolic compounds, Separations, 2021, vol. 8, p. 58. https://doi.org/10.3390/separations8050058

    Article  CAS  Google Scholar 

  16. Zhao, Y., Lu, H., Wang, Q., Liu, H., Shen, H., Xu, W., Ge, J., and He, D., Rapid qualitative profiling and quantitative analysis of phenolics in Ribes meyeri leaves and their antioxidant and antidiabetic activities by HPLC-QTOF-MS/MS and UHPLC-MS/MS, J. Sep. Sci., 2021, vol. 44, p. 1404. https://doi.org/10.1002/jssc.202000962

    Article  CAS  PubMed  Google Scholar 

  17. Li, X. and Tian, T., Phytochemical characterization of Mentha spicata L. under differential dried-conditions and associated nephrotoxicity screening of main compound with organ-on-a-chip, Front. Pharmacol., 2018, vol. 9, p. 1067. https://doi.org/10.3389/fphar.2018.01067

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Pandey, R. and Kumar, B., HPLC–QTOF–MS/MS-based rapid screening of phenolics and triterpenic acids in leaf extracts of Ocimum species and their interspecies variation, J. Liq. Chromatogr. Relat. Technol., 2016, vol. 39, p. 225. https://doi.org/10.1080/10826076.2016.1148048

    Article  CAS  Google Scholar 

  19. Rafsanjany, N., Senker, J., Brandt, S., Dobrindt, U., and Hensel, A., In vivo consumption of cranberry exerts ex vivo antiadhesive activity against fimH-dominated uropathogenic Escherichia coli: a combined in vivo, ex vivo, and in vitro study of an extract from Vaccinium macrocarpon, J. Agric. Food Chem., 2015, vol. 63, p. 8804. https://doi.org/10.1021/acs.jafc.5b03030

    Article  CAS  PubMed  Google Scholar 

  20. Ieri, F., Martini, S., Innocenti, M., and Mulinacci, N., Phenolic distribution in liquid preparations of Vaccinium myrtillus L. and Vaccinium vitis idaea L., Phytochem. Anal., 2013, vol. 24, p. 467. https://doi.org/10.1002/pca.2462

    Article  CAS  PubMed  Google Scholar 

  21. Abu-Reidah, I.M., Ali-Shtayeh, M.S., Jamous, R.M., Arraes-Roman, D., and Segura-Carretero, A., HPLC–DAD–ESI-MS/MS screening of bioactive components from Rhus coriaria L. (Sumac) fruits, Food Chem., 2015, vol. 166, p. 179. https://doi.org/10.1016/j.foodchem.2014.06.011

    Article  CAS  PubMed  Google Scholar 

  22. Mena, P., Calani, L., Dall’Asta, C., Galaverna, G., Garcia-Viguera, C., Bruni, R., Crozier, A., and Del Rio, D., Molecules, 2012, vol. 17, p. 14821. https://doi.org/10.3390/molecules171214821

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Burgos-Edwards, A., Jimenez-Aspee, F., Theoduloz, C., and Schmeda-Hirschmann, G., Colonic fermentation of polyphenols from Chilean currants (Ribes spp.) and its effect on antioxidant capacity and metabolic syndrome-associated enzymes, Food Chem., 2018, vol. 30, p. 144. https://doi.org/10.1016/j.foodchem.2018.03.053

    Article  CAS  Google Scholar 

  24. Yin, N.-W., Wang, S.-X., Jia, L.-D., Zhu, M.-C., Yang, J., Zhou, B.-J., Yin, J.-M., Lu, K., Wang, R., Li, J.-N., and Qu, C.-M., Identification and characterization of major constituents in different-colored rapeseed petals by UPLC−HESI-MS/MS, Agric. Food Chem., 2019, vol. 67, p. 11053. https://doi.org/10.1021/acs.jafc.9b05046

  25. Ismail, W.M., Ezzat, S.M., Aliaa, E.M.K., El-Mosallamy, Kadriya, S., El Deeb, and El-Fishawy, A.M., In vivo antihypertensive activity and UHPLC-Orbitrap-HRMS profiling of Cuphea ignea A. DC., ACS omega, 2022, vol. 7, p. 46524. https://doi.org/10.1021/acsomega.2c05356

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Liu, P., Lindstedt, A., Markkinen, N., Sinkkonen, J., Suomela, J., and Yang, B., Characterization of metabolite profiles of leaves of bilberry (Vaccinium myrtillus L.) and lingonberry (Vaccinium vitis-idaea L.), J. Agric. Food Chem., 2014, vol. 62, p. 12015. https://doi.org/10.1021/jf503521m

    Article  CAS  PubMed  Google Scholar 

  27. Razgonova, M.P., Tekutyeva, L.A., Podvolotskaya, A.B., Stepochkina, V.D., Zakharenko, A.M., and Golokhvast, K.S., Zostera marina L. supercritical CO2-extraction and mass spectrometric characterization of chemical constituents recovered from seagrass, Separations, 2022, vol. 9, p. 182. https://doi.org/10.3390/separations9070182

    Article  CAS  Google Scholar 

  28. Flamini, R., Recent applications of mass spectrometry in the study of grape and wine polyphenols, Int. Sch. Res. Notices, 2013. https://doi.org/10.1155/2013/813563

  29. Chen, Y., Cai, X., Li, G., He, X., Yu, X., Yu, X., Xiao, Q., Xiang, Z., and Wang, C., Chemical constituents of radix Actinidia chinensis planch by UPLC–QTOF–MS, Biomed. Chromatogr., 2021, vol. 35, p. e5103. https://doi.org/10.1002/bmc.5103

    Article  CAS  PubMed  Google Scholar 

  30. Singh, J., Kumar, S., Rathi, B., Bhrara, K., and Chhikara, B.S., Therapeutic analysis of Terminalia arjuna plant extracts in combinations with different metal nanoparticles, J. Mater. NanoSci., 2015, vol. 2, p. 1.

    Google Scholar 

  31. Ruiz, A., Hermosin-Gutierrez, I., Vergara, C., von Baer, D., Zapata, M., Hitschfild, A., Obando, L., and Mardones, C., Anthocyanin profiles in south Patagonian wild berries by HPLC-DAD-ESI-MS/MS, Food Res. Int., 2013, vol. 51, p. 706. https://doi.org/10.1016/j.foodres.2013.01.043

    Article  CAS  Google Scholar 

  32. Fermo, P., Comite, V., Sredojevic, M., Ciric, I., Gasic, U., Mutic, J., Baosic, R., and Tesic, Z., Elemental analysis and phenolic profiles of selected Italian wines, Foods, 2021, vol. 10, p. 158. https://doi.org/10.3390/foods10010158

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Ruiz, A., Hermosin-Gutierrez, I., Mardones, C., Vergara, C., Herlitz, E., Vega, M., Dorau, C., Winterhalter, P., and von Baer, D., Polyphenols and antioxidant activity of calafate (Berberis microphylla) fruits and other native berries from Southern Chile, Agric. Food Chem., 2010, vol. 51, p. 706. https://doi.org/10.1021/jf100173x

    Article  CAS  Google Scholar 

  34. Zengin, G., Mahomoodally, M.F., Sinan, K.I., Ak, G., Etienne, O.K., Sharmeen, J.B., Brunetti, L., Leone, S., Di Simone, S.C., Recinella, L., and Chiavaroli, A., Chemical composition and biological properties of two Jatropha species: different parts and different extraction methods, Antioxidants, 2021, vol. 10, p. 792. https://doi.org/10.3390/antiox10050792

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Razgonova, M.P., Bazhenova, B.B., Zabalueva, Y.Y., Burkhanova, A.G., Zakharenko, A.M., Kupriyanov, A.N., Sabitov, A.S., Ercisli, S., and Golokhvast, K.S., Rosa davurica Pall., Rosa rugosa Thumb., and Rosa acicularis Lindl. originating from Far Eastern Russia: Screening of 146 chemical constituents in tree species of the genus Rosa, Applied Sci., 2022, vol. 12, p. 9401. https://doi.org/10.3390/app12199401

    Article  CAS  Google Scholar 

  36. Bujor, O.-C., Extraction, identification and antioxidant activity of the phenolic secondary metabolites isolated from the leaves, stems and fruits of two shrubs of the Ericaceae family, PhD (Biol.) Thesis, 2016.

  37. Said, R.B., Hamed, A.I., Mahalel, U.A., Al-Ayed, A.S., Kowalczyk, M., Moldoch, J., Oleszek, W., and Stochmal, A., Tentative characterization of polyphenolic compounds in the male flowers of Phoenix dactylifera by liquid chromatography coupled with mass spectrometry and DFT, Int. J Mol. Sci., 2017, vol. 18, p. 512. https://doi.org/10.3390/ijms18030512

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Ekeberg, D., Flate, P.-O., Eikenes, M., Fongen, M., and Naess-Andresen, C.F., Qualitative and quantitative determination of extractives in heartwood of Scots pine (Pinus sylvestris L.) by gas chromatography, J. Chromatogr. A, 2006, vol. 1109, p. 267. https://doi.org/10.1016/j.chroma.2006.01.027

    Article  CAS  PubMed  Google Scholar 

  39. Zhu, X.X., Yang, L., Li, Y.J., Zhang, D., Chen, Y., Kostecka, P., Kmonickova, E., and Zidek, Z., Effects of sesquiterpene, flavonoid and coumarin types of compounds from Artemisia annua L. on production of mediators of angiogenesis, Pharmacol. Rep., 2013, vol. 65, p. 410.

    Article  CAS  PubMed  Google Scholar 

  40. Guo, K., Tong, C., Fu, Q., Xu, J., Shi, S., and Xiao, Y., Identification of minor lignans, alkaloids, and phenylpropanoid glycosides in Magnolia officinalis by HPLC-DAD-QTOF-MS/MS, J. Pharm. Biomed. Anal., 2019, vol. 170, p. 153. https://doi.org/10.1016/j.jpba.2019.03.044

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

The study was carried out at the Federal State Budgetary Scientific Institution “Federal Research Center All-Russian Institute of Plant Genetic Resources named after. N. I. Vavilov”, funded by the Russian Science Foundation grant no. 23-74-00044.

Author information

Authors and Affiliations

  1. N. I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000, St. Petersburg, Russia

    M. P. Razgonova

  2. Kamchatsky Scientific Research Institute of Agriculture, 684033, Sosnovka, Russia

    E. N. Petrusha & E. A. Rusakova

  3. Siberian Federal Scientific Centre of Agrobiotechnology, Centralnaya, Presidium, 633501, Krasnoobsk, Russia

    K. S. Golokhvast

Authors
  1. M. P. Razgonova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. N. Petrusha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. A. Rusakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. S. Golokhvast
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to M. P. Razgonova, E. N. Petrusha, E. A. Rusakova or K. S. Golokhvast.

Ethics declarations

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

This work does not contain any studies involving human and animal subjects.

CONFLICT OF INTEREST

The authors of this work declare that they have no conflicts of interest.

Additional information

Publisher’s Note.

Pleiades Publishing remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Razgonova, M.P., Petrusha, E.N., Rusakova, E.A. et al. The Determination of Secondary Metabolites of Kamchatka Honeysuckle Lonicera caerulea var. kamtschatika Sevast.. Russ J Plant Physiol 70, 177 (2023). https://doi.org/10.1134/S1021443723603063

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1134/S1021443723603063

Keywords:

Search

Navigation