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The Effect of Pseudomonas putida and Spermine on Growth and Bioactive Metabolites of Hemerocallis fulva L. Leaves

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Abstract

The daylily is a perennial monocot herb with significant ornamental value. The daylily is used as food and has medicinal properties. This research was aimed to evaluate the role of Pseudomonas putida and spermine on bioactive metabolites of Hemerocallis fulva L. (daylily). Two years old daylily’s leaves were sprayed with 72 hours old culture of Pseudomonas putida and spermine (10–5 mM) at its vegetative phase. Plants were grown under natural conditions. Six months after treatments, growth, biochemical parameters and, bioactive metabolites viz. phenolics, flavonoids, terpenoids, and phytohormones were determined. Phytohormones indole acetic acid (IAA), and gibberellic acid (GA) contents were analyzed through HPLC. Both spermine and Pseudomonas putida significantly increased the plant’s growth and biochemical content, but P. putida was more effective. Spermine enhanced the number of leaves, GA content, protein, proline, phenolics, flavonoids, fresh and dry weight by 29.03, 15, 22.22, 59.84, 32.21, 38.25, 24.23, 45.58, and 39.29% respectively as compared to control. P. putida showed a similar increase. However, P. putida showed maximum increase in auxin content. The combined treatment of spermine and P. putida showed maximum increase in the growth, GA, protein, proline, phenolics, and terpenoids contents of the plants. The ROS scavenging activity by H. fulva showed different behavior at different concentrations. It is inferred from the present results that combined application of Pseudomonas putida and spermine can be implicated in horticulture and pharmaceutical industries to increase yield and the production of the bioactive metabolites by daylily.

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ACKNOWLEDGMENTS

We are thankful to Dr. Syed Waqas Hassan, Chairperson, Department of Biosciences, University of Wah for his cooperation and facilitation. The authors also acknowledge the Lab complex University of Wah Rawalpindi Pakistan for providing technical support.

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Authors and Affiliations

  1. Department of Biosciences, University of Wah, 44070, Wah Cantt, Pakistan

    Mahnoor Asghari Bano & Jehangir Khan

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  1. Mahnoor Asghari Bano
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Correspondence to Mahnoor Asghari Bano.

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Abbreviations: ABTS—2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid); PGPR—plant growth promoting rhizobacteria; PGR—plant growth regulator.

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Bano, M., Khan, J. The Effect of Pseudomonas putida and Spermine on Growth and Bioactive Metabolites of Hemerocallis fulva L. Leaves. Russ J Plant Physiol 69, 132 (2022). https://doi.org/10.1134/S1021443722060024

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  • DOI: https://doi.org/10.1134/S1021443722060024

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