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Three MTP Transporters Sequestrate Zn in Sedum alfredii Hance

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Abstract

Hyperaccumulating ecotype (HE) of Sedum alfredii Hance is a Zn/Cd hyperaccumulator, which can accumulate Zn in shoot up to 2% of dry weight, understanding the mechanism of Zn tolerance and accumulation can improve its application in phytoremediation. In this study, the function of two metal tolerance protein (MTP) genes of HE plants (SaMTP2h and SaMTP3h) was comparatively analyzed with the known SaMTP1. Three transporters could complement Zn sensitivity in yeast mutant in different levels. mRNA level of SaMTP1 in shoot was constitutively and highly expressed which was thousands of times of other two genes. mRNA level of SaMTP1 was not affected by Zn/Cd treatment, while mRNA levels of SaMTP2h and SaMTP3h were up-regulated by 50 µM Cd or 500 µM Zn treatment. SaMTP2h was significantly higher expressed in young leaves while SaMTP1 and SaMTP3h were relatively equally expressed in different leaves; SaMTP1 was higher expressed in mesophyll and SaMTP3h was significantly higher expressed in epidermis. Overexpression of SaMTP1, SaMTP2h or SaMTP3h significantly enhanced Zn tolerance and accumulation in tobacco plants and complemented Zn sensitivity in Arabidopsis mtp1-1 mutant. In conclusion, our study revealed that three MTPs could play significant but different roles in Zn accumulation and tolerance in HE plants.

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ACKNOWLEDGMENTS

We are grateful to Prof. David Eide for providing us the CM100 strain and Δzrc1 mutant strain.

Funding

The present study was supported by National Natural Science Foundation of China (project no. 3130183).

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Authors and Affiliations

  1. Zhejiang Key Lab of Exploitation and Preservation of Coastal Bio-Resource, Wenzhou Key Laboratory of Marine Biological Genetics and Breeding, Zhejiang Mariculture Research Institute, 325005, Wenzhou, Zhejiang, China

    M. Zhang & D. W. Ji

  2. International Center for Environmental Membrane Biology, Department of Horticulture, Foshan University, 528000, Foshan, China

    J. Zhang

  3. Oujiang College, Wenzhou University, 310058, Wenzhou, Zhejiang, China

    R. T. Jiao

  4. MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China

    X. E. Yang

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  1. M. Zhang
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  2. J. Zhang
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  3. R. T. Jiao
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  4. X. E. Yang
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  5. D. W. Ji
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Correspondence to D. W. Ji.

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Conflict of interests. The authors declare that they have no conflicts of interest.

Statement on the welfare of humans or animals. This article does not contain any studies involving animals performed by any of the authors.

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Abbreviations: HE—hyperaccumulating ecotype; HMA—heavy metal ATPase; MTP—metal tolerance protein; NHE—non-hyperaccumulating ecotype.

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Zhang, M., Zhang, J., Jiao, R.T. et al. Three MTP Transporters Sequestrate Zn in Sedum alfredii Hance. Russ J Plant Physiol 68, 1115–1124 (2021). https://doi.org/10.1134/S1021443721060212

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  • DOI: https://doi.org/10.1134/S1021443721060212

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