Effects of Temperature on Transcriptome Profiles in Cardiocrinum cathayanum Leaves

Abstract

Cardiocrinum cathayanum (Endl.) Lindl. (Liliaceae) is a promising species for ornamental and pharmaceutical usage. However, genomic responses of C. cathayanum to temperature have not been investigated. In the present study, C. cathayanum leaves were cultivated at 10, 20, and 30°C, and leaves were collected for transcriptome sequencing. Overall, 36.9 to 44.1 M clean reads were obtained, which finally assembled 48 120 unigenes. Among these unigenes, 49.39% could be annotated to at least one public database. Compared with 20°C treatment, 381 and 303 unigenes were significantly upregulated, 580 and 1399 were significantly downregulated in 10 and 30°C treatments, respectively. Real-time qPCR analyses on selected 12 unigenes showed similar tendencies to those calculated on transcriptome sequencing. Compared with 20°C treatment, exposure to 10°C significantly affected the pentose and glucuronate interconversions, starch and sucrose metabolism pathways. Further exploration suggested that D-galacturonate and trehalose were accumulated at 10°C, which would enhance the tolerance to cold stress. Besides, 10°C treatment inhibited the biosynthesis of unsaturated fatty acids and α-linolenic acid metabolism. These changes might negatively affect C. cathayanum growth. At 30°C, 40 of 42 differentially expressed genes (DEGs) in the starch and sucrose metabolism, all the 27 DEGs in the glycolysis/gluconeogenesis and all the 17 DEGs in the fructose and mannose metabolism were significantly downregulated, in comparison to 20°C treatment. These changes demonstrated that high temperature greatly inhibited carbohydrate metabolism in C. cathayanum leaves. Overall, the present study contributed new insights to understand the genomic responses of C. cathayanum leaves to various temperatures.

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ACKNOWLEDGMENTS

We thank the Shenzhen GenProMetab Biotechnology Company for their commentary and the language corrections in the present manuscript.

Funding

This work was supported by the Natural Science Foundation of Educational Committee of Anhui Province (project no. KJ2016A682), the Key Project of Visiting and Studying Inland and Aboard Program for Young Scholars (project no. gxfxZD2016234), the Scientific Reseach Foundation of Huangshan University (project no. 2018xkjq013), and the Open Research Project of Anhui Simulation Design and Modern Manufacture Engineering Technology Research Center in Huangshan University (project no. SGCZXYB03).

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Contributions

X. F. Wang and Z. B. Wan designed the experiments. Y. J. Ye, M. Y. Fan, L. Chen and T. Ma collected samples and performed the experiments. X. F. Wang and Z. B. Wan drafted the manuscript and all authors revised it.

Corresponding author

Correspondence to X. F. Wang.

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This article does not contain any studies involving animals or human participants as objects of research. The authors declare that they have no conflict of interest.

Additional information

Abbreviation: COG/KOG—cluster of orthologous groups of proteins database / clusters of orthologous groups for eukaryotic complete genomes; DEGs—differentially expressed genes; FDR—fault detection rate; GO Gene ontology; JA—jasmonates; KEGG—Kyoto encyclopedia of genes and genomes; log2FC—log2 fold change; Nr—non-redundant protein; RH— relative humidity; RIN—RNA integrity number; RPKM— reads per kilobase of exon per million reads mapped; 12-OPDA—12-oxo-phytodienoic acid.

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Wang, X.F., Ye, Y.J., Fan, M.Y. et al. Effects of Temperature on Transcriptome Profiles in Cardiocrinum cathayanum Leaves. Russ J Plant Physiol 67, 1105–1115 (2020). https://doi.org/10.1134/S1021443720060199

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Keywords:

  • Cardiocrinum cathayanum
  • differentially expressed gene
  • growth
  • Gene Ontology
  • KEGG pathway
  • hot stress
  • cold stress